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8

7- settIng the voLume

The  volume  of  liquid  to  be  aspirated  is  set  using  the 

volumeter.  The  volumeter  consists  of  three  number-

dials, which are read from top (most significant digit) to 

bottom (least significant digit). a marker is used to set 

exact or intermediate volumes using the scale on the 

bottom dial. The dials are colored either black or red to 

indicate the position of the decimal point, according to 

the model (see examples).

Model 

Color of volumeter numbers

 

Black 

Red

M10, M25, M50 

µL 

0.1 µL

M100, M250 

µL 

none

M1000 

0.1 and 0.01 mL 

mL

The volume is set by turning the thumbwheel slowly to 

reach the required setting.

To obtain maximum accuracy when setting the volume, 

proceed as follows:
-  when 

decreasing 

the  volume 

setting, slowly reach the required 

setting, making sure not to over-

shoot the mark.

-  when 

increasing  the  volume 

setting, pass the required value 

by 1/3 of a turn and then slowly 

decrease  the  volume  to  reach 

the  required  setting,  making 

sure not to overshoot the mark.

0

6

8

6.8 µL

M10

0

6

8

68 µL

M100

8

3

6

8

36.8 µL

M50

0

6

6.8 µL

M25

1

6

8

168 µL

M250

0

7

5

0.75 mL

M1000

Summary of Contents for Microman M10

Page 1: ...The pipette of Choice for Problem Liquids ...

Page 2: ... 5 using microman 5 6 fittingthe capillary and piston 6 7 settingthevolume 8 8 pipetting 9 9 GLP features 10 10 troubleshooting 11 11 cleaning and decontamination 11 12 specifications 14 WARRANTY 16 EC DECLARATION OF CONFORMITY 17 Before use please read carefully the Warranty page 16 of this User s Guide ...

Page 3: ...roman pipettes are equipped with a positive displacement mechanism that isolates the aspirated liquid from the body of the pipette The positive dis placementmechanismpreventsthesample to sample contamination that can result from the aerosol effect Microman uses disposable capillaries and pistons Changing these parts which must be the only ones to make contact with the aspirated liquids can pro vid...

Page 4: ...resent Microman User s Guide Safety bag Adhesive id tags strip of 6 Capillary pistons 10 Certificate of conformity including bar code sticker 3 Description 1 Colorcodedpush button red forautoclavableMicroman and magnifier with volume range for aspirating and dispensing 2 Body or handle 3 Capillary 4 Piston 5 Capillary piston holder 6 Shaft 7 Thumbwheel for setting the volume 8 Volumeter 1 7 8 2 6 ...

Page 5: ...Polypropylene Polyester CP25 M50 SS Be Alloy Polyester PVDF Polypropylene Polyester CP50 M100 SS Be Alloy Polyester PVDF Polypropylene Polyethylene CP100 M250 SS Be Alloy Polyester PVDF Polypropylene Polyethylene CP250 M1000 SS Be Alloy Polyester PVDF Polypropylene Polyacetal CP1000 SS Stainless Steel Be Beryllium PVDF Polyvinylidene Fluoride 5 using microman Three simple steps are necessary 1 Mou...

Page 6: ...his procedure 1 Takecarenottodamagethesealingtip1whenyou handle the piston 3 2 Press the push button to the second stop to open the clamp 5 6 5 Microman CP model CP10 M10 CP25 M25 CP50 M50 CP100 M100 CP250 M250 CP1000 M1000 2 3 4 1 Never lubricate the capillary holder capillary or any other part of the pipette If the capillary tends to slip off the capillary holder clean the capillary holderwithet...

Page 7: ...ond stop po sition The piston clamp should now be open and protruding from the capillary holder 2 Slide the Microman piston clampinsidethetopofthecapil larytubeuntilthepistonclampis halfwaydownthepistonmount ing stem 3 Slowly release the push button while pushing the body of the Microman into the capillary piston until it is firmly seated on the Microman 4 Continue releasing the push button to ens...

Page 8: ...olorofvolumeternumbers Black Red M10 M25 M50 µL 0 1 µL M100 M250 µL none M1000 0 1 and 0 01 mL mL The volume is set by turning the thumbwheel slowly to reach the required setting To obtain maximum accuracy when setting the volume proceed as follows when decreasing the volume setting slowly reach the required setting making sure not to over shoot the mark when increasing the volume setting passther...

Page 9: ...op position wipeanyliquidfromtheout side of the capillary taking carenottotouchtheorifice Dispensing place the end of the capillary against the inside wall of the recipient vessel press the push button slowly to the first stop keepingthepush buttondepressed movethecapillary away from the side wall withdrawMicromanfromthevesselandreleasethe push button Ejecting the Piston capillary Press the push b...

Page 10: ...nformity according to ISO8655 Microman and CP are CE IVD labeled 10 troubleshooting You may be able to identify and to correct the problem by reference to the following table If you can t solve the problem contact your Gilson representative Leaks Change the capillary and piston No stroke The piston is not properly fitted into the clamp check that the piston is seated correctly refer to pages 6 7 I...

Page 11: ...ng and decontamination Microman is designed so that the parts normally in contact with liquid contaminants can easily be cleaned and decontaminated Cleaning The pipette must be cleaned with soap solution before it is decontaminated External 1 Wipe the entire pipette with a soft cloth or lint free tissueimpregnatedwithsoapsolution toremoveall dirty marks If the pipette is very dirty a brush with so...

Page 12: ...ble the pipette Decontamination You may refer to the Decontamination Procedure LT802288 available at www gilson com Chemical Decontamination The pipette should be cleaned before it is decontami nated Full details of recommended decontamination procedures for Gilson pipettes are available from your supplier Whatever other decontaminant you use check with the supplier of the decontaminant that it is...

Page 13: ...he individual components with distilled or sterilized water 6 Leave the parts to dry by evaporation or wipe them with a clean lint free tissue or a soft cloth 7 Reassemble the pipette Autoclaving Only Microman M10 and M100 are autoclavable The four other models Microman M25 M50 M250 and M1000 are not autoclavable 1 Unscrew the capillary holder shaft 2 Removetheclampassemblyandreturnspringfrom the ...

Page 14: ...O 8655 6 Basis of adjustment Ex Reference temperature 20 C Relative humidity 50 Barometric pressure 101 kPa Use of distilled water grade 3 ISO 3696 Ten measurements for each test volume which are Nominal Volume 50 of Nominal Volume and the minimum or 10 of Nominal Volume Performance Tests Eachpipetteisinspectedandvalidatedaccordingtothe Gilson Quality Assurance System Based on extensive historical...

Page 15: ...0 1 F148501 5 0 10 0 03 0 2 0 1 Max 10 0 15 0 06 0 2 0 1 M25 Min 3 0 25 0 08 0 12 0 3 F148502 10 0 27 0 08 0 12 0 3 Max 25 0 30 0 10 0 12 0 3 M50 Min 20 0 34 0 20 0 20 0 3 F148503 Max 50 0 70 0 30 0 20 0 3 M100 Min 10 0 50 0 20 0 80 0 6 F148504 50 0 75 0 30 0 80 0 6 Max 100 1 00 0 40 0 80 0 6 M250 Min 50 1 50 0 30 8 3 0 F148505 100 1 70 0 30 8 3 0 Max 250 2 50 0 50 8 3 0 M1000 Min 100 3 0 1 6 12 4...

Page 16: ...arranty shall also be void in theeventpipettesarealteredormodifiedbyanypartyotherthan Gilson or its designates Gilson s sole liability under this warranty shallbelimitedto atGilson ssoleoption repairorreplacementof any defective components of pipettes or refund of the purchase price paid for such pipettes THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES WHETHER ...

Page 17: ...rements of the following European Directives Villiers le Bel November 18th 2011 A El Sayed H Ledorze General Manager Quality Manager GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 www gilson com Annex III self declared The company Hereby certifies on its sole responsibility that the products listed below ...

Page 18: ...18 NOTES ...

Page 19: ...19 ...

Page 20: ... Gilson Inc World Headquarters 3000 Parmenter Street I P O Box 620027 I Middleton WI 53562 0027 USA Tel 1 800 445 7661 or 1 608 836 1551 I Fax 1 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs I BP 145 F 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 I Fax 33 0 1 34 29 50 20 www gilson com sales gilson com service gilson com training gilson com ...

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