Hach LANGE LICO 500 User Manual Download Page 80

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Advanced Operations

Remove any cuvettes/sample cells from the cell compartment
and press

Start

Insert the different filter in the given order one after the other.
Press

Next

after inserting a filter.

After the last measurement the results are displayed.

Press

PC & Printer

icon

to send the data to a USB memory

stick, PC or to a printer.

The files will be stored automatically as CSV file (Comma
Seperated Value). The file name will be formatted as
“Verification.csv“.

6.1.4 Output checks

If a printer is connected a test printing of the current screen will be
printed.

6.1.5 Lamp history

The Lamp History menu provides the amount of time that the lamp
has been on (Hours).

Summary of Contents for LANGE LICO 500

Page 2: ...2...

Page 3: ...rument Setup mode 19 5 2 1 Operator ID 19 5 2 2 Sample ID 20 5 2 3 Date and time 22 5 2 4 Display and sound preferences 22 5 2 5 Lamp control 22 5 2 6 PC and printer 24 5 2 6 1 Printer setup 24 5 2 6...

Page 4: ...6 3 Add a reference to the reference list 58 5 7 Photometry 59 5 7 1 Single Wavelength absorbance concentration and transmittance measurements 59 5 7 1 1 Set up single wavelength mode 60 5 7 1 2 Take...

Page 5: ...7 1 2 Display 85 7 1 3 Cuvettes sample cells 85 7 2 Lamp replacement 86 7 3 Filter pad maintenance 88 7 3 1 Filter pad replacement 88 Section 8 Troubleshooting 91 Section 9 Replacement Parts 93 9 1 R...

Page 6: ...4...

Page 7: ...range 3 5 Abs wavelength range 340 900 nm Photometric accuracy 5 m Abs at 0 0 to 0 5 Abs 1 at 0 50 to 2 0 Abs Photometric linearity 0 5 to 2 Abs 1 at 2 Abs with neutral glass at 546 nm Stray light 0...

Page 8: ...6...

Page 9: ...ry labels Read all labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A symbol if noted on the instrument will be included with a danger...

Page 10: ...0 nm The instrument comes with a complete set of application programs and multi language support The LICO 500 contains the following application modes Color scales pre installed Single Wavelength Mode...

Page 11: ...tion The relative humidity should be less than 80 moisture should not condense on the instrument Leave at least a 15 cm 6 in clearance at the top and on all sides for air circulation to avoid overheat...

Page 12: ...g the external power supply cord into the connector on the back panel of the instrument then plug the supply into a power outlet 100 240 V 50 60 Hz Press the power switch on the back of the instrument...

Page 13: ...B interface is used for communications with a PC The optional Hach Data Trans software see Section 9 on page 93 must be installed on the PC for this use A USB hub may be used to connect several acces...

Page 14: ...artment 1 insert the adapter Z into cell compartment 2 Cell compartment 2 Cell compartment 2 uses adapters to accommodate different cuvette sample cell types 50 mm rectangular cuvettes sample cells ca...

Page 15: ...ette sample cell 11 mm and or 10 mm square cuvette sample cell so the arrow on top of the adapter points to the left Figure 4 and the orientation tab fits the groove in the compartment opening Note Th...

Page 16: ...the LICO 500 Figure 5 Beam path 1 Tungsten lamp 9 Filter wheel 2 Entrance slit 10 Splitter mirror 3 Heat protection glass 11 Reference element 4 Grating 12 Cell compartment 2 5 LED 13 Lens 6 Chopper 1...

Page 17: ...The self check will start automatically Changing the language setting The instrument functions in the selected language until the option is changed 1 While turning the instrument on touch the screen a...

Page 18: ...a continuous operating time of more than 24 hour without switching off the instrument we recommend to perform a new calibration with a distilled water cuvette sample cell After a continuous operating...

Page 19: ...when programming the instrument Unavailable options are disabled grayed out The icons on the right and left of the screen are described in Table 1 The central keypad changes to reflect the selected en...

Page 20: ...ntration factor can be entered to enable the measured absorbance values to be converted into concentration values MULTI WAVELENGTH In the multi wavelength mode absorbance Abs or percentage transmittan...

Page 21: ...perator ID This feature helps record which operator measured each sample 1 Press Operator ID in the Instrument Setup 2 Press New to enter a new Operator ID 3 Use the alphanumeric keypad to enter a new...

Page 22: ...splay shows the selected Operator ID 11 Press OK to return to Instrument Setup 12 The selected Operator ID is activated Note Press Delete to remove an Operator ID from the list Note Alternatively ente...

Page 23: ...number of the sequence Use the key between the arrow keys to enter the first number of the sequence using the alphanumeric keypad 6 Press OK to return to Instrument Setup 7 The Sample ID is activated...

Page 24: ...Default short beep every time a reading is complete Timer Adjusts the length of the timer sound Select Short or Long Long beeps are recommended for noisy environments 2 Select Long to change the numb...

Page 25: ...on the Lamp 3 Select Save in order to define a time interval for the burning time of the lamp 4 Press the field below Save to select the lamp burning time 5 Select the length of time the lamp will be...

Page 26: ...sories at a time A USB memory stick is used to upgrade data see section 6 1 2 on page 76 Important Note A screened USB cable must not be longer than 3 m 1 Press PC Printer in the Instrument Setup A li...

Page 27: ...f is available 3 Select Auto Send On to send all measured data automatically to the Thermal printer Note The option Auto Send is not available for any other printer e g ink jet printer 4 Press Resolut...

Page 28: ...K again to return to the Instrument Setup menu 5 2 6 2 PC setup 1 Press PC 2 Press Setup to display the PC Setup screen 3 With Auto Send ON selection each measurement result will be automaticly sent t...

Page 29: ...printed are stored 3 A list is displayed Data can be filtered For more information see 5 3 1 2 Recall stored data from the color log or the data log on page 31 4 Press the Printer icon 5 Highlight Si...

Page 30: ...d programs or instrument configurations 1 Press Password in the Instrument Setup menu 2 In order to highlight the Security List assign a password Press Set Password 3 Use the alphanumeric keypad to en...

Page 31: ...rn to Instrument Setup Note The alphanumeric keypad to the Password inquiry appears when a user tries to reach a locked setting 5 2 7 1 Deactivate a password 1 Press Password in the Instrument Setup 2...

Page 32: ...ct Color menu 1 Press Select color in the Instrument Setup A color chart list will appear 2 Select a color category to highlight the color for the display background 3 Press OK to return to Instrument...

Page 33: ...elete data from the color log and data log 5 3 1 1 Auto manual data storage The data storage parameter indicates whether data are to be stored automatically or manually in which case the user has to d...

Page 34: ...Scale 9 Press Options and Graph Data 10 A graphic trend of all color values stored in the selected period and color system is displayed The trend shows a visual impression of the measured value proce...

Page 35: ...e over 5 3 1 3 Send data from the color log or the data log Data is sent from the data log as CSV Comma Separated Value files through a USB memory stick to a file named DATALOG The file can then be pr...

Page 36: ...C The optional HACH Data Trans software must be installed on the PC for the subsequent to process for measurement data 1 Press PC Printer in the Instrument Setup 2 Select PC 3 Press Setup to display t...

Page 37: ...r the data log 1 Press Recall Data in the Main Menu 2 Press Color Log or Data Log then Options Delete 3 Highlight Single Point or Filtered data or All data and press OK to confirm Note The number in p...

Page 38: ...torage from wavelength scan or time course 1 Press Options then the Store icon after a reading is taken The Store Data list will be displayed 2 Press Store to save the current scan to the highlighted...

Page 39: ...to the Recall Data list 5 3 2 3 Send data from wavelength scan or time course There are two ways to recall sent data to a USB memory stick printer or PC with Hach Data Trans Option1 1 Press Recall Da...

Page 40: ...te appears when the files were already stored Press OK to overwrite the stored data Option 2 1 Press Wavelength Scan or Time Course and then Options More Send Data to send the data to a USB memory sti...

Page 41: ...gth scan or time course 1 Press Recall Data from the Main Menu and then Wavelength Scan or Time Course or Options More Recall Data A listing of the stored data is displayed 2 Highlight any data to del...

Page 42: ...ilable for the 11 mm disposable round glass cuvettes sample cells The dry thermostat heats the cuvettes sample cells to any temperature between ambiant and 150 C Important Note The samples must be cle...

Page 43: ...on page 40 for more sample and sampling preparation information The Color Measurement mode is used to determine absolute color values in the Hazen Gardner CIE Lab or European Pharmacopoeia ranges The...

Page 44: ...results to be obtained 3 The calibration starts when the LICO 500 automatically recognizes the cuvette sample cell The type of cuvette sample cell and the actual progress of calibration is shown in a...

Page 45: ...ere the user has immediate access to special software options Figure 7 Touch sensitive areas on the measurement window 1 Open Operator ID to change or add operator ID see section 5 2 1 on page 19 5 Ch...

Page 46: ...of the transmission or absorbance curve Note View Graph is activated after the first reading VIEW TABLE shows the spectral transmission values T from 380 nm to 720 nm VIEW VALUES show the result of t...

Page 47: ...ent 1 Press the touch sensitive area 3 e g Color Value Hazen 2 A list of all color scales is displayed 3 Select the required scale 4 Press OK to confirm 5 The current result of the actual reading and...

Page 48: ...h the Ph Eur system Automatic The color of the solution is compared to the colors of the 37 color reference solutions The identifier of the reference solution whose color is closest to that of the sam...

Page 49: ...8 5 Select Auto 6 Press Graph The additional color matching result of the Pharm Eur Color is displayed The upper value shows the actual option for the color matching calculation which can be Auto for...

Page 50: ...is selected the a b color locus of the measured sample will be displayed in all 5 color scales of the Pharm Eur 12 Press Print to print the graph The CIE L a b values depend on the path length of the...

Page 51: ...earest will appear followed by the name of the reference solution with the lowest color difference E In this case select Auto for a color matching in all scales to see which color scale and color refe...

Page 52: ...measurement The CIE L a b values depend on the path length and are always related to the type of cuvette sample cell used 1 Select US Pharmacopeia 2 Insert the sample cuvette cell 3 The result of the...

Page 53: ...e to cuvette sample cell tolerances 5 5 6 Determine the Gardner color value The Gardner color value is defined in DIN ISO 4630 The lighter Gardner color values 1 to 8 are based on potassium chloroplat...

Page 54: ...ded 5 5 10 AOCS Cc 13 e Lovibond scale The calculation of the Lovibond color values in the LICO 500 is derived from the AOCS Cc 13e or BS 684 1 14 method and is based on the 5 1 2 inch or 1 inch yello...

Page 55: ...ican Dye Manufacturers Institute ADMI has adopted the Platinum Cobalt standard of the American Public Health Association APHA as the standard for color value Although this standard is yellow the ADMI...

Page 56: ...e memory for up to 50 references is available 5 6 1 Take a color difference measurement 1 From the Main Menu press Color Difference Measurement 2 Insert a reference cuvette cell 3 The CIE Lab Referenc...

Page 57: ...absorbance curve View Graph will be activated after the first reading VIEW TABLE show the spectral transmission values T from 380nm to 720nm VIEW VALUES show the result of the last color calculation...

Page 58: ...ton on the right screen to move the cursor line left or right or touch any position inside of the graph area In the middle of the x axis the wavelength at the actual cursor position is displayed 3 Pre...

Page 59: ...e Main Menu 2 Press Select Reference 3 A list of available references is displayed 4 Press the required reference 5 Press Select reference to confirm 6 Insert the sample cuvette cell Important Note Fo...

Page 60: ...fference measurment with 50 mm cuvette sample cell 5 6 3 Add a reference to the reference list 1 Press Color Difference Measurement from the Main Menu 2 Insert a reference cuvette cell 3 Press Options...

Page 61: ...ments at a single wavelength the instrument can be programmed to measure the absorbance transmittance or concentration of the analyte Absorbance measures the amount of light absorbed by the sample in...

Page 62: ...entered wavelength must be in the range from 320 1100 nm Send Data To send data to a printer PC or USB memory stick Concentration Factor Multiplication factor for converting absorbance values into co...

Page 63: ...dings are to be multiplied Press the Unit key to select the units for concentration measurements or to create a new unit 3 Press OK to confirm Concentration resolution 1 Press Concentration Resolution...

Page 64: ...storage see section 5 3 1 on page 31 5 7 2 Multi Wavelength mode measurements at more than one wavelength In the multi wavelength mode absorbance values can be measured at up to four wavelengths and...

Page 65: ...urement data are stored automatically With the Store Off setting no measurement data are stored Trans Abs To switch to transmittance concentration or absorbance readings Wavelength To enter the measur...

Page 66: ...press one of the KX keys Enter the desired coefficient into the numeric keypad Press OK to confirm Note The instrument allows entry of up to 5 significant digits with a maximum of 4 significant digits...

Page 67: ...trums In the wavelength scan mode the absorbance of the light in a solution over a defined wavelength spectrum is measured The measurement results can be displayed as a curve as percentage transmittan...

Page 68: ...r to switch the display back and forth between the scan data tables wavelength absorbance and the graph of the curve Note Select View will be activated after the first reading Cursor Mode To select Tr...

Page 69: ...olution data Selecting a larger step allows the instrument to scan faster but decreases the resolution of the collected data 5 According to the selected wavelength range select the interval from the a...

Page 70: ...mode Note The Integral is shown instead of the date on the display Note For the next scan measurement the setting for the Integral will be On Scale units 1 Press Scale Units 2 Highlight the required u...

Page 71: ...of the absorbance or transmittance values at the scanned wavelengths is displayed continuously The Wavelength Scan is complete if the graph is shown full size the scaling of the x axis fits automatica...

Page 72: ...lack one relates to the current wavelength scan 3 To complete the wavelength scan reading see section 5 7 3 2 on page 68 The newly plotted wavelength scan curves and the absorbance or transmittance va...

Page 73: ...curve is shown in orange The absorbance or transmittance value and the associated wavelength are highlighted in orange Additionally the difference of the extinction and or transmission value between...

Page 74: ...for data collection and the time interval between the collection of data points To input the wavelength setting View Table To display readings in absorbance transmittance or concentration This can be...

Page 75: ...2 Time course scan reading After the parameters have been selected the instrument must be blanked then the sample can be analyzed 1 Insert the blank cuvette cell into the cuvette sample cell holder Pr...

Page 76: ...mode A second cursor is highlighted The position of the fixed cursor was previously defined in Cursor Mode Single Use the active cursor to select any point on the measurement curve The difference to...

Page 77: ...stem Checks in the Main Menu The System Checks menu contains instrument information and various performance tests 6 1 1 Instrument information 1 Press Instrument Information in the System Checks menu...

Page 78: ...the software is updated 9 Press OK to return to the System Checks menu Note When the instrument software has been updated a prompt to restart the instrument is displayed 6 1 3 Optical checks 1 Press...

Page 79: ...gth nominal values and tolerances given in the quality control certificate to the following specifications Stray Light Photometrical accuracy Wavelength accuracy 3 Press OK when all values are entered...

Page 80: ...he results are displayed 8 Press PC Printer icon to send the data to a USB memory stick PC or to a printer The files will be stored automatically as CSV file Comma Seperated Value The file name will b...

Page 81: ...actory Service menu is password protected This menu is not intended for customer use 6 1 6 Service time In order to make sure a regular inspection an automatic memory reference for the service times c...

Page 82: ...d of time up to the next inspection 5 Press OK to confirm If the next service is due the message Next service is due is displayed after switching on the instrument 6 Press OK to return to the Main Men...

Page 83: ...s Instrument Backup in the System Checks menu 2 Connect the USB memory stick section 3 4 on page 11 3 Press Store to start a Backup Note If the USB stick is not connected the message Please insert USB...

Page 84: ...rent data will be overwritten when restoring the Backup file 1 Press Instrument Backup in the System Checks menu 2 Connect the USB memory stick containing the Backup section 3 4 on page 11 3 Press Res...

Page 85: ...83 Advanced Operations 4 Press OK to confirm after the message Instrument Backup from S N XXXXXXX Restore is displayed 5 After the backup start the instrument again...

Page 86: ...84...

Page 87: ...ents Do not insert a brush or sharp object into Cell Compartment 1 to avoid damaging the mechanical components Dry the cleaned parts carefully with a soft cotton cloth 7 1 2 Display Take care not to s...

Page 88: ...g the lamp 1 Switch the instrument off 2 Unplug the power cord WARNING Burn Hazard Wait until the lamp cools down Contact with the hot lamp can cause burns 3 Use a screwdriver to remove the cover from...

Page 89: ...ces on the skin can bake onto the lamp bulb and thus accelerate the ageing process of the lamp 8 Plug a new halogen lamp to the panel 9 Insert the halogen lamp with the half rounded part pointing up 1...

Page 90: ...sample cells and cell adapter from the cell compartment 2 Turn the instrument off 3 Unplug the power cord 4 Lift the instrument and check the color of the filter pad Replace the filter pad if is dark...

Page 91: ...r pad Figure 8 item 3 and replace it with a new one 9 Screw the grid back in place 10 Carefully stand the instrument upright 11 Plug the instrument in Figure 8 Base of LICO 500 with filter grid 1 Filt...

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Page 93: ...d Self Check Test stops while starting the instrument Remove the cuvette sample cell from the cell compartment Press OK Error Selfcheck stopped Hardware error Error x Electronic defect Contact the man...

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Page 95: ...with nominal values LZM339 Dust cover HYH020 Filter pad A23766 Thermo printer PD 24 power line EU USB cable 1 roll of paper 5835900 00 Thermo printer PD 24 power line UK USB cable 1 roll of paper 5835...

Page 96: ...94...

Page 97: ...Fax 39 02 39 23 14 39 info hach lange it www hach lange it HACH LANGE S L U Edif Arteaga Centrum C Larrauri 1C 2 Pl E 48160 Derio Vizcaya Tel 34 94 657 33 88 Fax 34 94 657 33 97 info hach lange es www...

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Page 99: ...gn poor materials or inadequate finish will be improved or replaced at the supplier s discretion The identification of such defects must be notified to the supplier in writing without delay however at...

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Page 101: ...1 82 Instrument Information 75 Instrument Setup 18 19 60 63 66 72 Interfaces 11 24 Internet 76 Iodine color value 50 K Klett color number 51 L Lamp see VIS Lamp Language 15 Liability 97 Lovibond scale...

Page 102: ...h Screen 17 Touch sensitive area 43 Troubleshooting 91 U Unpacking the instrument 9 Update 76 V Verification Kit 76 View Table 72 VIS Lamp Lamp Control Lamp History 78 W Warranty 97 Wavelength 59 60 6...

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