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Magnetic
Bead
Processing
Principle
Figure 2- 3 MPP
Magnetic
bead
processing
Magnetic bead processing can be divided into four separate steps:
⚫
Bind magnetic beads
⚫
Release magnetic beads
⚫
Rinse magnetic beads
⚫
Concentration
Bind
magnetic
beads
In the magnetic bead binding process, the magnetic rod is
completely inside the magnetic tip comb. The magnetic rod and
magnetic tip comb move slowly up and down in the microwell plate
to collect the magnetic beads to the top of the magnetic tip comb.
After the magnetic rod and magnetic tip comb are bound with the
magnetic beads, they are lifted from the microwell plate and
transferred to the next microwell plate.
Release
magnetic
beads
After the magnetic beads are collected, the magnetic rod and
magnetic tip comb are lifted from the microwell plate together and
transferred to the next microwell plate containing the reagent. The
magnetic rod and magnetic tip comb are placed together and the
magnetic rod is lifted.
The magnetic beads are released by moving the magnetic tip comb
up and down at high speed, and all the magnetic beads are fully
mixed with the substrate of the next reaction.