manualshive.com logo in svg

Helena Laboratories ProtoFluor-Z 2005, Operator'S Manual

Share
Download
Helena Laboratories ProtoFluor-Z 2005 Operator'S Manual Download Page 28

ProtoFluor-Z                              TEN – Maintenance, Troubleshooting, and Warranty 

 
 

 

Page

 

24 

 

 

10.2  Troubleshooting 
 

If the recommended solutions should fail to solve a problem, call Helena Laboratories for 
assistance. 

Table 10-1.  Troubleshooting 

 
Problem 

 

 

 

Possible Cause 

 

Solution 

 

No power to instrument 

 

Power cord unplugged 

Plug cord into proper 

When power is turned 

 

 

 

 

 

wall outlet 

on 
 

 

 

 

 

Blown Fuse   

 

Replace fuse 

 

Light inside instrument 

 

Electrical problem   

Contact Helena 

But no readings 

 

 

 

 

 

 

Laboratories for 

 

 

 

 

 

 

 

 

 

assistance 

 

After zeroing, no readings   

Electrical problem   

Contact Helena 

 

 

 

 

 

 

 

 

 

 

Laboratories for 

 

 

 

 

 

 

 

 

 

assistance 

 

Err1, Err2, etc. 

 

 

Error found during   

Turn power off and on 

Displayed at power on 

 

self-test 

 

 

again.  If still is displayed, 

 

 

 

 

 

 

 

 

 

Call Helena Laboratories 

 

 

 

 

 

 

 

 

 

For assistance 

______________________________________________________________________ 
 

Display contains 

 

 

Error found during   

Turn power off and on 

Decimal points 

 

 

operation 

 

 

again.  If still is displayed, 

 

 

 

 

 

 

 

 

 

Call Helena Laboratories 

 

 

 

 

 

 

 

 

 

For assistance 

 

 

Instrument does not  

 

Electrical problem   

Contact Helena 

Respond to keys 

 

 

 

 

 

 

Laboratories for 

 

 

 

 

 

 

 

 

 

Assistance. 

Blanked coverslip value 

 

Dirty optics or 

 

Contact Helena 

Greater than 30 

 

 

dirty coverslips 

 

Laboratories for 

 

 

 

 

 

 

 

 

 

Assistance. 

Unable to calibrate   

 

Electrical problem   

Contact Helena 

 

 

 

 

 

 

 

 

 

Laboratories for 

 

 

 

 

 

 

 

 

 

Assistance.   

 

 

Unable to calibrate   

 

Instrument insufficiently 

Allow to warm longer 

 warmed 

Summary of Contents for ProtoFluor-Z 2005

Page 1: ...Helena Laboratories Operator s Manual ProtoFluor Z Hematofluorometer Cat No 2005 110 Vac Cat No 2006 220 Vac ...

Page 2: ...ProtoFluor Z Hematofluorometer Operator s Manual Cat No 2005 100 Vac Cat No 2006 220 Vac ...

Page 3: ...9 SEVEN Operating Instructions 11 7 1 Specimen Collection and Handling 11 7 2 Preparation and Warm up 12 7 3 Blank Instrument 12 7 4 Calibrate Instrument 13 7 5 Patient Sample Assay 14 7 6 Summary Instructions 15 7 7 Procedural Notes 18 7 8 Reference Values and Interpretation of Results 20 EIGHT Test Functions and Quality Contro 21 NINE Performance Specification 22 TEN Maintenance Troubleshooting ...

Page 4: ... Figures 1 1 ProtoFluor Z 1 1 2 1 Block Diagram 2 2 5 1 Front Panel Controls and Displays 5 1 5 2 Back Panel Controls 5 2 10 1 ProtoFluor Z Back 10 1 List of Tables 6 1 Inventory 6 1 10 1 Troubleshooting 10 2 ...

Page 5: ...amola and Yamane showed that the fluorescent erythrocyte and porphyrin associated with lead intoxication is zinc protoporphyrin ZPP and determined its absorption and fluorescence can be detected easily in whole blood by use of a front face fluorometer 6 7 The absorption 424 nm Soret band and fluorescence maxima 595 nm of ZPP in blood differ from those of the metal free porphyrins associated with v...

Page 6: ... variations in the intensity of the 415 nm light When the sample is exposed to 415 nm light zinc protoporphyrin ZPP is excited and emits light at 595 nm A second lens filter system collects filters and focuses the 595 nm light onto a photomultiplier tube PMT The PMT produces a current level in response to the light reaching it which is proportional to the ZPP H ratio In the five seconds after pres...

Page 7: ...ProtoFluor Z TWO Principles of Operation Page 3 Figure 2 1 Block Diagram Photocell ...

Page 8: ...use frozen specimens or specimens that have been stored longer than recommended 3 8 Specimens must be run with reagent Results with fresh samples may be significantly decreased when run without reagent and moderately aged samples run without reagent may generate unpredictable results 3 9 Abnormally elevated bilirubin will crea te positive interference due to its spect ral qualities 3 10 Riboflavin...

Page 9: ...e responsible body Under IEC 61010 2 101 2002 the person s responsible for the use and maintenance of equipment and for ensuring that operators and adequately trained for eliminating and reducing hazards involved in removal from use transportation or disposal 3 23 Action s to be taken in case of malfunction See section 3 6 and 10 2 3 24 Requirements for handling biohazards Due to potential biohaza...

Page 10: ...ucted to do so by a qualified service technician directly representing Helena Laboratories its subsidiaries or its distributors 4 2 Do not attempt to operate the instrument without plugging the power cord into an easily accessible grounded wall outlet of the proper voltage and frequency This information is contained on the serial number plate located on the back of the instrument 4 3 Before turnin...

Page 11: ...the instrument Voltage Switch Factory preset for 110 Vac or 220 Vac for domestic or foreign use Switch to Select Reporting Units Located on the back panel switch has three positions to select reporting units in umol ZPP mol Heme ug ZPP dL whole blood 25 hematocrit or ug ZPP dL whole blood 42 hematocrit 1 up ug dL using 42 Hct 2 middle umol mol 3 down ug dL using 35 Hct The mode selected is indicat...

Page 12: ...ProtoFluor Z FIVE Controls and Displays Page 8 Figure 5 2 Back Panel Controls ...

Page 13: ...embly for microhematocrit tube 6 2 Installation 1 Select an environment free of drafts excessive humidity dust and large temperature fluctuations 2 Place the unit on a level flat surface Make sure that there is enough space behind and around it to allow good air circulation 3 Insert the sample holder into the slit at the upper right marked SAMPLE The end with the hole should enter the instrument w...

Page 14: ...tions Page 10 The instrument s circuitry contains filters to reduce the effect of line voltage fluctuations however they should still be avoided If the operator experiences difficulty it may be necessary to install an isolation transformer ...

Page 15: ...color due to the rupture of red blood cell membranes Unreliable results Incorrectly low or high are obtained with hemolyzed specimens 2 Samples stored longer than recommended may have falsely increased or decreased results 3 Abnormally elevated bilirubin will create positive interference high values due to its spectral qualities Increased bilirubin is indicated by a bright yellow color in the plas...

Page 16: ...t Position Mode 1 Up ug dL 42 Hct 2 Mid umol mol 3 Down ug dL 35 Hct 4 Allow the instrument to warm up at least 30 minutes This 30 minute warm up warms the electronics in the instrument but the light bulb is on only when MEASURE is pressed 5 Press MEASURE 5 to 10 times to warm up the light bulb waiting approximately 5 seconds between each time the button is pressed for the screen to reset This cyc...

Page 17: ...ruments with a serial number beginning with 2 If the reading is outside this range obtain another coverslip insert it into the instrument and press BLANK again 6 Press MEASURE The coverslip should now measure 000 1 indicating that the unit is zeroed on the coverslip Note If 000 1 is not achieved repeat the process with a new coverslip If the new coverslip fails contact Helena Laboratories 7 4 Cali...

Page 18: ...in step 6 9 Withdraw the sample holder and discard the used coverslip Place a new coverslip in the sample holder insert it into the instrument and press BLANK 10 Remove the sample holder with the blanked coverslip from the instrument and place one drop of ProtoFluor Low Calibrator on it and insert it into the instrument 11 Press MEASURE The reading of the low calibrator should be within 3 6 for po...

Page 19: ...re running is not appropriate and not necessary due to the principle of front faced fluorometry 9 Record the result withdraw the sample holder and discard the coverslip 10 Prepare and measure the next sample and record the reading Multiple reagent sample preparations may be prepared at once as long as they are used with 5 minutes of preparation 7 6 Summary Instructions Note Before initial operatio...

Page 20: ...The reading should be 4 14 for instruments with serial number starting with 0 or 7 40 for instruments with serial number starting with 2 5 Press MEASURE The reading will be 000 1 if the instrument has properly blanked the coverslip Calibration 1 Remove the sample holder from the instrument and leave the coverslip in the sample holder 2 Place a drop of ProtoFluor High Calibrator in the center of th...

Page 21: ...on the coverslip Insert it in the instrument and press MEASURE The low calibrator should be within 3 6 for position 2 umol mol of the published value If the reading is not within this range press MEASURE 2 to 3 times If 2 out of 3 of the readings are within 3 6 for position 2 umol mol of the published value the instrument is properly calibrated If the readings are not satisfactory the instrument s...

Page 22: ...mination of the sample holder and the inside of the instrument with blood calibrators or control materials Clean the sample holder thoroughly if it is contaminated with a sample Contamination of the inside of the optical system will lead to erroneous results and the instrument must be taken apart and cleaned Be sure to contact the manufacturer prior to opening the instrument 2 Do not measure a sam...

Page 23: ...minate plasma interferences is not recommended 12 Freezing of specimens hemolysis failure to use reagent and failure to maintain instruments may cause falsely low results 13 When results are reported as a ratio of ZPP Heme results are not affected by hemoglobin concentration or hematocrit eliminating the need to correct for hematocrit 14 The instrument provides readings from 0 600 umol ZPP mol Hem...

Page 24: ...iosynthetic pathway of heme before anemia develops Elevated ZPP may also indicate anemia which may be associated with chronic infection or malignancy The presence of elevated blood lead levels also increased ZPP The CDC has lowered the recommended lead screening level in children from 25 jg dL to 10 ug dL Lead levels below 25 ug dL do not significantly affect ZPP formation and therefore the use of...

Page 25: ...y time the power is turned on The display should read PFZ Should an error message appear on the display turn the instrument off and on again Should the error message reappear see Section Ten Troubleshooting Each laboratory should establish its own normal range of expected values for the procedures in use Refer to Section 7 4 for the calibration procedure ...

Page 26: ... Watts 220 Vac 50 60 HZ 50 Watts Fuses 2 3 A 250 V slow blow 110 V unit 1 6 A 250 V slow blow 220 V unit Dimensions 7 1 2 in 19 cm High 11 5 8 in 29 5 cm Wide 11 1 4 in 28 6 cm Deep Weight 12 6 lb 5 7 kg Environment 15o to 30oC 59o to 86oF Performance Characteristics CV s of less than 5 for all within run run to tun and instrument to instrument precision tests ...

Page 27: ...ve Maintenance The ProtoFluor Z must have annual preventative maintenance including replacement of the optics Failure to do so may lead to decreased results Preventative maintenance must occur at Helena Laboratories A decrease in results may occur prior to detection by calibration and optical checks 10 1 3 Cleaning Spills Clean exterior spills with a soft cloth or sponge only after turning off the...

Page 28: ...sistance Err1 Err2 etc Error found during Turn power off and on Displayed at power on self test again If still is displayed Call Helena Laboratories For assistance ______________________________________________________________________ Display contains Error found during Turn power off and on Decimal points operation again If still is displayed Call Helena Laboratories For assistance Instrument doe...

Page 29: ...e affected component It does not cover any accessory hardware or software purchased by the operator to modify the unit from the original designed application 10 4 Bibliography 1 Trundle D S Erythrocyte Zinc Protoporphyrin Clin Chem News pg 8 August 1984 2 Labbe R F History and Background of Protoporphyrin Testing Clin Chem 23 2 256 259 1977 3 Labbe R F et al Erythrocyte Protoporphyrin Heme Ratio i...

Page 30: ...ProtoFluor Z ELEVEN Symbology Page 26 ELEVEN Symbology NOTE The following symbols may be used in this manual or on the instrument to provide information necessary to the user if applicable ...

Page 31: ...atofluorometer Operator s Manual Catalog No 2005 110 Vac Catalog No 2006 220 Vac For Additional Information or Assistance call your local distributor or call Helena Laboratories at 1 800 231 5663 toll free Helena Laboratories P O Box 752 Beaumont TX 77704 0752 USA ...

Reviews:

No comments

Brands by name

Popular brands

Load more brands