Idexx VetTest, Operator'S Manual

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IDEXX VetTest* Chemistry Analyzer Operator’s Manual
Dilution Protocols
The ability to perform dilutions on the VetTest analyzer allows you to qualify extremely
elevated results.
Plasma and Serum Dilutions
The VetTest analyzer supports plasma and serum dilutions in two circumstances:
When a test value is outside the linearity range of the VetTest analyzer t
When the sample contains interfering substances (e.g., medications) that cause a t
nonlinear or invalid result
Plasma and Serum Dilution Tips
Perform a dilution only when a test value is accompanied by a greater than symbol
t
PSEBTIFT POUIFQBUJFOUSFQPSU
6TFOPSNBMTBMJOF BTUIFEJMVFOU t
For best results, start with a 1:1 dilution (1 part sample to 1 part saline). t
Do not exceed 10 parts saline. t
Use an accurate measuring device, such as a calibrated pipette or syringe.
t
Plasma and Serum Dilution Chart
Volumes are for example only. Parts Parts Saline = Total Parts
Parts Sample Parts Saline Total Parts (Dilution Factor)
Ú- 0 1 (undiluted sample)
Ú- Ú-
2
Ú- Ú- 3
Ú- Ú-
Ú- Ú-
Ú- Ú-
Ú- Ú-
Ú- Ú-
8
Ú- Ú-
Ú- Ú-
10
Ú- Ú- 11
Parts Saline: the number entered into the VetTest when running the diluted sample
Dilution Factor: the total number of parts in the diluted sample; the VetTest automatically
multiplies the result by this number to correct for the dilution
Preparing Plasma and Serum Dilutions
To prepare a 1:1 dilution:
Accurately measure the desired amount of plasma or serum to be diluted and 1.
gently transfer it to a sample cup.
Accurately measure an equal amount of saline and transfer it to the sample 2.
collected in step 1.
Thoroughly mix the sample and saline. 3.
Proceed to the analysis (see Running a Dilution on the VetTest Analyzer
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