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(3)
Remounting the sample chamber
a) Locate the sample chamber over the two positioning pins on the main unit and
move it all the way to the back.
b) Ensure that light is incident upon the center of the cell holder. To check it
visually, set the wavelength to 500 nm and place a piece of white paper in front of
the cell holder.
c) Firmly tighten the two fixing screws and close the lid.
4.3
Performance Check: Data Station Type
A performance check should be
run
approx. once a month to ensure the
normal
instrument performance. If the instrument is not operating properly, conduct checks
and take corrective action after referring to the troubleshooting chart in Section 5. After
taking corrective action, run the performance check again to verify that the instrument
has been restored to normal.
Note: This section provides a simple description of operating procedures. For
details, refer to the Operations manual.
Baseline flatness
Refer to Section 4.3.1
Wavelength repeatability
Refer to Section 4.3.2
Wavelength accuracy
Refer to Section 4.3.3
4.3.1 Baseline (Abs 0 line) Flatness
This check should be performed more than one hour after turning the instrument on.
(1)
Start the [Spectrum Measurement] program from the [Spectra Manager] window.
(2)
Click [Measurement]-[Spectrum Parameters...] and set parameters as follows:
V-530 measurement parameters
V-550/560/570
measurement
parameters
Photometric Mode Abs
Photometric Mode Abs
Response
Medium
Response
Medium
Scanning Speed
200 nm/min
Band Width
2.0 nm
Start
1000 nm
Scanning Speed
200 nm/min
End
200 nm
Start
850 nm
Data Pitch
1.0 nm
End
200 nm
Display
-0.005~+0.005
Data Pitch
1.0 nm
No of Cycles
1
Display
-0.005~+0.005
No of Cycles
1
(3)
Measure the baseline as follows.
a) Click [Measurement]-[Baseline...]. The [Baseline Correction] dialog box
appears.
b) Click the <Measure...> button to display the [Baseline Measurement] dialog
box.
c) Make sure that the sample chamber is empty and then click the <Start> button.
The baseline is measured and stored in the memory of the spectrophotometer.
