40
Troubleshooting,
Continued
Problem
Cause
Solution
Plasticware incorrectly
loaded on the iPrep
™
Rack
Do not just place the cartridge on the rack. You need to
slide the cartridge into the rack and push down as
described on page 25.
While loading the tubes on the iPrep
™
Tip and Tube Rack,
be sure the tubes are
uncapped
.
If the plasticware are incorrectly loaded, the purification
protocol still starts as there are no sensors on the
instrument to detect incorrect placements. Once the
protocol begins, the piercing unit may collide with the
incorrectly placed cartridges or the tips may encounter a
barrier (when capped tubes are inserted) which triggers an
error message causing the instrument to stop.
Protocol stops
after an initial
start
iPrep
™
Racks
incorrectly loaded on
the instrument
Be sure to load the iPrep
™
Cartridge Rack first followed by
the iPrep
™
Tip and Tube Rack as described.
If the iPrep
™
Racks are incorrectly loaded, the purification
protocol still starts as there are no sensors on the instrument
to detect incorrect placements. Once the protocol begins, the
instrument may experience liquid handling problems which
triggers an error message causing the instrument to stop.
Bubbles formed
during
purification
Missed adding sample
or sample volume is
lower than the
recommended volume
Be sure to add the sample to tubes prior to starting the
protocol. To ensure proper mixing of reagents in the tip
and prevent bubble formation during mixing, make sure
the sample volume is at least the recommended volume
listed in the manual supplied with iPrep
™
Kits.
Filter barrier on
the tip is wet
Missed adding sample
or sample volume is
lower than the
recommended volume
Be sure to add the sample to sample tubes and the sample
volume must be at least the recommended volume listed in
the manual supplied with the iPrep
™
Kit to prevent bubble
formation. Bubbles can wet the filter barrier on the tip,
resulting in inefficient dispensing with the tips that may
affect the yield.
Presence of
buffer in the
Bottom Tray
Motor movements may
not be smooth,
incorrect placement of
plasticware, or leakage
from tips
Perform preventive maintenance annually to ensure proper
motor movements.
If you are loading less than 13 cartridges, ensure that the
remaining plastic ware (tips and tubes) are also loaded in
the same order as the cartridges to prevent any spillage.
See below for leakage from tips.
Leakage from
tips or uneven
liquid handling
between nozzles
D-Rings are not
greased regularly or are
very old
Perform routine maintenance of the D-Rings (applying
grease and changing the D-Rings) are described on page 38
for proper performance of the instrument and prevent any
leakages.
Blockage of tips
Too much starting
material causing
clumps or aggregates
Decrease the amount of starting material. Use the
recommended amount of starting material as listed in the
iPrep
™
Kit manual.
Continued on next page
