Genevac HT-4X Evaporation System
with XDS5 vacuum pump
User Manual
Original instructions
Part number 04-7415 Issue 1-16 August
2014
Page 1: ...Genevac HT 4X Evaporation System with XDS5 vacuum pump User Manual Original instructions Part number 04 7415 Issue 1 16 August 2014...
Page 2: ......
Page 3: ...ost and drain 30 Manual defrost and drain 31 Waste solvent container 31 Loading Samples 32 Sample holders 32 Use of correct accessories 32 Mass limit 33 Sample holders and swings 33 Loading the rotor...
Page 4: ...grammable software can store up to 100 methods or runs which can be configured independently or can be linked together to form multi stage programmes for a wide variety of solvent removal applications...
Page 5: ...ator screen and keypad Evaporation progress is shown by a graphical display with plots for temperature and pressure The evaporator features a built in condenser unit with intelligent feed back to prev...
Page 6: ...sing the evaporation system Only operate the system in a well ventilated space Make sure exhaust ventilation hoses are securely fitted and take solvent vapours away from the equipment and from the ope...
Page 7: ...om or from your local Genevac representative Electrical earthing Warning Risk of electric shock This equipment must be earthed The evaporation system is a safety class I product according to IEC class...
Page 8: ...enevac LyoSpeed fast lyophilisation process Condenser flusher For certain solvent applications periodic flushing of the condenser can help to maintain the performance of the evaporation system All vul...
Page 9: ...e holders are manufactured in solid aluminium and are anodised to improve heat absorption The contact area is maximised to provide good physical support optimum heat transfer and even heat distributio...
Page 10: ...nd the power is switched on Switch on the system using the evaporator power switch Note The pump power switch is a resettable circuit breaker it should be switched on and left permanently switched on...
Page 11: ...denser reaches 0 C if an attempt is made to start the run will commence once operational temperature has been reached Note For HCl systems an additional pump warm up sequence must complete before the...
Page 12: ...ure Stand by mode To enter stand by mode when the system is idle powered up but not evaporating press the STOP button The LCD screen continues to operate with reduced brightness all other systems powe...
Page 13: ...a graph of the last completed run use the cursor direction keys to show different segments in time 4 SETTINGS Press to display the Run Data screen for the selected run 5 Keypad Enter data for setting...
Page 14: ...N Currently selected method number 2 Status shows current evaporator status 3 Graphical data Shows progression of evaporation run with data for Channel 1 temperature Channel 2 temperature Pressure and...
Page 15: ...t on samples therefore these runs require the channel 2 temperature probe to be used For runs that use Heat flow or timed auto shut down the use of SampleGuard channel 2 is optional SampleGuard probes...
Page 16: ...n heat transfer across the plate For details of the range of heat transfer plates that are available contact your local Genevac representative to request a Genevac Accessories Brochure or visit the Ge...
Page 17: ...When the traces begin to converge this indicates the sample is approaching dryness Run parameters can be changed whilst the evaporator is operating refer to Advanced Operation Setting up a run Stoppi...
Page 18: ...eys indicated Skip The evaporator returns to the Run Select screen Drain The system opens the drain valve and pumps any waste solvent into the waste solvent container Defrost Enter the defrost menu Se...
Page 19: ...up a method The following section describes how to set up a new method the process is similar for overwriting an existing method Begin by selecting an empty slot from the SELECT RUN screen or select...
Page 20: ...changes Next Run Number Runs can be linked together enter the number of the run that requires to be linked and it will commence when the current run finishes Any number of runs can be linked together...
Page 21: ...xture Once selected solvents are highlighted in blue Select Done when the solvent or solvents have been selected For solvents that are not listed select Other and enter the solvent name manually no mo...
Page 22: ...amme the remaining run parameters Auto programmed run parameters are shown in blue but can be manually overwritten in which case they revert to being shown in white Alternatively select N No to contin...
Page 23: ...emperature SampleGuard Control Channel Channel 1 is the default setting The option to change to channel 2 is provided only to allow continued operation should a fault arise with channel 1 or its assoc...
Page 24: ...for the vacuum chamber to cool before evaporating volatile solvents or mixtures that may be prone to bumping this is unnecessary for most applications function Automatic Control To optimise the cryo...
Page 25: ...automatically if Dri Pure is selected see Pressure Control Regime Low Samples are subject to lower centrifugal force approximately 350 G This should be used for applications that do not require high r...
Page 26: ...in the sample holders at control temperature When sample dryness is detected the system automatically turns off the IR lamps and performs a final drying stage before stopping the run Sample Detection...
Page 27: ...Pure ramp Select this setting if bumping is anticipated Variable Dri Pure As Dri Pure but with added flexibility as settings for Dri Pure Start Pressure Dri Pure End Pressure Dri Pure Ramp Time and Fi...
Page 28: ...1 probe Channel 2 Temperature SampleGuard channel 2 probe Chamber Temperature temperature of vacuum chamber wall Condenser Gas Temperature temperature of the condenser coolant gas Pressure vacuum cham...
Page 29: ...End of Run Beeps On Off turns off repeating end of run alarm Options enable Auto Shutdown and Pre Run Checklist Maintenance for engineer use only Status displays information for fault diagnostics Fil...
Page 30: ...ing any of the other menu options START starts the highlighted run EDIT alternative route to the Run Data screen GRAPH shows the graph of the last completed run CLEAR clears the highlighted run profil...
Page 31: ...created on the data key before copying files to it To transfer data go to the OPTIONS menu and select FILE TRANSFER then select one of the following options COPY ALL METHODS FROM DATAKEY All methods...
Page 32: ...MSO are almost impossible to evaporate in one operation but using automatic defrost and drain methods can be set up to perform multiple operations without the need for manual intervention between stag...
Page 33: ...T and press the START button The defrost cycle will continue until the system senses that all solvent in the condenser pot is liquid or can be stopped manually by pressing the STOP button To drain liq...
Page 34: ...dance at all times Only use swings and sample holders that are approved for the system it is particularly important to check this if more than one Genevac system is in use Only use the tubes or vials...
Page 35: ...tions Do not exceed the mass limit under any circumstances In most cases with normal solvent volumes loaded Genevac sample holders will fall within this limit If in any doubt load up a swing and weigh...
Page 36: ...sample holder wells If using separate swings make sure the sample holders are located correctly in the swings If partially loading the rotor make sure sample holders swings are placed in a balanced co...
Page 37: ...st to redistribute the samples into four holders to help distribute the heat flow evenly between samples and to reduce mechanical stress on the rotor Alternatively two empty swings can be placed on th...
Page 38: ...SELECT RUN screen Runs 61 through to 98 are associated each run having a venting procedure linked to the end of the evaporation process which helps to remove residual vapours from the chamber Note Ru...
Page 39: ...te for fast lyophilisation methods it will also indicate when the monitored sample has frozen and when the lyophilisation process ends Use the data to optimise the method for example if it can be prov...
Page 40: ...ser to enter control parameters for pressure reduction cycles Select Crystallise and enter values for Pressure reduction mbar Pressure reduction time MM Atmosphere Vent Time MM The software feature is...
Page 41: ...one MEK Petroleum ether pet ether Butyl acetate 1 Propanol tert Butyl alcohol tert butanol 2 Propanal IPA iso propanol Chlorobenzene Propanone Acetone Chloroform CHCl3 Pyridine Cyclohexane Sodium hydr...
Page 42: ...sed to evaporate a mixture of DMSO and DCM Specific method settings would depend on variables such as volume mixture ratio and the type sample holder used 1 Select Variable Dri Pure and set a ramp fro...
Page 43: ...olume ml Your Volume Your Volume Your Volume Solvent MeOH H20 MeOH H20 MeOH H20 SampleGuard Control Temp C 40 40 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Chamber Tem...
Page 44: ...eOH H20 SampleGuard Control Temp C 40 0 0 40 SampleGuard Control Channel 1 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 50 Chamber Temperature control c preheat to 25 preheat to 0 preheat to 0 preheat...
Page 45: ...Volume Your Volume Your Volume Solvent DMSO or DMF DCM DMSO or DMF DCM DMSO or DMF DCM SampleGuard Control Temp C 40 40 40 SampleGuard Control Channel 1 1 1 CoolHeat Enable Pressure mbar 50 50 50 Cha...
Page 46: ...ain cycle manually Periodic maintenance Check the condition of the exhaust hose and waste solvent drain tubes Make sure the exhaust hose takes solvent vapours away from the system safely Avoid forming...
Page 47: ...e sample holder wells Spilled solvent can cause debris to stick to the sample holders causing high points that stress the glassware Clean off any adhered dirt or debris by washing the sample holders i...
Page 48: ...pressure of 16 mbar using a sample control temperature of 40 C Caution Purging with acetone is not recommended as vacuum seals within the evaporation chamber will be exposed Whilst every effort is mad...
Page 49: ...and a safe distance from any breakable objects or areas where entrapment could occur If required the evaporator can be bolted to the bench or trolley by its five mounting feet using M10 high tensile...
Page 50: ...hat an error occurs that that could affect the physical condition of the evaporator or its ability to safeguard the integrity of the samples a critical error the system will stop automatically A descr...
Page 51: ...cetonitrile or methanol Check the sample swings are able to move freely Clean the quartz glass using a suitable solvent such as methanol or acetone and a lint free cloth or paper towel Clean the swing...
Page 52: ...amples by reducing the pressure in the vacuum chamber by gradual increments Evaporation begins progressively and samples are contained due to the higher centrifugal force In the event of power failure...
Page 53: ...replaced by suitably trained personnel If a fuse blows repeatedly contact Genevac service for assistance Evaporator The evaporator fuse is located on the lower right hand side of the evaporator back p...
Page 54: ...opposing holders are balanced with 10 g IR lamp blown or cooling fan failed Contact Genevac Service No SampleGuard temperature display No SampleGuard signal Wait until rotor reaches full speed Note S...
Page 55: ...condensing Altitude Sea level to 1600 m Ventilation air gap 50 mm Ingress protection rating IP30 The evaporator is designed for use in a pollution degree 2 environment normally only no conductive poll...
Page 56: ...wing standards BS EN 61010 1 2010 Safety requirements for electrical equipment for measurement control and laboratory use General requirements BS EN 60204 1 2006 A1 2009 Safety of machinery Electrical...
Page 57: ...out as detailed in the Maintenance section of this User manual Power leads supplies with the system are for use with that system only If items are replaced by the owner only Genevac approved parts may...
Page 58: ...Aug 13 1 7 Display button description correction Connection diagram correction Sample holder image update Data transfer changed to Copying methods data 12 Nov 13 1 8 HCl option required for thionyl ch...
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Page 60: ...ng to press and may be subject to change without notice Some of the features and software functions described within this user manual may not apply to equipment manufactured before this manual s publi...
