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8.  Technical  Information 

 

RA-620/RA-600 

41 

 

About refractive index of water (nD) 

 
The following list shows relative refractive index (nD) of water at various temperatures 
(Celsius) with light source of sodium Na-D line. 
 

Temp. 

(

C) 

RI 

(nD) 

Temp. 

(

C) 

RI 

(nD) 

Temp. 

(

C) 

RI 

(nD) 

Temp. 

(

C) 

RI 

(nD) 

1.33388 

25 

1.33250 

45 

1.32985 

65 

1.32628 

1.33385 

26 

1.33240 

46 

1.32969 

66 

1.32609 

1.33382 

27 

1.33229 

47 

1.32953 

67 

1.32589 

1.33378 

28 

1.33217 

48 

1.32937 

68 

1.32568 

1.33373 

29 

1.33206 

49 

1.32920 

69 

1.32548 

10 

1.33369 

30 

1.33194 

50 

1.32904 

70 

1.32527 

11 

1.33363 

31 

1.33182 

51 

1.32887 

71 

1.32507 

12 

1.33358 

32 

1.33170 

52 

1.32870 

72 

1.32486 

13 

1.33352 

33 

1.33157 

53 

1.32852 

73 

1.32464 

14 

1.33345 

34 

1.33144 

54 

1.32835 

74 

1.32443 

15 

1.33338 

35 

1.33131 

55 

1.32817 

75 

1.32422 

16 

1.33331 

36 

1.33117 

56 

1.32799 

 

 

17 

1.33324 

37 

1.33104 

57 

1.32781 

 

 

18 

1.33316 

38 

1.33090 

58 

1.32762 

 

 

19 

1.33307 

39 

1.33075 

59 

1.32744 

 

 

20 

1.33299 

40 

1.33061 

60 

1.32725 

 

 

21 

1.33290 

41 

1.33046 

61 

1.32706 

 

 

22 

1.33280 

42 

1.33031 

62 

1.32687 

 

 

23 

1.33271 

43 

1.33016 

63 

1.32668 

 

 

24 

1.33261 

44 

1.33001 

64 

1.32648 

 

 

Allan H. Harvey, John S. Gallagher, J. M.H. Levelt Senger "Revised Formulation for the 
Refractive Index of Water and Steam as a Function of Wavelength, Temperature, and 
Density" J. Phys. Chem. Ref. Data Vol.27, No.4 pp.761-774 (1998) 
 
 

About Brix % 

 
Brix  %  means  mass  concentration  %  of  sucrose;  however,  it  is  often  used  as 
concentration  %  of  soluble  solid  matter  contained  in  aqueous  solution.  Soluble  solid 
means all substances including sugar, salt, protein, etc. that are soluble in water. 

 

Though  you  can  obtain  the  actual  concentration  with  sucrose  solution,  you  may  not 
necessarily obtain the concentration of sucrose solution when the solution contains other 
types of soluble solid matter.   

Summary of Contents for RA-620

Page 1: ...Refractometer RA 620 RA 600 Operation Manual P 5 9 0 0 0 3 3 0 1 Read this operation manual thoroughly before use AN 59 00033 01Ver 10...

Page 2: ......

Page 3: ...y Operation 4 7 inch colour LCD touchscreen operation Information message to guide you to next step Display in English Korean Chinese or Russian Easy Maintenance Easy to clean sample stage Clean and d...

Page 4: ...ditions to avoid malfunction Caution Under vibration Under direct sun light Corrosive gas atmosphere Heavily loaded and fluctuated or near power source or magnetic field Excessive range of temperature...

Page 5: ...e you leave the unit unused for an extended period of time It is recommended to use the carton box in which the unit was first delivered Avoid very hot or humid ambient or a dusty place for a long ter...

Page 6: ...covered by warranty 2 Hint This symbol notes technical tips which are convenient to your measurement work It is prohibited to duplicate any part or all of manual without prior consent This manual has...

Page 7: ...00 Series Operation Manual CD ROM 1 59 00035 01 RA 600 Series Quick Manual 1 59 00405 Safety Instructions 1 20 05627 Inspection Certificate Warranty 1 50 00761 Contact 1 59 00133 Unpack the carton 1 1...

Page 8: ...Set Up Conditions Temperature Contents 18 4 Measurement 19 4 1 Sample Measurement 19 4 2 Read Data Store in USB Flash Drive 21 4 3 Check 23 4 4 Calibration 25 5 Function Tools 28 5 1 Function 28 6 Mai...

Page 9: ...t Measure P 19 Stop measurement P 20 Measurement Data Read the data P 21 Download data to USB flash drive P 21 Set up condition Check Calibrate Set up measuring condition P 18 Configure Contents nD Br...

Page 10: ...2 Touchscreen Sample Cover Anti Volatilization Device Sample Stage Prism As measurement temperature is set to high the sample stage and prism will warm up accordingly Note The stopper on sample cover...

Page 11: ...and Parts USB Connector Exhaust Vent LAN Connector Power Switch Power Connector 1 3 Supplied Parts and Installation Intake Vent 2 2 Change Filter RS 232C Connector COM2 RS 232C Connector COM1 Bottom...

Page 12: ...See separate Function Description Result Shows measure results When pressed it shows a list of results See 4 2 Read Data Store in USB Flash Drive Menu When pressed it turns to window for selection of...

Page 13: ...pressed window for setup of option appears See separate Function Description Indicator Shows the indicator When pressed it turns to window for setup of indicator See separate Function Description Info...

Page 14: ...No See Function Description Rinse Purge Press here to start rinse purge See Function Description Purge Press here to start purge Switches to Skip when changer is operating See Function Description Pr...

Page 15: ...een Enter with Alphabet Numeric To confirm entry that you have entered press Enter key The Cap Small key switches capital small letter Up to 16 characters can be entered except for sample ID up to 32...

Page 16: ...window Returns to previous window Tab buttons Switch the tabs turns the page For details of operation see the separate Function Description Note To operate the touch screen lightly touch with stylus o...

Page 17: ...thermal difference between the inside and outside of unit Thermal equilibrium stabilizes electronics of precision parts and is ideal status for correct measurement Suggested warming up time is about...

Page 18: ...water Distilled water You need to be careful when using the following types of water Ion exchanged water Tap water Cautions 1 Make sure that the results become the same as what are obtained with refra...

Page 19: ...ng off sample on prism 1 Tissue paper or paper waste Tissue fiber should not break and its debris must not remain on prism After sample is wiped off tissue fiber debris should not remain on prism To c...

Page 20: ...r Conduct a calibration accordingly For details refer to 4 4 Calibration Contents to select 1 Press Contents button on main window 2 Press Contents button to select Selection from registered concentra...

Page 21: ...rect measurement cannot be expected 2 Drop sample more than 0 2mL over the prism Stir sample evenly before dropping onto prism Note A small amount of sample may result in inaccurate measurement with s...

Page 22: ...on display Immediately wipe off the sample after measurement and clean the prism and the sample stage Leaving the sample there for a long time may make it difficult to clean the prism and the sample s...

Page 23: ...u wish to save mark appears When pressed again it disappears changes the page Press if you wish to select all You can review details of data when the data is selected Up to 300 data can be stored More...

Page 24: ...Automatically folder RA6xx is created and stored in CSV format When data reading is underway all buttons stop functioning Note Remove USB flash drive device only after saving data is complete Never r...

Page 25: ...w Calibration Navigator will go to calibration See 4 4 Calibration 3 Drop about 0 2mL check water on prism Note Before dropping sample clean sample stage and prism Otherwise correct measurement cannot...

Page 26: ...the set temp The measurement time may get longer without a warm up operation See 3 1 Warming up for details Check reference value or criterion can be set on Menu Check Calibration Check Calibration Se...

Page 27: ...ding navigation Be sure to clean the prism No sample residue should remain on prism 1 Press Check When the display says Calibration required the Calibration button will appear instead of Check Pressin...

Page 28: ...ior to calibration with air Otherwise correct measurement cannot be expected Note Be sure to close sample cover in order to shut out incoming rays for correct measurement 4 Drop water onto prism Close...

Page 29: ...Setup Calibration data are stored in calibration record You can review on Menu Data management Calibration record For details refer to the separate Function Description Measurement can be performed w...

Page 30: ...ow is a convenient tool to practice exciting features of the unit To start with let us learn about Function window itself 1 Press Menu 2 Press Function 3 Select Select the item to be worked on from Cr...

Page 31: ...lue can be corrected by this feature This is a convenient tool when measurement results with different value from RI of other refractometer or standard liquid of refractive index Save import parameter...

Page 32: ...on on measurements collectively Setup provides configuration on the unit for convenience which includes Date and time Colour Sound Display mode International Temperature control and Version Security l...

Page 33: ...measurement Before measurement 1 Check the unit with water or calibrate as necessary Refer to 4 3 check 4 4 Calibration After measurement 1 Clean sample stage and prism thoroughly Refer to 3 3 Prepare...

Page 34: ...rd liquid and Brix converted RI standard liquid are available from KEM See 6 4 Consumables and parts 3 If repeated measurements result with an error deviated from range in 8 3 Specifications consult w...

Page 35: ...d filter 3 Wash the filter with water or vacuum it 4 When washed then dry the filter well 5 If dust remains even after rinsed change the filter with new one 6 Reinstall the filter and cover Dustproof...

Page 36: ...918 RA 620 RA 600 Operation Manual CD ROM Operation Manual Function Description Operation Manual for RS 232C Quick Manual 12 03678 Filter Set 5sheets Dustproof filter 5 filters set Refractive index st...

Page 37: ...meas range Exceeding meas range Sample measurement exceeds specified range Measure a sample within specified range Results may appear even though range error but just for reference only Error message...

Page 38: ...Insert USB flash drive and try again USB writing error Unable to write Writing in USB flash drive has failed due to USB error Change USB flash drive No enough space in USB drive No more space in USB...

Page 39: ...ature or room temperature When the set temperature is above 75 C reduce ambient temperature to 25 C or below Measurement record list and details Print data window Error message at time of Measurement...

Page 40: ...Stir well the sample in advance before dropping onto sample stage Homogenize the sample 3 Set stability sense to 1 or 2 Quickly measure before colloidal particles go down Spread in multiple layers e...

Page 41: ...ioning properly 1 Lower the set temperature as much as possible 2 Adjust the sample temperature close to the set temperature Shorten temperature control time and measure quickly Gap between set temp a...

Page 42: ...of light is and refraction angle these two angles are related as follows Snell s law Eq n n 1 2 sin sin If light travels from thick to thin medium the light also changes its direction As the angle of...

Page 43: ...31 36 1 33117 56 1 32799 17 1 33324 37 1 33104 57 1 32781 18 1 33316 38 1 33090 58 1 32762 19 1 33307 39 1 33075 59 1 32744 20 1 33299 40 1 33061 60 1 32725 21 1 33290 41 1 33046 61 1 32706 22 1 33280...

Page 44: ...57 1 43520 82 1 49597 8 1 34477 33 1 38661 58 1 43743 83 1 49862 9 1 34629 34 1 38846 59 1 43967 84 1 50129 10 1 34782 35 1 39032 60 1 44193 85 1 50398 11 1 34937 36 1 39220 61 1 44420 12 1 35093 37...

Page 45: ...9 1 41041 1 41040 4 1 33877 1 33876 1 33875 48 1 41447 1 41438 1 41437 6 1 34171 1 34170 1 34169 50 1 41851 1 41841 1 41840 8 1 34470 1 34468 1 34467 52 1 42260 1 42250 1 42249 10 1 34772 1 34771 1 34...

Page 46: ...IDP 100 Print out of measurement results Thermal Printer DP 600 Epson A4 Printer DCU 551N Auto Clean Unit 1 sample Automated measurement and CHD 502N Multiple Sample Changer 30 samples cleaning Flow C...

Page 47: ...s Septum CHD 502N CHD 502N Multiple Sample Changer w Vials Septum 12 03018 Flow Cell Unit 12 02997 Measurement Cell Cover For volatility sample 12 03265 Data Acquisition Software Connection Cable requ...

Page 48: ...programmed conversion table Concentration By Conversion Table 100 data Interfaces LAN x 1 Personal computer PC USB1 1 x 2 USB flash drive keyboard barcode reader Epson inkjet printer 8 Thermal Printer...

Page 49: ...sed by excessive force applied to glassware or plastics malfunction or leakage is caused by sample properties corrosivity solid materials etc malfunction is caused by any device part and or chemical o...

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