Idexx Filta-Max xpress, Operator'S Manual

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14 Filta-Max xpress Operator’s Guide
To fill the buffer reservoir:
The Buffer Reservoir Kit includes one 10 L bottle and one 4 L bottle. Because of the performance of
the initial system check and buffer purge, two reservoirs are required. IDEXX suggests that the 10 L
reservoir be used for the buffer solution and the 4 L reservoir be used for reagent-grade water.
The buffer reservoirs provided by IDEXX are manufactured from heavy-duty high density
polyethylene (HDPE). Only heavy-duty reservoirs should be used in this application.
1. After mixing the buffer solution, decant it into a buffer reservoir bottle.
2. The closure cap is attached to the Pressure Elution Station by an air and liquid feed line.
Attach the buffer reservoir to the cap.
3. Ensure there is a tight seal between the reservoir body and the closure cap.
Note: When the closure cap is new, the integral seal is quite stiff and may be difficult to
tighten. After several uses, tightening the closure cap will become easier. Vacuum grease can
be used to help make tightening easier.
Performing the initial system check
Before performing sample elution, you must perform an initial system and integrity check to ensure
that no error messages are generated during sample elution. This initial step also serves to purge
reagent-grade water from the lines and replace it with buffer solution.
1. Place a blank Filter Module into a Filta-Max xpress Filter Housing. (See "Assembling the
Filter Module and filtering the sample" on page 13 for instructions.)
2. This blank Filter Module can be reused for 1 month when performing the initial system check
or when purging buffer solution from the system (see "Purging the buffer solution" on page
19 for more information).
3. Complete steps 1–8 of "Eluting the sample" on page 16 .
4. The Pressure Elution Station will run through a complete elution cycle. If no errors are
generated, continue with steps 3–6 below. If a pressure or flow error occurs, refer to
Appendix C.
5. At the end of the elution cycle, discard the liquid from the centrifuge tube.
6. You can reuse the centrifuge tube when performing the initial system check or when purging
buffer solution from the system.
7. Disconnect the Filter Housing from the Pressure Elution Station, and then disconnect the
outlet diverter fitting from the Filter Housing.
8. Clean the Swagelok QC stem on the elution station with alcohol-soaked wipes.
9. Because the system uses positive pressure to elute filters, there is limited to no risk of sample-
to-sample cross-contamination.
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10. Proceed to “Transferring Filter Modules to an alternative housing” and/or “Eluting the sample.”