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Transmission Microscopy 

Light is transmitted through the sample. (For transparent or translucent samples)

 

 

 

Step 1. Turning on the illumination unit. 

 
Turn on the illumination unit by pressing the on-off switch. 

 

 

Step 2. Setting the slide. 

 
Place the desired sample slide on the stage, and push it under the 
holding clamps. Alternatively, press down on the back of the holding 
clamp 

(see red arrow).

 

 

Step 3. Centering the slide. 

 
Adjust the sample slide, so that the sample is in the light cone from the 
illumination unit. 

 

Step 4. Focusing the image. 

 
Look through the eyepiece and focus image by slowly lowering the stage 
using the focusing knob. 
 
Focusing knob 

 

 

Step 5. Increasing the magnification. 
 

Change objective to 10x or 40x, or change the eyepiece to 16x WF 
Ocular for a more detailed view. Readjust the focus as necessary.  

 
Step 6. Perfecting the image. 
 

After having selected the desired magnification, try to increase or 
decrease the illumination intensity and to rotate the aperture adjustment 
wheel. Readjust the focus as necessary.  
 

A smaller aperture opening increases the contrast, but reduces resolution. A larger aperture 
opening increases the resolution, but reduces contrast. 

 

Aperture 

diaphragm 

Aperture 

Adjustment 

wheel 

Summary of Contents for ST-80

Page 1: ...ST 80 MICROSCOPE With Electronic Eyepiece 6810 User Guide...

Page 2: ...and capture images or videos onto your computer The ST 80 Microscope makes studying plant and insect samples easy and fun Caution This device includes some sharp objects Adult supervision is required...

Page 3: ...stage with the focusing knob until it cannot go any further Focusing knob Step 4 Setting the illumination unit Spin the illumination wheel to the lowest setting Step 5 Connecting the power Connect the...

Page 4: ...t Step 4 Focusing the image Look through the eyepiece and focus image by slowly lowering the stage using the focusing knob Focusing knob Step 5 Increasing the magnification Change objective to 10x or...

Page 5: ...the cap back on the light pen Step 2 Attaching the light pen Insert light pen into the opening for light pen and turn on the light pen Adjust the light pen until the beam is over the opening on the st...

Page 6: ...ualDub 1 9 7 32Bit folder or VirtualDub64 exe in VirtualDub 1 9 7 64Bit folder If you do not wish to keep running the software from the CD feel free to copy the VirtualDub 1 9 7 32Bit folder or Virtua...

Page 7: ...ed section of your experiment and make an individual video Under File Menu in Export select Image Sequence to show the selected section of your experiment in a series of image files Set the desired Fi...

Page 8: ...dling or unauthorized repair Further product malfunction or deterioration due to normal wear is not covered by this warranty IOPTRON DISCLAIMS ANY WARRANTIES EXPRESS OR IMPLIED WHETHER OF MERCHANTABIL...

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