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13

FC 203B FraCtion ColleCtor

 

|

 Quick RefeRence Guide

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Manual Mode

for manual collection, use the 

Start

,

 aDV

,

 Drain

 and 

enD Keys

. A delayed advance soft key advances the 

dispense head only after waiting until the effluent passes 

from the detector to the fraction collector. Refer to the 

user’s guide for more information about when to activate 

the delayed advance function.
Parameters:
delay time: _ . _ _ min
collect: _ _ _ . _ to _ _ _ . _ (collection windows)

Peak Modes

choose to collect peak and/or non-peak fractions. To 

detect peaks, the fraction collector must be connected 

to an analog output of a detector. The fraction collector 

can interpret the detector data to distinguish peaks from 

non-peaks. Refer to the user’s guide for instructions 

to connect the detector to the fraction collector 

multipurpose i/o port.

There is no peak-only mode. Peak or non-peak effluent 

must be subdivided either:
•  by time (from 0.01 to 99.99 min/tube) or
•  by drops (from 1 to 9999 drops/tube)

Summary of Contents for FC 203B

Page 1: ...Gilson Inc 3000 Parmenter Street PO Box 620027 Middleton WI 53562 USA Tel 608 836 1551 or 800 445 7661 F 608 831 4451 www gilson com FC 203B Fraction Collector Quick Reference Guide...

Page 2: ...2016 Gilson Inc All rights reserved LT1732 05...

Page 3: ...w 1 Customer Service 2 Notable Features 2 Collection Modes 2 Racks 2 Operation 3 Memory 3 HELP 3 Cycle Collection 3 Collection Windows 4 Key Controls 5 Hard Keys 5 Cycle Collection 11 Collection Modes...

Page 4: ......

Page 5: ...necessary plumbing and electrical connections Use the quick reference guide for information about control keys operating modes programming available racks input output control For more detailed infor...

Page 6: ...local Gilson representative Specific contact information can be found at www gilson com Notable Features Collection Modes Choose from several collection modes time drop peak plus time peak plus drop o...

Page 7: ...ols on page 5 for more information Memory The fraction collector remembers its last set of instructions e g the operating mode and the numerical values assigned to the various options even if the powe...

Page 8: ...es aiding in selective peak collection Remember four key points about collection windows It is always possible to collect from start to end designating the entire collection period as one collection w...

Page 9: ...of the unit Typically the dispense head will return to home after collecting into the last tube in the rack When using the multiple column option press END after the last fraction has been collected...

Page 10: ...r rather than tube number is displayed Typically tube advance is automatic but it is possible to manually initiate an advance by pressing ADV or advance remotely as described in the user s guide To un...

Page 11: ...el the next one or the first vessel in the next row 0 9 Press number keys to enter numerical values The decimal point where applicable does not have to be entered One easy way to enter a new number is...

Page 12: ...l effluent will be discarded Collection windows are optional Add up to ten windows Refer to the user s guide for detailed instructions Collect Press to collect the non peak effluent in peak modes Coll...

Page 13: ...account for the time it takes effluent to pass from the detector to the fraction collector Discard In peak modes press to divert the non peak effluent to the drain Drop Specifies collection in drop mo...

Page 14: ...ractionate peaks by time or by drop Refer to Collection Modes on page 2 for more information Quit Press to return to the previous display Rack Press to tell the fraction collector which rack will be u...

Page 15: ...per cycle Parameter in the Technical Menu press EDIT 2 allows for indicating how the fractions will be collected To collect each sample into the same set of tubes set the Tubes per cycle Parameter to...

Page 16: ...s _ _ _ _ min tube Collect _ _ _ _ to _ _ _ _ collection windows Optionally change min tube values during the run Enter new numbers and then press YES Drop Mode For collection of fractions by drop cou...

Page 17: ...ameters Delay time _ _ _ min Collect _ _ _ _ to _ _ _ _ collection windows Peak Modes Choose to collect peak and or non peak fractions To detect peaks the fraction collector must be connected to an an...

Page 18: ...collected the time it takes for the effluent to pass from the detector flow cell to the dispense head of the fraction collector This aids in the collection of purer peaks The user s guide explains how...

Page 19: ...s will not appear if discard non peak fractions was selected Peak width _ _ _ min Peak height _ _ _ mV or Peak level _ _ _ mV Delay time _ _ _ min Collect _ _ _ _ to _ _ _ _ collection windows It is p...

Page 20: ...et the rack code to 0 Code 0 rack For 80 12 mm diameter vials Code 1 rack For 80 13 mm diameter tubes Code 2 rack For 119 6 mm diameter tubes Code 4 rack For 80 12 mm diameter tubes Code 11 rack For 1...

Page 21: ...s guide for diagram Code 29 SE rack For 60 8 mm Eppendorf vials Set rack code to 29 if using vials without caps If using capped vials place vials in center two rows with caps facing outward set rack...

Page 22: ...d in peak modes Pin 1 is signal 10 100 mV Pin 2 is ground reference 3 4 Input A When activated by contact closure fraction collector will start a run or if already in run will advance to the next tube...

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