ibidi Universal Fit 10918, Instruction Manual

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ibidi Heating System, Universal Fit
2 Intended Use
The ibidi Heating System, Universal Fit is a stage top incubator for live cell imaging that fits onto
inverted microscope stages with a universal mounting frame for multiwell plates. The geometry of
the chamber, with a heated glass plate above the sample, provides a platform for keeping microscopy
slides and dishes at a constant temperature during an experiment on a microscope.
For full control of the incubation environment, the ibidi Heating System, Universal Fit can be com-
bined with the ibidi Gas Incubation System to regulate the CO
2
and O
2
concentrations as well as the
humidity.
The modular concept of the Heating Inserts enables the use of a variety of microscopy labware rang-
ing from 35 mm dishes to regular slides.
3 Principle
Physiological Conditions in Live Cell Imaging
The Heated Lid and Heated Plate are designed to keep cells on-stage at 37°C in microscopy slides
and dishes (Figure
1 ). To achieve this, the glass top of the Heated Lid and the Heated Plate are
actively heated by the Temperature Controller. The Heated Lid prevents condensation effects inside
the incubation system (Figure
2 ).
Heated Plate
Heated Lid
40°C 37°
Gas mixture,
Humidity
Humidity Sensor
Humidity
O
2
Levels
CO
2
Levels
Temperature
Cell culture vessel
Figure 1 – Schematic cross-sectional view of the ibidi Heating System, Universal Fit.
Heated Plate
Heated Lid ibidi solution:
40 °C
37 °C
Heated Plate
Unheated Lid
34 °C
37 °C
Figure 2 – The Heated Lid prevents condensation effects on the lid of the cell culture vessel.
Version 2.2 (May 23, 2019) 14 Instruction Manual