DISPENSING
Place a 96-well microplate at the front location of the left drawer
and keep it fully
retracted in the rear position.
Move the pipetting head
to the left and
align the tips with the microplate.
•
Pull the pipetting head slowly down
into the wells , but do not touch the
bottom of the wells.
Squeeze the pipetting handle
slowly to
dispense all the solution.
•
Pull the pipetting head slowly up with
the pipetting handle fully squeezed.
•
Gently retract the pipetting handle.
To simplify this operation, a height adjuster
4
can be set by turning the upper screw
so that the pipetting head may only be
moved down to a position slightly above
the bottom.
Pipetting 384 wells
PLATEMASTER is able to load 384-well plates through four movements using the
384-well plate adapter.
STEP 1:
•
Place a 384-well microplate on the accessory and make sure that it is located at
the first step by checking the position on the blue wheel.
•
Aspirate the solution.
•
Move the pipetting head over the microplate and dispense the first 96 wells.
STEP 2:
•
Move the microplate on to the second position of the 384-well plate adapter
using the blue wheel.
•
Aspirate the solution. Move the pipetting head over the microplate and
dispense the next 96 wells.
Figure 10
384-well adapter system
STEP 3:
•
Move the microplate on to the third position of the 384-well plate adapter
using the blue wheel.
•
Aspirate the solution. Move the pipetting head over the microplate and
dispense 96 wells.
STEP 4:
•
Move the microplate on to the fourth position of the 384-well plates adapter
using the blue wheel.
•
Aspirate the solution. Move the pipetting head over the microplate and
dispense the last 96 wells.
The 384-well microplate is loaded in less than one minute requiring only four liquid
aspiration steps.
Tip Ejection
•
Move the pipetting head over the empty rack on the right drawer.
•
Pull the pipetting head down to put the used tips into the original rack (tip rack
will be reused as a receptacle for tip waste).
•
Squeeze the tip-ejector
at the top of the pipetting head to eject the tips into
the rack.
Figure 11
Tip ejection system
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11
10
PLATEMASTER®
|
USER’S GUIDE
OPERATION
|
PLATEMASTER®