Personal bioreactor RTS-1 & RTS-1C
Edition 3.-4.03
– July 2017
Operating instructions
Page 7
5. Calibration
5.1.
Checking hardware version.
To check the hardware version, press
and
keys (fig. 2/2) simultaneously. Display will show a
new screen with a unique fourteen-digit unit ID (fig. 3/1) and the hardware version (fig. 3/2). Please refer to the number in the
version for further calibration. Display will change back to the previous screen after 4 seconds.
5.2.
Calibration verification.
The device is software calibrated (hardware version 9 or later) at the factory for specific microorganism
size of 0.4-0.8 x 1-
3 μm and a cell volume of approximately 0.5-5.0 μm
3
for operation with TPP TubeSpin
®
Bioreactor 50ml tube
at temperature range from +4°C to +70°C.
Figure 3. ID and hardware version
To verify the conformity of calibration follow the subsequent procedures:
-
Take a TPP TubeSpin
®
Bioreactor 50ml tube;
-
Add 10ml (± 0.05ml) distilled water;
-
Close the cap of the tube thoroughly;
-
Insert the tube into the socket (fig. 1/4);
-
Connect the device to the computer, launch the software and select factory calibration;
Note!
The software calibration works only when the device is connected to the software and appropriate factory calibra-
tion is selected in the settings.
-
Set the volume parameter of the distilled water in the software;
-
Set the measurement frequency to 1 minute;
-
Press the
Play
button in the software;
-
The device will start measuring in 1 minute and should complete after 15-20 seconds and OD value should appear on the dis-
play;
-
If OD value equals 0 (±0.1 OD) then the device corresponds to factory pre-calibration settings and is suitable for use.
5.3.
Creating user calibration
Note!
The device software calibration is a feature that will work for devices with hardware version 9 or later. Devices
with earlier versions come pre-calibrated with a possibility to reset baseline value for device maintenance.
5.3.1. Get cell suspension samples in falcon tubes with typical optical densities of your experiments. If the maximal OD of your experi-
ment (stationary phase) is 5 OD
600nm
then the recommended samples are 0 (ddH
2
O water or broth media) 1, 2, 3, 4, 5, 6 OD
600nm
.
Volume accuracy of the samples must be
±0.05.
Measure OD at desired wavelength of each cell suspension using a spectrophotometer with proper prior dilutions. The propor-
tionality between OD and cell density exists only for OD ≤ 0.4 (approximately), we recommend diluting samples to the range of
0.1-0.2 OD.
Multiply the dilution factor values to get the OD of the samples.
Continue to software manual page
29
.
5.3.2. RTS-1 / RTS-1C can be calibrated to detect scattered light of any possible cell with any possible shape and size, but due to
difference of light scattering in various cell suspensions, we cannot guarantee the stated measurement range in all conditions.
5.4.
Calibration verification for hardware versions 8 and earlier.
The device is hardware calibrated at the factory for specific microorganism size of 0.4-0.8 x 1-
3 μm and a cell volume of approxi-
mately 0.5-5.
0 μm
3
for operation with 50
ml tube at temperature range from +4°C to +70°C and saves calibration data when being
switched off. To verify the conformity of calibration follow the subsequent procedures.
-
Take a TPP TubeSpin
®
Bioreactor 50 ml tube;
-
Add 10ml (± 0.05ml) distilled water;
-
Close the cap of the tube thoroughly;
-
Insert the tube into the socket (fig. 1/4);
-
Set the volume parameter of the distilled water on the display (fig. 2/11);
-
Press
Run Stop
key (fig. 2/9) (the device will start the OD measurement cycle by accelerating to 2000 rpm);
-
The measurement cycle should complete after 15-20 seconds and OD value should appear on the display;
-
If OD value equals 0
±0.1 OD, then the device corresponds to factory pre-calibration settings and is suitable for use.
If the result is unsatisfactory, please follow instructions from section
Device calibration for maintenance
.