13
Filta-Max
xpress
Operator’s Guide
Assembling the Filter Module and filtering the sample
1. Place the Filter Module bolt-head down into a
Filter Housing.
2. Tighten the lid until the two serial-numbered tag
holes are aligned and a gap of approximately
0.5 mm is left between the lid and the base.
3. Tighten the lid an additional 1/8 of a turn past
this point and then back again to align the tag
holes. This step correctly seats the O-ring.
4. Connect the Filter Housing to the sampling
setup by connecting the QF stem to the
sampling inlet and the QC body to the sampling
outlet.
Note:
The QC body on the Filter Housing is self-
sealing and does not require an additional seal.
5. Turn on the water supply and restrict the flow
to 1–2 liters per minute using a suitable flow
restrictor located downstream of the Filter Housing.
6. When sampling is complete, turn off the water supply.
7. Disconnect the Filter Housing from the sampling setup, and seal the QF stem using the
stopper provided.
Note:
You can perform sample filtration in suitable Filter Housings fitted with connectors other
than those required for use with the Pressure Elution Station. In this case, the Filter Module must
be transferred to a Filter Housing suitable for use with the Pressure Elution Station. For more
information, refer to “Eluting the Sample.”
Operating the Pressure Elution Station
Preparing the buffer solution
Before you begin mixing the buffer solution, determine the amount of solution required for the
number of samples you are going to elute. In addition, 400 mL of buffer solution is required for the
initial system check of the Pressure Elution Station and for each subsequent elution.
Phosphate buffered saline solution may be purchased in a ready-made solution or may be
prepared using individual ingredients as follows:
• Sodium chloride 8.0 g/L
• Potassium chloride 0.2 g/L
• Disodium hydrogen phosphate 1.15 g/L
• Potassium dihydrogen phosphate 0.2 g/L
To prepare 1 L of the buffer solution:
1. In a suitable container, add the individual ingredients for PBS to 1 L of reagent-grade water.
2. Place the container on a magnetic stirrer plate.
3. Add 0.1 mL of TWEEN 20, and mix for at least 10 minutes or until dissolved.
Note:
TWEEN 20 is very viscous and requires careful pipetting. Wipe the outside of the pipette
with a paper towel/tissue to ensure the correct amount is dispensed.
Sample flow
through Filter
Housing
QC body
Filter Module
Bolt end
Filter Housing lid
Filter Housing
base
QF stem
