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Invitrogen PowerEase 500, Instructions For Use Manual

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Invitrogen PowerEase 500 Instructions For Use Manual Download Page 20

Using PowerEase

®

 500 

  

Introduction 

You can operate the PowerEase

®

 500 Power Supply immediately after 

installation using three simple steps (see below). For programming custom 
settings, see page 13. 

 

Starting 
PowerEase

®

 500  

1.  Plug the power cord of the PowerEase

®

 500 Power Supply into an available 

electrical outlet rated at the appropriate electrical values for the power 
supply. 

2.  Turn on the main power switch located on the rear of the PowerEase

®

 500 

Power Supply (see page ix for rear view of PowerEase

®

 500 Power Supply). 

Always leave this switch in the 

ON

 position to keep the back-up battery fully 

charged. 

3. Press 

the 

Power

 button located on the keypad (see page x for a figure of the 

keypad). This 

Power 

button is a secondary power button and does not 

actually shut the instrument down, but shuts down the high voltage, fan, 
and LCD screen. When powered back up, the LCD screen will revert to the 
same screen as when the secondary power was shut off. 

4. A 

Start-Up

 screen will appear (see next page).  

To avoid condensation, the main power switch located on the rear of the unit 
should be left on between use during operation in a cold room or in high 
humidity environments. 

 

Operating 
PowerEase

®

 500  

A simple operating procedure of the PowerEase

®

 500 Power Supply is provided 

below. For more details on the different screens and custom methods, see pages 
5-13. 

1.  Turn on the PowerEase

®

 500 Power Supply. 

2. Press 

Select

 once the 

Start-Up

 screen appears. 

3.  Select your gel type from the 

Gel Method

 screen. 

4.  Choose the number of gels to be electrophoresed from the 

Gel Quantity

 

screen. 

5. Press 

the 

Start

 button to begin electrophoresis. 

Note

: To reset the PowerEase

®

 500 Power Supply, press the 

Stop

 button. 

  

 

The maximum number of Novex

®

 mini-gels that can be electrophoresed at the 

same time using PowerEase

®

 500 Power Supply and the XCell 

SureLock

™

 Mini-

Cell is listed below: 

Gel Type 

Number of Gels 

Novex

®

 Tris-Glycine Gels 

10 gels 

NuPAGE

®

 Novex Tris-Acetate Gel 

6 gels 

NuPAGE

®

 Novex Bis-Tris Gels 

2 gels 

Novex

®

 Tricine Gels 

4 gels 

Novex

®

 TBE-Urea gels 

10 gels 

Continued on next page 

  

5 

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Summary of Contents for PowerEase 500

Page 1: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

Page 2: ...34 PowerEase 500 Power Supply A programmable power supply for electrophoresis Catalog nos EI8600 EI8700 EI8675 www invitrogen com tech_service invitrogen com Artisan Technology Group Quality Instrumen...

Page 3: ...ii Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Page 4: ...diate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential...

Page 5: ...iv Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Page 6: ...iv Introduction 1 Overview 1 Methods 3 Getting Started 3 Operational Modes 4 Using PowerEase 500 5 Printing 12 Custom Methods 13 Troubleshooting 16 Appendix 17 Warranty 17 Run Conditions for Gels 18 R...

Page 7: ...ction Manual 1 each Extra Fuses 2 each Power Cord U S Canada Europe Taiwan and Japan 1 each Warranty Card 1 each Temperature Probe Optional 1 each See next page for specifications and detailed descrip...

Page 8: ...in 8 hours after 30 minute warm up with constant supply voltage Circuit Protection Open Circuit Short Circuit Thermal Protection Over Voltage Current Wattage Safety Load Detection Thermal Shutoff with...

Page 9: ...1 LCD Screen 2 Power Output Jacks Positive Red 3 Power Output Jacks Negative Black 4 Temperature Probe Jack 5 Functional Keys Power Start Stop 6 Directional Keys Up Down Left Right 7 High Voltage LED...

Page 10: ...werEase 500 PRINTER RS232 Fan RS232C Communi cation Port Printer Port Power Entry Module with On Off main power switch Continued on next page ix Artisan Technology Group Quality Instrumentation Guaran...

Page 11: ...ct a menu choice and to move forward to the next applicable screen Left directional button for moving left around the menu Right directional button for moving right around the menu Up directional butt...

Page 12: ...unded AC outlet Avoiding Damage to the Instrument 1 For proper ventilation leave at least 10 cm of space behind the instrument and at least 5 cm of space on each side 2 Do not operate the power supply...

Page 13: ...res avant d effectuer toute connection 4 Utiliser des prises de courant munies d une connection la terre Pour viter d endommager l appareil 1 Assurer une ventilation correcte en laissant un minimum d...

Page 14: ...le sauber und rocken ist Sie sollten niemals mit nassen H nden Ger t Kabel oder Steckverbindungen anfassen 4 Schlieben Sie das PowerEase 500 nur an eine einwandfrei geerdete 220 230V Strombuchse an Um...

Page 15: ...Service see page 21 or visit our Web site at www invitrogen com Product Quantity Catalog no Temperature Monitoring Probe 1 EI8644 RS232 Cable and Adaptor 1 EI8663 Fuses 1A 250V 3AGL Fast Blow 100 120V...

Page 16: ...werEase 500 Power Supply are listed below Large LCD display allows electrical parameters to be displayed in either graphical or numerical formats Eight pre programmed methods for running and blotting...

Page 17: ...ON Start Up Screen Gel Method Screen Gel Quantity Screen Method Run Large Display Stop Select Method Edit Screen Method Run Large Display Previous Screen Stop Start Previous Screen Select once Select...

Page 18: ...Printer located on the rear of the PowerEase 500 Power Supply 2 Connect the opposite end of the cable into the appropriate connector on the printer Refer the user manual of your printer for instructio...

Page 19: ...ply the voltage will increase as resistance increases to satisfy Ohm s law V IR see page 20 If no voltage limit is set and a local fault condition occurs such as a poor connection very high local resi...

Page 20: ...see next page To avoid condensation the main power switch located on the rear of the unit should be left on between use during operation in a cold room or in high humidity environments Operating Powe...

Page 21: ...page if a pre programmed method is chosen or to the Custom Method Option screen see page 13 if a user defined method is selected Note The pre programmed method durations are set to the shortest run t...

Page 22: ...Gel Method screen or the Custom Method Options screen if a custom method is in use If the number of gels selected causes the current to be scaled such that the PowerEase 500 Power Supply maximum powe...

Page 23: ...sent Step Number Actual Wattage small display Detected Temperature if installed Pressing the Select button once takes you to the Method Run Graphic Display screen see next page and to the Method Run E...

Page 24: ...used Present Run Time Voltage Current and Wattage applied during the run Run Condition Limiting Parameter The Select button takes you to the Method Run Edit screen see page 11 The Stop button pauses t...

Page 25: ...s must be in the range of 1 to 500 volts 1 to 500 mA and 0 1 to 50 watts for one gel the values for current and watts will be reduced if multiple gels are selected If you have changed a pre programmed...

Page 26: ...mber of Gels Total Time Elapsed Run Condition limiting parameter or error condition if detected Detected Temperature if probe is installed Present Step Number Present Parameter Set Points Step Time El...

Page 27: ...s STOP quits is displayed at the bottom of the screen 3 Press the Start button to print a report reflecting voltage current watts and temperature changes which occurred during the run An example of a...

Page 28: ...hod name Save Method Edit or Run Method Edit or Run Method The Edit or Run Method option displays the Gel Quantity screen see figure below for the pre programmed methods Enter the number of gels to be...

Page 29: ...e the current character only capital letters numbers 0 to 9 and a few other characters such as and are available 2 Press the Start button to delete the character directly under the cursor and all rema...

Page 30: ...en press the Select button 2 The custom method is cleared and the Gel Method screen is displayed Choosing No and pressing the Select button or pressing the Stop button returns you to the Custom Method...

Page 31: ...el buffers are prepared correctly and the recommended volume of buffer is added to the electrophoresis cell Restart the method Display shows PAUSE and Hardware Error Reset unit by holding front panel...

Page 32: ...during the warranty period This warranty does not apply if the product has been damaged by accident abuse misuse or misapplication or from ordinary wear and tear For your protection items being return...

Page 33: ...100 minutes 12 Zymogram 125 V 110 minutes 4 16 Zymogram 125 V 100 minutes 6 TBE 100 V 65 minutes 10 TBE 100 V 100 minutes 20 TBE 200 V 105 minutes 4 20 TBE 200 V 70 minutes 6 TBE Urea 180 V 45 minute...

Page 34: ...ower switch at the rear of the PowerEase 500 Power Supply and detach the power cord from the rear of the PowerEase 500 Power Supply 2 Open the fuse compartment located inside the Power Entry Module se...

Page 35: ...t the resistance of the gel increases as the chloride glycine front moves down the gel and the current decreases Voltage The velocity in which an ion moves in an electric field will vary in proportion...

Page 36: ...ur site for easy reference Contact Us For more information or technical assistance please call write fax or email Additional international offices are listed on our Web page www invitrogen com Corpora...

Page 37: ...service in house repair center WE BUY USED EQUIPMENT Sell your excess underutilized and idle used equipment We also offer credit for buy backs and trade ins www artisantg com WeBuyEquipment REMOTE IN...

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