Chapter IV. Troubleshooting the BAX® System
BAX® System X5 User Guide
57
Straight Line Signal
What It Looks Like
- The Raw data appears as
a negatively sloped line. The Processed data is
blank or shows system noise
Causes
– Straight line signal may be caused by
the following:
•
PCR tablet was accidentally removed
from the PCR tube before processing
(missing tablet)
•
Not enough sample lysate was added
to the PCR tubes, resulting in a dry
PCR tablet
•
Samples were run in “Detection only”
mo
de instead of “Run Full Process”
mode
•
Laboratory contaminants (such as
powder from gloves, detergents,
disinfectants, etc.) have interfered with
the PCR process
Solution
- Repeat the assay from last enrichment. Follow all
steps of the protocol exactly as described in this User Guide or
the assay’s package insert.
Troubleshooting Process Interruptions
In some cases, a full process run in the BAX® System X5 instrument may be interrupted before results are displayed
for such reasons as
•
unexpected power failure
•
data communication error
•
accidental cancellation of the process
In these cases, it may be possible to re-run the same PCR tubes in the instrument to generate results with the
Detection Only operation. This option allows you to re-analyze samples that have already begun the detection phase
of the standard full process. Results are available after about 1 hour.
Do not use the Detection Only process unless instructed to do so by Hygiena Diagnostics Support or your
local Hygiena representative or distributor.
Note: The Detection Only option SHOULD NOT be used to re-analyze samples for which results have already been
displayed.
Under the T
ools menu, select “Enable Detection
Only Run”.
Once selected, the
“Enable Detection Only Run”
option in the menu will be marked with a check.
Follow the instructions
under “Complete a BAX®
System Process Run” for the appropriate PCR
assay to pre-heat the instrument, load samples
and start processing.
