Run Conditions for Gels
Introduction
The estimated run duration for Novex
®
1.0 mm thick mini-gel is listed below. A
1.5 mm thick gel will take 5-10 minutes longer than similar 1.0 mm thick gel.
Gel Type
Voltage
Approximate Duration
4% Tris-Glycine
125 V
90 minutes
6% Tris-Glycine
125 V
95 minutes
8% Tris-Glycine
125 V
100 minutes
10% Tris-Glycine
125 V
105 minutes
12% Tris-Glycine
125 V
110 minutes
14% Tris-Glycine
125 V
110 minutes
16% Tris-Glycine
125 V
115 minutes
18% Tris-Glycine
125 V
120 minutes
4-12% Tris-Glycine
125 V
105 minutes
4-20% Tris-Glycine
125 V
110 minutes
8-16% Tris-Glycine
125 V
105 minutes
10-20% Tris-Glycine
125 V
120 minutes
10% Tricine
125 V
70 minutes
16% Tricine
125 V
90 minutes
10-20% Tricine
125 V
75 minutes
10% Zymogram
125 V
100 minutes
12% Zymogram
125 V
110 minutes
4-16% Zymogram
125 V
100 minutes
6% TBE
100 V
65 minutes
10% TBE
100 V
100 minutes
20% TBE
200 V
105 minutes
4-20% TBE
200 V
70 minutes
6% TBE-Urea
180 V
45 minutes
10% TBE-Urea
180 V
60 minutes
15% TBE-Urea
180 V
80 minutes
IEF pH 3-7, Step 1
100 V
60 minutes
IEF pH 3-7, Step 2
200 V
60 minutes
IEF pH 3-7, Step 3
500 V
30 minutes
IEF pH 3-10, Step 1
100 V
60 minutes
IEF pH 3-10, Step 2
200 V
60 minutes
IEF pH 3-10, Step 3
500 V
30 minutes
NuPAGE
®
Novex 10% Bis-Tris with MES SDS Running Buffer
200 V
35 minutes
NuPAGE
®
Novex 4-12% Bis-Tris with MES SDS Running
Buffer
200 V
35 minutes
NuPAGE
®
Novex 10% Bis-Tris with MOPS SDS Running
Buffer
200 V
50 minutes
NuPAGE
®
Novex 4-12% Bis-Tris with MOPS SDS Running
Buffer
200 V
50 minutes
NuPAGE
®
Novex 7% Tris-Acetate
150 V
60 minutes
NuPAGE
®
Novex 3-8% Tris-Acetate
150 V
70 minutes
18
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