Function
Part
Optional: Mylar™ mask (opening approximately 2 mm smaller than the gel
on all sides)
5
Preparations
Follow the instructions below to prepare for making a transfer stack.
Action
Step
Rinse the anode and cathode with distilled water.
1
Cut away the wells and/or stacking gel section from the gel.
2
Equilibrate the gel in transfer buffer if required.
3
If the membrane is larger than the gel, place a Mylar mask at the bottom of
the transfer stack. The Mylar mask prevents current to bypass the gel. Follow
the steps below.
4
1
Measure the gel.
2
Cut an opening in the center of a Mylar mask. Make sure that the size
of the opening is roughly 2 mm smaller than the gel.
WARNING
Be careful when cutting the masks. The blades are
sharp and may cause cuts.
3
Place the mask in the base of the unit. Make sure that the opening of
the mask is in the center of the base.
Calculate the number of blotting paper layers. Divide the volume of buffer
required for the run by the volume of buffer a layer of blotting paper can
absorb. Up to 300 mL buffer is required for larger gels and for transfers of
60 minutes.
5
Volume of buffer that will be ab-
sorbed
Blotting paper size
50 mL
21 × 26 cm
20 mL
14 × 16 cm
38
SEMIPHOR TRANSPHOR UNIT series Operating Instructions 29281777 AA
5 Operation
5.1 Assemble transfer stack
