GE SEMIPHOR TRANSPHOR Series, Operating Instructions Manual

Page: 43 / 69

Function Part
Optional: Mylar mask (opening approximately 2 mm smaller than the gels
on all sides) 9
Assemble parallel transfer stacks
To assemble a transfer stack with multiple gels, either place gels of the same thickness
side-by-side to create parallel stacks or place the gels in a vertical stack.
Follow the instructions below to assemble two parallel transfer stacks.
When transferring multiple gels, transfer efficiency depends on factors such
as gel thickness, gel position in the stack, transfer buffer, membrane type, and,
most importantly, the characteristics of the protein. The gel closest to the anode
generally transfers better than the gel that is further away from the anode. It
is preferable to lay gels side-by-side rather than stacking them.
Tip:
Action Step
Follow the instructions in Preparations, on page 38 . 1
Follow the instructions in Assemble a transfer stack with a single gel, on
page 39 , while referring to Illustration of a transfer stack with multiple gels,
on page 40 .
2
NOTICE
Stack each layer with care, with edges parallel. As each
layer is added, remove all air pockets by rolling a clean
pipet from the center to the edges. Add a few drops of
buffer to trouble areas to help remove air pockets.
Note:
For best results, the two transfer stacks should be centered on the electrode
panels.
Repeat step 2, to create a second transfer stack next to the first. Make sure
that there is no electrical contact between the two stacks. 3
Place the cover on top of the transfer stack.
Note:
Do not remove the cover until after the transfer is complete in order to prevent
stack components from moving.
4
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA 43
5 Operation
5.1 Assemble transfer stack