Greiner Bio-One GmbH
Frickenhausen, Germany
PHONE
+49 7022 948-0 /
FAX
+49 7022 948-514 /
IFU F076056 EN
[rev.00, 07.2021]
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Instructions to transfer 3D cell cultures using the 24 Well Multi-MagPen
To assure sterility clean the Multi-MagPen Drive with 70 % ethanol. DO NOT autoclave the Multi-
MagPen
Drive (see also safety precautions). During the cellular transfer process, the
Multi-MegPen Drive is
inserted into the sterile Multi-MagPen Sleeve. Hence the Multi-MagPen Drive is never in direct contact
with the cell culture itself.
If media must be removed from the
wells, the microplate should be placed
on the 24 Well Holding Drive (part of
the 24 Well Bio-Assembler Kit; Item
No. 662840) to secure the 3D cell
cultures at the bottom of the wells
during pipetting.
Prepare Receiver Plate
The receiver plate should contain the same liquid
volume as the donor plate (1200 - 1500 µl/well). If
the media volume is too low, the Multi-MagPen
Sleeve will not touch or dip in the liquid and 3D cell
cultures will not be transferred properly to the
new plate. If the media volume is too high, media
will spill out the well as soon as the Multi-MagPen
Sleeve will dip in and 3D cell cultures might get lost.
Prepare Donor Plate
To assure liquid contact and reliable attachment
of the 3D cell cultures to the Multi-MagPen Sleeve
the recommended working volume per well is
1200 – 1500 μl
. If the media volume is too low, the
Multi-MagPen Sleeve will not touch or dip in the
liquid and 3D cell cultures will not attach to it. If the
media volume is too high, media will spill out the
well as soon as the Multi-MagPen Sleeve will dip in.
3/ INSTRUCTIONS
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