Helena Laboratories ProtoFluor-Z 2005, Operator'S Manual

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ProtoFluor-Z SEVEN – Operating Instructions
Page 13
a problem. Call Helena Electronics
Customer Services Department for
assistance.
The instrument requires 5 sec. to recover
from a reading. Therefore, it will not
accept a new command for 5 seconds
after a reading appears on the LED
display.
3. Place a clean lint-free coverslip into
the sample holder.
Handle coverslips with care to avoid lint,
fingerprints and scratches. Be sure
multiple coverslips are not stuck
together.
Any time a result is obtained that seems
out of line, such as duplicate samples
with very different readings, the first
suspect should be that 2 coverslips are
stuck together or that a coverslip was
dirty.
4. Insert the sample holder into the
instrument.
Be sure you feel the “click” indicating the
sample holder is completely inserted.
5. Press BLANK.
The number that appears on the LED
display is the value of the coverslip that
will be subtracted from the patient
reading. An acceptable value is 4-14 for
instruments with a serial number
beginning with 0, or 7-40 for instruments
with a serial number beginning with 2. If
the reading is outside this range, obtain
another coverslip, insert it into the
instrument, and press BLANK again.
6. Press MEASURE.
The coverslip should now measure 000
+/-1 indicating that the unit is zeroed on
the coverslip.
Note: If 000 +/- 1 is not achieved, repeat
the process with a new coverslip. If the
new coverslip fails, contact Helena
Laboratories.
7.4. Calibrate Instrument
1. Withdraw the sample holder from the
instrument.
2. Place a coverslip in the sample holder
and insert into the instrument.
Use the precautions noted in 7.3 for
correctly handling coverslips
3. Press BLANK.
4. Remove the sample holder with
blanked coverslip from the instrument
and place one drop of ProtoFluor High
Calibrator in the center of the coverslip.
The volume of the Calibrator on the
coverslip should be enough to make a
spot at least 8 to 10 mm in diameter in
the center of the coverslip. Covering the
entire opening in the sample holder is not
necessary.
Do not use a sample preparation
containing a bubble, since this will cause
falsely low readings.
5. Gently insert the sample holder into
the ProtoFluor Z and press MEASURE.
This initial reading of the calibrator may
be as much as +/-30 of the published
value.