horiba HEMAPREP RAB316AEN, User Manual

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1. General Recommendations
1.1. Blood Sample Collection
Accepted types of blood
The system accepts both fresh blood and blood collected in anti-coagulant as EDTA.
We recommend you to perform operation as soon as possible after collection. EDTA can cause
morphological damages after four hours.
Capillary collection
If you use capillary tubes, we recommend you to use plain micro hematocrit tubes of approximately a
1 mm internal diameter.
If these tubes are filled at least halfway, they easily deliver a drop of blood.
Sticks collection
If you use sticks, it is more difficult to control the quantity of blood.
We recommend that you touch only one of the sticks to the slide to deliver the desired quantity of
blood.
If both sticks are touched at the same time, too much blood can be delivered.
1.2. Special Smears
Finger sticks
We do not recommend you to directly apply blood on the slides using fingers.
Using fingers increases the difficulty to manage the quantity of blood and correctly place the drop on
the slide.
We recommend you to use a 1 mm capillary tube to transfer the blood.
Make sure that the tube is filled at least halfway so that the blood can easily move.
Reticulocyte smears
We recommend you to keep the initial calibration settings and use a drop of blood half of the normal
quantity.
The system produces a slide with a monolayer over the major area.
You must clean the spreader blades after each specimen.
Workflow
General Recommendations
20 User Manual
Ref: RAB316AEN