31
Using the iPrep™ Purification Instrument,
Continued
Performing the
Purification
Protocol
After assembling the iPrep
™
Racks with components, start the purification
protocol.
1.
Close the instrument door.
The instrument does not work if the door is open.
2.
Select the appropriate elution volume on the display.
3.
Confirm that you have selected the correct purification protocol, elution
volume and have loaded the plasticware and racks properly. Ensure that the
sample tube
without lids
(containing the sample) and elution tubes
without
caps
are placed in the correct positions on the iPrep
™
Tip and Tube Rack (see
page 26 for details).
4.
Press
Start
. The automated purification protocol begins and various steps of
the protocol including the approximate time remaining are displayed on the
digital display.
Important: Do not open the door once the protocol has begun.
To pause or stop the
protocol, see next page.
5.
At the end of the run, the instrument beeps briefly and digital display shows
Protocol Finished
for 10 seconds. The Main menu appears after 10 seconds.
6.
Open the instrument door. Remove and cap the elution tubes containing the
purified nucleic acid. Store the purified nucleic acid appropriately as described
in the iPrep
™
Kit manual.
7.
Discard the used cartridges, tips, and sample tube into biohazard waste. Do
not reuse the cartridges.
8.
To process more samples using the same iPrep
™
Protocol Card, load the racks
with new cartridges, tips, tubes, and sample, and start the protocol as
described above.
Continued on next page
