59
Standard Operations
5.7 Photometry
In the photometry mode, photometric measurements can be carried
out as:
•
Single wavelength measurements
•
Multi wavelength measurements
•
Time course
•
Wavelength Scans
5.7.1 Single Wavelength (absorbance, concentration and transmittance measurements)
The Single Wavelength mode can be used in three ways. For
sample measurements at a single wavelength, the instrument can
be programmed to measure the absorbance, % transmittance or
concentration of the analyte.
Absorbance measures the amount of light absorbed by the sample,
in units of Absorbance.
% transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turn the concentration factor on to select a specific multiplier for
converting absorbance readings to concentration. In a graph of
concentration versus the absorbance, the concentration factor is
the slope of the line.
