KERN Optics OBN 158 User Instructions Download Page 29 | Manualshive

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OBN-13-BA-e-1510

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Overview of wave lengths for excitation and emission per excitation filter

Blue line: Wave length excitation
Green line: Wave length emission

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Summary of Contents for OBN 158

Page 1: ...H Ziegelei 1 D 72336 Balingen E mail info kern sohn com Tel 49 0 7433 9933 0 Fax 49 0 7433 9933 149 Internet www kern sohn com User instructions Transmitted light laboratory microscope KERN OBN 13 OBN 132 OBN 135 Version 1 0 01 2015 ...

Page 2: ......

Page 3: ... Condenser 12 5 Operation 12 5 1 Getting started 12 5 2 Pre focussing 13 5 3 Adjusting the interpupillary distance 14 5 4 Dioptre adjustment 14 5 5 Adjusting the magnification 15 5 6 Adjusting the Koehler illumination 16 5 7 Using eye cups 19 5 8 Using oil immersion objectives 20 6 Changing the bulb 21 7 Changing the fuse 21 8 Using optional accessories 22 8 1 Polarisation unit 22 8 2 Camera conne...

Page 4: ...an additional risk of triggering an electric shock 1 2 Notes on the electrical system Before connecting to a mains power supply you must make sure that you are using the correct input voltage The information to select the correct mains cable is located on the device on the rear of the product directly above the connection socket You must comply with this information If you do not comply with these...

Page 5: ...hich are too high or too low vibrations dust or a high level of humidity The ideal temperature range is between 0 and 40 C and a relative humidity of 85 should not be exceeded The device should always be located on a rigid smooth horizontal surface When the microscope is not being used you should cover it with the enclosed dust protective cover When doing this the power supply is stopped by switch...

Page 6: ...as these are highly flammable substances You must therefore keep it away from naked flames and electrical devices which can be switched on and off and only use it in well ventilated rooms However organic solutions of this type should not be used to clean other components of the device This could lead to damage to the paint finish To do this it is sufficient to use a neutral cleaning product You co...

Page 7: ...perture adjustment Condenser Condenser Field lens with Field diaphragm Control dial X Y axis Specimen stage Fine adjustment knob Coarse adjustment knob Dimmer Tube connectors with dioptre adjustment ring Centring screw Condenser Centring screw Condenser Adjusting screw Condenser Camera adapter connection with adjusting screw Trinocular toggle rod ...

Page 8: ...7 OBN 13 BA e 1510 Rear view Adjusting ring torque Carrying handle Main switch Power connection Coarse adjustme nt knob Fine adjustment knob Fuse Condenser Focus dial ...

Page 9: ...nity Trinokular 6V 20W Halogen Transmitting OBN 135 Infinity Trinokular 3W LED Transmitting Eyepieces WF 10x Ø 20 mm Objectives 4x 10x 20x 40x 100x Product dimensions 390x200x400 mm Packaging dimensions 570x310x410 mm Net weight 9 kg Gross weight 11 kg Input voltage AC 100 240V 50 60Hz Output voltage DC 1 2 6V Fuse 2A 5x20 mm ...

Page 10: ...9 OBN 13 BA e 1510 ...

Page 11: ...OBN 13 BA e 1510 10 4 Assembly ...

Page 12: ...rongest magnification appears You must make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures For objectives which are marked OIL you must use an immersion oil with the lowest level of inherent fluorescence 4 3 Eyepieces You must always use eyepieces with the same magnification for both eyes These are simply placed onto the tube connectors once you ...

Page 13: ...accessories 5 Operation 5 1 Getting started The very first step is to establish a power connection using the mains plug You should first adjust the dimmer to a low level so that when you look through the eyepiece for the first time your eyes are not immediately subject to a high level of light You can now switch on the lighting using the main switch The next step is to place an object holder with ...

Page 14: ... soon as an image is recognisable no matter how sharp then you should only adjust the focus using the fine adjustment knob Adjusting the torque of the coarse and fine adjustment knob Next to the left adjustment wheel for the coarse and fine adjustment knob there is a ring which you can use to alter the torque of these wheels Turning it in a clockwise direction reduces the torque and turning it in ...

Page 15: ...illary distance is set correctly 5 4 Dioptre adjustment The eye strengths of each eye of the microscope user can often be slightly different which in daily life has no consequences But when using a microscope this can cause problems in achieving precise focussing You can use a mechanism on both tube connectors dioptre adjustment rings to compensate for this as follows 1 Put the right dioptre adjus...

Page 16: ... objectives you should use the silver ring above the objectives see illustration When rotating the nosepiece you must always make sure that the objective which is about to be positioned in the beam path does not touch the object holder This can lead to significant damage to the objective lens We recommend that you always check from the side to make sure that there is sufficient leeway If this shou...

Page 17: ...phragm When adjusting the Koehler lighting for the first time you must first select the lowest possible objective magnification so that you can carry out the following steps 1 Use the condenser focus wheel to position the condenser directly below the specimen stage Switch on the lighting and use the coarse and fine adjustment knob to bring the slide with the cover glass positioned facing upwards i...

Page 18: ...the condenser up so high that it collides with the object holder Therefore care is needed when doing this 4 Use the centring screws of the condenser holder to bring the image from the field diaphragm into the centre of the field of view 5 Open the field diaphragm until it just disappears out of the field of view If necessary simply re centre using the centring screws on the condenser holder ...

Page 19: ...2 3 of the pupil diameter If the eyepiece should be removed for checking then please make sure that no dirt or dust falls into the tube 7 It is possible to alter the brightness of the bulb using the dimmer The brightness is always controlled by the bulb brightness and not by the aperture diaphragm 8 Possibly there is the need of re adjusting the focus and x y axis 9 Observe the object If another m...

Page 20: ...sses symbol on the side They are also marked in the item description by an additional H example HSWF 10x Ø 23 mm When fitting the eye cups make sure that the dioptre setting is not moved We would therefore advise that you hold the dioptre compensation ring on an eyepiece with one hand while you fit the eye cup with the other Before using the microscope users who wear glasses must remove the eye cu...

Page 21: ...2 Lower the specimen stage and position the 100x objective in the beam path 3 Bring the specimen stage or object slide to the objective very slowly until there is slight contact 4 Observe the object The object slide and objective must not be pressed against each other The oil constitutes the contact layer If the contact is made too jerky there is a chance that existing air bubbles in the oil canno...

Page 22: ...ulb can be pulled out of the socket and be replaced with a new one After the bulb holder has been replaced in the underside of the device and the screws replaced the bulb replacement procedure is complete Important When fitting the new bulb into the socket it must only be handled with sterile gloves or using the bulb packaging film Grease and dust residue can have a negative effect on the light qu...

Page 23: ...iece see illustration on page 10 where you can simply insert the analyser see left illustration The polariser on the other hand is only placed on the ring holder of the field lens see right illustration There are two points to take into account when subsequently using the polarisation unit The aperture setting of the condenser must be in position PH For its starting position the polariser must be ...

Page 24: ...ing the microscope camera i e using the PC screen which is connected To do this the trinocular toggle rod on the righthand side of the microscope head must be pulled out The light from the microscope lighting is deflected so that it is completely in the beam path for the camera which causes a dark field of view in the eyepieces This means that it is not possible to simultaneously observe by the ey...

Page 25: ...t This consists of a simple PH condenser a PH objective with a specific magnification 10x 20x 40x or 100x a PH slider which is adapted to the lens being used a centring telescope and two green filters To use this you need to replace the standard condenser of the microscope with the PH condenser One of the objectives in the nosepiece is also replaced with the PH objective and this is positioned in ...

Page 26: ...ces of the user using a green filter can produce a more effective and pleasant image To achieve this the green filter must be screwed onto the underside of the PH condenser 2 Comprehensive phase contrast unit This consists of a PH universal rotary condenser four PH objectives 10x 20x 40x and 100x a centring telescope two centring turnscrews and a green filter To use this you need to replace the st...

Page 27: ...d a black objective ring The black one is central and the white one possible is pushed to one side see left illustration g Now press the turnscrews fitted on the side of the condenser towards the centre until they grip the screws Then by turning the screws and at the same time watching the rings through the centring telescope move the white ring to the centre see central illustration h As soon as ...

Page 28: ...ntained in the spectrum of the light source HBO or LED The excitation filter is only permeable for the according wave length After that the excitation light hits a dichroic mirror which reflects it towards the objective and the sample After the sample absorbed the excitation light the emission of the fluorescent light occurs with a bigger wave length than the excitation light The part of the fluor...

Page 29: ...OBN 13 BA e 1510 28 Overview of wave lengths for excitation and emission per excitation filter Blue line Wave length excitation Green line Wave length emission ...

Page 30: ...29 OBN 13 BA e 1510 ...

Page 31: ...OBN 13 BA e 1510 30 ...

Page 32: ...ver for FL module Fixing Allen screw for microscope head Main body Connection point for microscope head Fixing screw for lamp housing 7 8 9 10 11 HBO LED lamp housing Power connection Screw for attaching the protective plate Connection point for microscope housing Allen wrench ...

Page 33: ... device for field diaphragm Control lever for field diaphragm Centring device for aperture diaphragm Control lever for aperture diaphragm 6 7 8 9 10 Condenser control Screw for cover of lamp housing Centring screw for lamp vertical Centring screw for lamp horizontal Control lever for illumination ...

Page 34: ...flected light units available for the microscopes of the series OBN 13 They have the following specifications Article number Illumination Excitation filter Centring objective Input voltage power supply unit OBB A1155 100W HBO UV V B G Yes 100 240V OBB A1153 100W HBO B G Yes 100 240V OBB A1156 3W LED B G Yes 100 240V ...

Page 35: ...the reflected light unit for special fluorescence applications it will be favourable to adjust the microscope in the bright field mode This includes the placing of the sample adjusting the interpupillary distance pre focussing dioptre adjustment etc After that you can go on with the usage of the fluorescence reflected light unit 1 When using a HBO lamp at first there must be ensured that the contr...

Page 36: ...t reduction of the lamps life time Equally the lamp must not be switched on directly after a switch off process When pausing the observation you should always slide in the control lever for the illumination in order to interrupt the light beam The light spectrum of the HBO lamp often can be harmful for microbes Never look inside the eyepieces when the beam path is opened by the control lever for i...

Page 37: ...5 Use the two centring screws on the lamp housing for moving the light spot into the centre of the hair cross Warning Heat development on the lamp housing Changing the lamp HBO 1 Disconnect the FL reflected light unit from the power supply 2 Check if the lamp housing has cooled down 3 Loosen the screw for the cover of the lamp housing only when it is cool 4 Carefully flip out the lamp holder 5 Loo...

Page 38: ...the mains power supply socket With the device switched off and unplugged you can screw out the housing The defective fuse can be removed from its housing and be replaced with a new one After that you just need to insert the fuse housing back into the insertion point next to the mains power supply socket ...

Page 39: ...tly centred The condenser is too low The field of view is dark or is not correctly illuminated The objective is not positioned correctly on the beam path The selector switch for the beam path is between two settings The nosepiece is not correctly fitted The condenser is not correctly fitted An objective is being used which doesn t match the lighting area of the condenser The condenser has not been...

Page 40: ... Dirt dust on the front lens of the condenser Dirt dust on the object One side of the image is blurred The stage was not correctly fitted The objective is not positioned correctly on the beam path The nosepiece is not correctly fitted The upper side of the object is facing down The image flickers The nosepiece is not correctly fitted The objective is not positioned correctly on the beam path The c...

Page 41: ...ly materials which you can dispose of at your local recycling centre Disposal of the storage box and device must be carried out by the operator in accordance with all national or regional laws in force in the location of use 12 Further information The illustrations may differ slightly from the product The descriptions and illustrations in this user manual are subject to change without notice Furth...

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