Edition 3-4.03
– July 2017
Personal bioreactor RTS-1 & RTS-1C
Page 6
Operating instructions
4. Getting started
4.1.
Unpacking
Remove packing materials carefully and retain them for future shipment and storage of the unit. Examine the unit carefully for any
damage incurred during transit. The warranty does not cover in-transit damage.
4.2.
Complete set. The unit set includes:
-
RTS-1 / RTS-1C, Personal Bioreactor................................................................................................................................... 1 pce
-
Lid ......................................................................................................................................................................................... 1 pce
-
Bioreactor vessels TPP TubeSpin
®
Bioreactor 50ml ........................................................................................................... 20 pcs
-
USB data cable ..................................................................................................................................................................... 1 pce
-
USB disk drive with software installation files ........................................................................................................................ 1 pce
-
External power supply ........................................................................................................................................................... 1 pce
-
Software installation and operating manual ......................................................................................................................... 1 copy
-
Operating Manual, Certificate .............................................................................................................................................. 1 copy
Figure 1. Rear panel
Figure 2. Control panel
4.3.
Setup
-
Place the unit on even, horizontal working surface;
-
Connect the external power supply to the socket (fig. 1/1) on the rear side of the unit;
-
Switch on the computer, if it was turned off;
-
Connect the USB data cable to the unit (fig. 1/2) and to the personal computer;
-
Insert the USB disk drive in the personal computer and install the software following the software installation procedure de-
scribed in software installation manual.
4.4.
Bioreactor vessel features:
-
Falcon type tubes. TPP TubeSpin
®
Bioreactor;
-
Working volume 10
– 30 ml;
-
Conical form;
-
5 openings (A, B, C, D, E) of different size above the gas permeable, sterile PTFE filter of the screw cap;
-
Openings can be sealed and by this, exchange adjusted to need;
-
Sterile gas exchange is gu
aranteed by the 0.22 µm filter membrane;
-
Even with a high cell density the supply of oxygen through the openings is sufficient;
-
Tube fits in a standard 50 ml centrifuge rotor.
4.5.
Due to the specificity of mould type manufacturing of centrifugal falcon tubes, the helical structure of the screw caps screw thread
can vary, and, given the vigorous mixing conditions, the liquid can spill if the tube is not closed tightly. Unfortunately, some of the
tubes can be faulty and the liquid spillage is inevitable in at least 1 out of 60 tubes.
Before launching the experiment and leaving the device, tubes must be checked for liquid spillage occurring in a period of at least
2 minutes at 2000 RPM and 1 s
-1
Reverse Spin (RS) with a closed lid. If droplets of liquid will appear on the inner surface of the
lid, then the screw cap is faulty and the tube must be replaced.