IEC Centra-CL3 Series Operation Manual Download Page 1

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Summary of Contents for Centra-CL3 Series

Page 1: ...60 60 Hz k I Cenbifuge 9 t No 3765 2 V C 60 Hz C tJ ff C 37 6 a29 j f 240 VAC 50 Hz t o 37 7 aa tVA 60 Hz df 11 r UALITY IEC CENTRIFUGES V Jl IEC INTERNATIONAL EQUIPMENT COMPANY 300 SECOND AVENUE NEEDHAM HEIGHTS MA 02194 U SA TEL 781 449 8060 TOLL FREE 800 843 1113 FAX 781 444 6743 ...

Page 2: ...otors including fixed angle swinging bucket and fixed horizontal designs They can process tubes bottles microplates microcapillary tubes cytological slide carriers and microsample tubes The units can centrifuge up to one liter offluid in a single operation Each centrifuge has an easy to use front panel that provides two modes of operation Manual and Programmed Manual mode is used for entering temp...

Page 3: ...rting and Stopping a Run 17 3 3 Stored Programs 20 3 4 Centra CL3 Series Refrigeration 21 3 5 Diagnostic Messages and Error Codes 23 Chapter 4 Applications 4 1 Introduction 25 4 2 Speed and Force Tables 27 4 3 Derating for Dense Samples Table 36 4 4 Chemical Resistance Table 39 4 5 Decontamination Table 40 4 6 RCF Nomograph 41 Chapter 5 Maintenance 5 1 Introduction 43 5 2 Care and Cleaning 43 5 3 ...

Page 4: ...nt ofa power failure The rugged steel cabinet and rigid construction provide quiet operation and long term reliability 1 2 About This Manual The Operations Manual contains all ofthe information needed to install operate and maintain a CL3 Series centrifuge Refrigerated and ventilated models operate similarly and any differences are highlighted and noted throughout this manual This manual also cont...

Page 5: ...ses against certain actions or situations that could result in personal injury A Caution advises against actions or situations that could damage equipment produce inaccurate data or invalidate a procedure A Note provides useful infonnation regarding an operation function or procedure Centra CL3 Series Operation Manual 3 ...

Page 6: ...a CL3 ventilated model can be placed in a cold room no colder than 0 C for processing temperature sensitive samples When you remove the centrifuge from a cold environment do not operate for a minimum oftwo hours so that any condensation will evaporate Note When used in a cold room environment some bearing noise may become evident The bearing lubricant thickens at low temperatures As the centrifuge...

Page 7: ...an dry surface to make certain that the suction feet at the bottom grip the surface firmly Keep the area beneath the unit free of debris and loose materials The resting surface must be level to ensure quiet vibration free operation A rigid and stable location is important An improperly loaded centrifuge may vibrate or move al Jling 1nternationa1 lectrot hnlcal G nunission t3 4 rcf i no part g 20 A...

Page 8: ...tions may sag when the refrigeration system turns on The unit requires a grounded power supply 3 prong outlet Ifyour facility does not have grounded power outlets arrange for a proper grounding The power cord plugs in on the left rear side ofthe unit o i ili t e powerplug tbatdoes not have gr Undilig pin The powercord provided with the tiJlltiscorrectly rated forthe highest current demand This pow...

Page 9: ...ore moving the unit 2 Position a flat object such as a tongue depressor near a suction cup at the bottom ofthe unit 3 Lift up an edge ofthe cup and insert the flat object far enough to break the vacuum seal 4 When all four cups are disengaged lift the unit from the work surface Caution When the unit is in its new location ensure that the suction cups adhere correctly to the work surface The Front ...

Page 10: ... displays have two states or modes Actual bright display and Set dim display In Actual mode bright display they indicate current run conditions such as rotor speed or force elapsed time of or time remaining in the run actual temperature Refrigerated only In Set mode dim display the display indicates the desired settings for the run Set mode is operative whenever you use the up and down arrows brie...

Page 11: ... display warning or error messages see Section 3 5 Time display The number in the display above this symbol indicates time Below ten minutes time is displayed as minutes seconds Above ten minutes just minutes are displayed Time is set in 1 second increments from 1 59 seconds 15 second increments from 1 5 minutes 1 minute increments from 5 99 minutes In normal timed mode the system counts down from...

Page 12: ...ay Note that the radius cannot be changed to a radius larger than the maximum radius or less than the minimum radius for the selected rotor Use the arrow keys to view or change the Set parameters for Speed Force Time Temperature Refrigerated only Rotor Radius or Program The first time the key is pressed the numeric display switches from Actual readings to Set parameters without changing them Ifyou...

Page 13: ...r will coast down from 600 RPM Ifboth lights are out all braking is disabled and the rotor will coast from operating speed to a stop This key starts a run A run is governed by the Set parameters manual or programmed The associated green light blinks until the rotor reaches 95 ofthe set run speed The light stays on until the end ofthe run This key stops a run A run will also stop automatically when...

Page 14: ... trunnion rings shields buckets and carriers to within I gram and stamps the weight on each piece Check these markings whenever you interchange parts to ensure that opposite parts are matched Ensure that the total weight of samples and removable parts loaded in opposing positions are equal in weight to within 1 gram The position numbers present on many rotors and adapters identify opposing tube po...

Page 15: ...trifugal force toward the bottom ofthe tubes Failure to achieve full swing out causes vibration and premature wear of the rotor and the motor Samples oflike similar specific gravities may be processed in the same run provided that the samples ofthe same type are balanced around the rotor as though they were the only pairs in the rotor Caution Do not exceed maximum rated speed for each rotor access...

Page 16: ...te this centrifuge at observed critical speeds To install the 243 rotor 1 Press the disk located on the underside ofthe rotor to the rotor 2 Slide the rotor over the shaft until it snaps into place Release the disk 3 Pull the rotor straight up to insure a positive lock 3 PULL STRAIGHT UP ON ROTOR TO CONf IRM SECURE ATTACHMENT 0 0 2 SLIDE ONTO SHAFI AND RELEASE To remove the 243 rotor 1 Press the d...

Page 17: ...n the shaft adapter and place the rotor onto the adapter 4 Tighten the rotor locking nut with the provided wrench until it tightly engages the rotor To remove existing rotors from the shaft adapter 1 Remove any sample tubes shields buckets and other accessories from the rotor 2 Unscrew and remove the locking screw 3 Grasp the rotor and rock it front to back and side to side 4 Remove the rotor from...

Page 18: ...t that the unit be in the correct mode for the desired operation These digits and symbols are displayed above the program icon They can be any ofthe following blank The unit is in manual operation 1 99 The unit is under control ofthe displayed stored program number C The unit is set to Rapid Condition a special program discussed in Section 3 4 The display indicates the last parameters selected for...

Page 19: ...ng Mode Braking begins when the elapsed time reaches this desired setting Run time does not include braking time The spin will stop automatically at the end ofthe desired interval A run can also be stopped at any time by pressing the STOP key The settings can be changed during a manual run These changes affect the run in progress Ifthe time setting is changed during a run the unit adjusts the coun...

Page 20: ... The time display counts upward and displays the elapsed time since the START key was pressed Hold Mode HLd For hold mode operation without preset time limit set the mode so that HLd appears in the Time display Select temperature CL3R only speed g force acceleration mode and braking mode as with manual operation Press and release the START key The run starts when you press the key and stops only w...

Page 21: ...parameters Each set or program is stored and can be recalled by selecting a program number from 1 99 Programs are retained in memory even if the power is turned off When necessary a program can be modified for a particular run or changed permanently You cannot change the unit s program rotor radius or timing modes during a spin Programs can be locked by scrolling to the desired program and pressin...

Page 22: ...ong as the PROGRAM SAVE file folder key is not pressed 3 4 Centra CL3R Refrigeration Refrigerated units refrigerate the rotor chamber whenever the cover is closed and the unit is switched on Refrigeration is applied as necessary to cool the rotor chamber to the currently displayed temperature setting If you use the keyboard and momentarily display a cold temperature stepping through stored program...

Page 23: ... the chamber temperature CL3R only is above the set temperature Rapid Condition will run a rotor at 500 rpm to increase air circulation in the chamber to quickly cool the chamber to the set point When the chamber temperature is below the set temperature Rapid Condition will run the rotor at 2800 rpm to warm the chamber to the set temperature When the temperature has been reached three beeps will s...

Page 24: ... erase this message PFAIL appears if power was interrupted during a run Press START to resume the previous run Press STOP to erase this message PFAII indicates a power failure This message appears when the unit is turned back on following the failure The front panel will alternate between the PFAIL message and the remaining run time or elapsed time ifin Hold mode Error Codes require factory author...

Page 25: ...ng motor latch solenoid etc are disabled COPF Cop Watchdog OpCode Trap Error The microprocessor has sensed a Jack ofactivity from the program that controls the centrifuge The rotor will coast to a stop COP COP Watchdog Not Enabled The microprocessor COP is not enabled The rotor will coast to a stop UndFI Undefined Interrupt The microprocessor was interrupted by an undefined source The rotor will c...

Page 26: ... speeds can be found in Section 4 2 Speed And Force Tables Relative Centrifugal Force RCF or G force at a given speed varies with the rotor and with the distance away rotating radius from the shaft ofthe centrifuge center of rotation The rotating radius is measures to the furthest inside tip of the tube away from the centrifuge shaft The Speed and Force Tables indicate the maximum speed and RCF th...

Page 27: ...ack from most laboratory chemicals Prolonged exposure should be avoided by immediately removing the chemical from rotor or assembly Rotors and accessories placed in the chamber are made ofa variety of materials including aluminum and polypropylene The Chemical Resistance Table shows the suitability ofeach material with different classes of reagents Section 5 2 describes how to clean and remove cor...

Page 28: ...apter Tube Trunnion Max Max Max No of Volume Shield or Speed RCF Radius O D x Length mm Places ml Carrier rpm xg cm Common Tube Size 4 50 325 320 4100 2650 14 0 30 x 116 4 50 350 323 3800 2050 15 2 Falcon Corning Conical 4 10 310 356 4300 2750 13 2 17 2 x 113 8 50 326 320 3600 2050 14 2 30 x 116 12 10 355 356 3600 1900 13 2 17 2 x 112 12 10 366 1013 4100 2400 12 8 16 5 x 107 16 x 75 16 x 100 16 5 ...

Page 29: ... Volume Carrier Speed Places ml rpm 4 included 4000 2 49852 4000 Replacement carrier is IEC No 49852 See derating table for 244 rotor Rt P k o or ac age 58012 Max RCF xg 1800 1800 Rotor 801 6 Place Fixed Angle 45 Use reauires 50968 tapered shaft adapter Tube Max Max No of Volume Shield Speed RCF Places ml rpm xg 6 50 305 4600 2950 6 50 320 5200 3300 With IEC 315 Conical Cushion Max Radius cm 10 0 ...

Page 30: ...68 taoered shaft adapter No of Tube Max Max Max Places Volume Shield Speed RCF Radius ml rpm xg cm 12 10 356 5300 3200 10 2 12 15 303 4800 2880 11 2 12 15 302 4200 2480 12 6 R t P k o or ac age 58153 Rotor 815 24 Place Fixed Angle 33 Use requires 50968 taoered shaft adapter Tube Max Max Max No of Volume Shield Speed RCF Radius Places ml rpm xg cm 24 IO 356 4800 2860 2340 11 l 9 l 24 15 303 4200 23...

Page 31: ...2 Place Fixed Angle 35 Use reauires 50968 tapered shaft adapteri Tube Max Max Max No of Volume Adapter Speed RCF Radius 0 0 x Length mm Places ml Number rpm xg cm 12 5 8500 6060 7 5 12 x 80 IEC 2804 2840 12 1 5 2 5762 8500 4680 5 5 11x66 12 B D 5 5761 8500 4770 3960 5 915 0 8 x66 12 4 25 5760 8500 4680 3630 5 915 0 6 x66 Rotor 819 10 Place Fixed Angle 35 Use reauires 50968 tapered shaft adapter Tu...

Page 32: ...1x109 6 5 5966 8500 5650 7 0 12 1x112 6 3 5967 8500 5490 6 8 10 9 x 95 Rotor 841 12 Place Fixed Angle 45 Use reQuires 50968 tapered shaft adapter No of Tube Places Volume ml 12 1 5 2 12 B D 12 5 12 4 12 25 Adapter Max Max Max Number Speed RCF Radius rpm xg cm 8500 4680 5 8 5763 8500 4770 5 9 5763 8500 3960 4 9 5764 8500 4680 5 8 5764 8500 3630 4 5 Centra CL3 Series Operation Manual 31 O D x Length...

Page 33: ...otainers 6950 40 x 0 4 ml microtubes 6790 8532 40 x 0 25 ml microtubes 8500 6060 40 x 0 8 ml 6x50 mm glass 6870 Microtainers is a registered trademark ofBecton Dickinson Order 2 pks of adapters separately IEC 5763 and 5764 are packaged 12 pk t Outer row holds 24 tubes j Inner row holds 24 tubes Radius cm 8 35 8 45 7 45 8 35 7 25 8 35 8 45 7 45 8 35 7 25 8 00t 7 50t 8 60t 8 40 7 50 8 50 1 IEC 891 R...

Page 34: ... x 175 ml Falcon 2076 61 0 4 x 200 ml Nunc 376813 61 0 4 x 225 ml Falcon 2075 61 0 4 x 250 ml IEC 2502 2504 61 0 Also order Falcon 2090 cushions Also order IEC 5792 cushions Ultrac L mm Adapter 7228 75 7228 131 7226 131 7238 llO 7225 7230 131 7223 131 7231 7231 131 131 131 131 Centra CL3 Series Operation Manual 33 Max Max Radius RPM RCF cm xg 2750 1375 16 2 2750 1450 17 2 2750 1450 17 2 2750 1450 ...

Page 35: ...alcon Conical 7234 12 x 30 ml 25 5 106 7223 4 x 50 ml 28 6 110 7222 4 x 50 ml Falcon Conical 7231 4 x 75 ml IEC 2059 38 1 112 7221 4 x 175 ml Falcon 2074 61 0 125 4 x 250 ml IEC 2261 2051 61 0 125 Also order Falcon 2090 cushions Centra CL3 Series Operation Manual 34 Max Max Radius RPM RCF cm xg 3425 1800 13 7 3425 1925 14 7 3425 1925 14 7 3425 1925 14 7 3425 1925 14 7 3425 1976 15 1 3425 1925 14 7...

Page 36: ...mato Kit Rotor 930 Use reouires 1116 hublock adapter on 50968 tapered shaft adapter The 930 Hemato Kit can convert the CL3 Series into a micro hematocrit centrifuge providing a simple economical way to perform occasional hematocrits Blood cells are packed in about ten minutes at 7800 RPM 6200 xg Kit includes numbered 24 place rotor Max RCF xg 1125 1300 1125 1600 1375 1400 1025 1075 1575 1475 llOO ...

Page 37: ... fixed angle rotors For denser samples the maximum guaranteed speed is reduced derated by a factor from the table below Derating Factor for Specific Gravity Swingin2 Bucket 1 2 1 000 1 3 960 1 4 925 1 5 894 1 6 866 1 7 839 1 8 816 1 9 794 2 0 774 2 1 755 2 2 738 2 3 721 2 4 707 2 5 692 2 6 678 2 7 666 2 8 654 2 9 642 3 0 632 Centra CL3 Series Operation Manual 36 Fixed An le 1 000 1 000 1 000 1 000...

Page 38: ...ng factors for specific gravities greater than 3 0 S0 is the maximum specific gravity allowed before derating 1 2 or 1 5 depending on the type of rotor Sa is the actual specific gravity ofthe sample Caution Do not exceed the rated speed or specific gravity Higher speeds or specific gravities will impose unnecessary wear on the centrifuge and can cause rotor failure Wear and damage caused in this m...

Page 39: ...s is loaded with 280 grams total load of 560 grams is 4000 rpm Caution Loads greater than 280 grams weight ofthe microplate and sample must have maximum speed derated according to the following table to avoid rotor failure Derating Table for 244 Rotor Load per Carrier l rams 280 300 320 340 360 380 400 450 500 600 Greater than 600 Centra CL3 Series Operation Manual 38 Max Speed rpm 4000 3600 3300 ...

Page 40: ...stic Metal Other PC PE pp PU NL DN CN NN PS Tl SS AL MB MG RR BN VN E E E G E F N F E G G F F N F E E N E E F N N N N F N N N N N N F G G E E F E E E N E E E E E F E E G F G G G G G G F N E E E E E E N E N E E N G N G F E E E E E E G G N N G G N E G G E N E E E E E N N N F G G E N E E E N E E E E E N E E N G F N N E E E N E E E E E N N E N F F N N G E G N E E E E N N N F N G G N N E E E N E G G G ...

Page 41: ... M 100 Hydrogen Peroxide s s s s s u u u u s s s s s s u u s s u 5 Phenol Solution M u u s u u M M u M M M M M M M u s s u For Ox1d1zmg Acids see Oxidizing Agents strong PA POLYALLOMER Tl TITANIUM Autoclaving PC POLYCARBONATE SS STAINLESS STELL 12 c 20 mnin PE POLYETHYLENE AL ALUMINUM 2 ATM 15 PSIG PP POLYPROPYLENE MB MANGANESE BRONZE PU POLYURETHANE MG MAGNESIUM Household Bleach NL MODIFIED PHENY...

Page 42: ...e intersects E ta Iha ref axis is the force Q 200 12 0 a For example if lhe rotating radius is 1Ocm Q and the speed is 3 000 rpm the relative centrifugal force is 1 000 xg gravity 1i5 100 10 9 8 7 6 5 Qi II the force and Iha radius are known Iha a corresponding speed can be determined 50 To Calculate RCF 20 RCF 00001118 x r x N RCF relative centrifugal force gravhies 10 r rotating racius centimete...

Page 43: ... to extend its life Clean the sample chamber rotor and lid at the end of each work day and immediately after any spill To clean the chamber use a damp sponge warm water and a mild liquid detergent suitable for washing dishes by hand such as Ivory liquid Do not use caustic detergents or detergents that contain chlorine ions These attack metals Remove stubborn stains with a plastic scrub pad Do not ...

Page 44: ...side ofthe centrifuge IEC manufactures and finishes rotors and structural accessories to give maximum resistance to corrosion To maximize the life ofthe unit continually inspect the rotor cavities for corrosion especially if you use chloride ion solutions such as sodium chloride saline and sodium hypochlorite household bleach because these solutions attack most metals Clean the rotor rotor chamber...

Page 45: ...ts should be clean and dry Store them open to the air not in a plastic bag so that any residual moisture evaporates Face the parts upward to avoid moisture retention in the cavities Iftube breakage occurs releasing toxic infectious pathogenic or radioactive material into the unit decontaminate the chamber Rotors have sealed containers that provide aerosol containment and if used as directed keep s...

Page 46: ...ning operation as described above Repeated autoclaving seriously degrades the performance of polycarbonate sealing covers 5 3 Cover Interlock Bypass The cover will remain locked if power fails Ifyou need to remove samples from the unit before power is restored use the cover interlock bypass after the rotor has come to a stop To bypass the cover interlock 1 Unplug the centrifuge 2 Locate the hidden...

Page 47: ...hin this period from the date ofits delivery due to defects in material and workmanship and we will ship you the repaired product or its replacement at our expense You must use IEC approved rotors and accessories and genuine IEC spare parts This warranty does not apply to any instrument that has been repaired without authorization or abused THE FOREGOING OBLIGATIONS ARE IN LIEU OF ALL OTHER OBLIGA...

Page 48: ...ed 243 rotor 12 x 25 ml Universal sealed 243 rotor 20 x 15 ml Falcon Corning sealed 243 rotor 8 x 50 ml Falcon Corning sealed 243 rotor 4 x 250 ml 243 rotor 4 x Microplates 2 x Deep plates 244 rotor 1 liter 243 rotor 9 C to 40 C by 1 C0 0 8 500 RPM by 100 RPM 0 01 through 0 59 by 1 second 1 00 through 4 45 by 15 seconds 5 through 99 min by 1 min Momentary operation mode Hold mode indefinite durati...

Page 49: ...0 240V 50 60 Hz 3755 120V 60 Hz 3756 220 240V 50 Hz 3757 220 240V 60 Hz Full specification compliance is guaranteed for up to 5 line voltage specified Function guaranteed up to 10 ofline voltage 3750 5 5 AMPS 3751 3 AMPS 3755 10 AMPS 3756 5 AMPS 3757 5 AMPS 1500 BTU Hr 2700 BTU Hr 32 cm 11 in cm i in cm 1 o en C in c1r i 68 kg l JS 46 kg lOOibs 32 cm 12 5 in 37 cm 14 5 in 84 cm 33 in 49 cm 19 25 i...

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