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GE SE 250 Mighty Small II, Operating Instructions Manual

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GE SE 250 Mighty Small II Operating Instructions Manual Download Page 34

5.2

Assembly

Place the gel sandwich in the SE
250/260 Mighty Small II

WARNING

Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.

Action

Step

Rinse away the gel overlay with distilled water and drain any excess water.

1

If installing a self-cast or precast 10 × 8 cm gel sandwich, orient the sandwich
so that the notched plate faces the gasket, notches at the top. Set the bottom
of the sandwich on the supporting ledges in the bottom of the lower chamber
and center the plate so that the gasket seals both sides.

2

Place the gel sandwich in the SE
250/260 Mighty Small II

WARNING

Acrylamide is a neurotoxin. Always wear gloves and observe all
laboratory safety procedures.

34

SE 250/260 Mighty Small II Operating Instructions 29281629 AA

5 Operation

5.2 Assembly

Summary of Contents for SE 250 Mighty Small II

Page 1: ...SE 250 260 Mighty Small II Operating Instructions Original instructions ...

Page 2: ...ecautions 20 2 2 Labels 22 2 3 Emergency procedures 23 2 4 Recycling information 24 2 5 Declaration of Hazardous Substances DoHS 26 3 System description 28 4 Installation 29 5 Operation 31 5 1 Preparations 34 5 2 Assembly 40 5 3 Electrophoresis run 42 6 Maintenance 44 7 Troubleshooting 47 8 Reference information 48 8 1 Specifications 49 8 2 Ordering information 50 8 3 Health and Safety Declaration...

Page 3: ...ons of safety notices regula tory information and intended use of the SE 250 260 Mighty Small II In this chapter See page Section 4 1 1 About this manual 5 1 2 Important user information 7 1 3 Regulatory information SE 250 260 Mighty Small II Operating Instructions 29281629 AA 3 1 Introduction ...

Page 4: ...ns covers the SE 250 260 Mighty Small II The illustration below shows the SE 250 260 Mighty Small II Illustrations The images and annotations in this document are for illustrative purposes only The configuration of individual products may vary and therefore illustrations may not reflect the actual system delivered 4 SE 250 260 Mighty Small II Operating Instructions 29281629 AA 1 Introduction 1 1 A...

Page 5: ...ccommodates one or two 10 8 cm gel sandwiches The SE 250 260 Mighty Small II accommodates one or two 10 8 cm or 10 10 5 cm gel sandwiches In both SE 250 260 Mighty Small II units the upper buffer chamber is formed when the notched side of a gel sandwich is sealed against the silicone rubber gasket The upper buffer chamber core serves as a heat exchanger if cooling is required The core is hollow an...

Page 6: ...situation which if not avoided could result in minor or moderate injury It is important not to pro ceed until all stated conditions are met and clearly understood NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment Notes and tips A note is used to indicate information that is important for trouble free and optimal use of the product Note A t...

Page 7: ...ng information Information Requirement GE Healthcare Bio Sciences AB Name and address of manufacturer Björkgatan 30 SE 751 84 Uppsala Sweden In this section See page Section 8 1 3 1 EU directives 9 1 3 2 Eurasian Customs Union 10 1 3 3 Regulations for USA and Canada 11 1 3 4 Other regulations and standards SE 250 260 Mighty Small II Operating Instructions 29281629 AA 7 1 Introduction 1 3 Regulator...

Page 8: ...ity is available on request Title Directive Low Voltage Directive LVD 2014 35 EU Restriction of Hazardous Substances RoHS Directive 2011 65 EU CE marking The CE marking and the corresponding EU Declaration of Conformity is valid for the in strument when it is used according to the Operating Instructions or user manuals and used in the same state as it was delivered from GE except for alterations d...

Page 9: ...on Requirement See Manufacturing information Name and address of manufacturer Telephone 46 771 400 600 Telephone number of manufacturer GE Healthcare LLC Importer and or company for obtain ing information about importer GE Healthcare Life Sciences Presnenskaya nab 10C 12th floor RU 123 317 Moscow Russian Federation Telephone 1 7 495 411 9714 Fax nr 7 495 739 6932 Email LSrus ge com SE 250 260 Migh...

Page 10: ...Canada NRTL certification This symbol indicates that the product has been certified by Intertek which is a US Oc cupational Safety and Health Administration Nationally Recognized Testing Laboratory NRTL 10 SE 250 260 Mighty Small II Operating Instructions 29281629 AA 1 Introduction 1 3 Regulatory information 1 3 3 Regulations for USA and Canada ...

Page 11: ...ic Products China RoHS Standards applied to this product Standard requirements fulfilled by this product are summarized in the table below Description Standard Safety requirements for electrical equipment for mea surement control and laboratory use Part 1 General requirements IEC EN 61010 1 UL 61010 1 CAN CSA C22 2 No 61010 1 Technicaldocumentationfortheassessmentofelectrical and electronic produc...

Page 12: ...ortant WARNING Before installing operating or maintaining the product all users must read and understand the entire contents of this chapter to become aware of the hazards involved In this chapter See page Section 13 2 1 Safety precautions 20 2 2 Labels 22 2 3 Emergency procedures 23 2 4 Recycling information 24 2 5 Declaration of Hazardous Substances DoHS 12 SE 250 260 Mighty Small II Operating I...

Page 13: ...ategories General precautions Personal protection Using flammable liquids Installing and moving the product Power supply System operation Maintenance Always follow the instructions below to avoid injury when using the SE 250 260 Mighty Small II General precautions WARNING Before installing operating or maintaining the product all users must read and understand the entire contents of this chapter t...

Page 14: ...r replacements WARNING The safety lid must be in place before connecting the power leads to a power supply WARNING Any liquid on the equipment must be dried off before connecting the power supply CAUTION Circulate only water or 50 50 water ethylene glycol through the heat exchanger Never use anti freeze or any organic solvent in the heat exchanger CAUTION Never introduce anti freeze or any organic...

Page 15: ...tions for safe operation and maintenance of this product WARNING Spread of biological agents The operator must take all necessary actions to avoid spreading hazardous biological agents The facility must comply with the national code of practice for biosafety CAUTION Handlethe glass componentswith care Wear appropriatepersonal protective equipment PPE Using flammable liquids WARNING A fume hood or ...

Page 16: ...e connecting cables before moving the equipment CAUTION The electrophoresis unit is heavy especially when filled with buffer Handle the unit with care to avoid personal injury Power supply WARNING Power cord Only use power cords with approved plugs delivered or approved by GE WARNING The safety lid must be in place before connecting the power leads to a power supply 16 SE 250 260 Mighty Small II O...

Page 17: ...ays wear gloves and observe all laboratory safety procedures WARNING Never exceed the operating limits stated in this document and on the system label Operation of the product outside these limits can damage equipment and cause personal injury or death WARNING Turn all power supply controls off and disconnect the power leads before removing the safety lid WARNING Always disconnect the high voltage...

Page 18: ...imiting the voltage and current to specified ratings NOTICE After initial monitoring do not leave the unit unattended for more than 45 min before checking the progress of the bands and the buffer level NOTICE If running only one gel block off the unused part of the core with a glass plate Do not fill this side with buffer Maintenance WARNING Decontaminate before maintenance To avoid personnel bein...

Page 19: ...any maintenance task WARNING Decommissioning Decontaminate the equipment before decom missioning to make sure that hazardous residues are removed NOTICE Cleaning Keep the exterior of the instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the instrument dry completely before use SE 250 260 Mighty Small II Operating Instructions 29281629 AA 19 ...

Page 20: ...se contact an authorized repre sentative of the manufacturer for information concerning the de commissioning of equipment This symbol indicates that the product does not contain toxic or hazardous materials in excess of the limits established by the Chi nese standard GB T 26572 Requirements of concentration limits for certain hazardous substances in electrical and electronic products and can be re...

Page 21: ... Safety labels The table below describes the various symbols that may be found on the product Description Symbol text Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation Warning High Voltage Always make sure that the system is disconnected from electric power before re moving the lid SE 250 260 Mig...

Page 22: ...shutdown In an emergency situation shut down the power supply in accordance with its emergency procedure Power failure In case of power failure to the SE 250 260 Mighty Small II the run is interrupted immedi ately Restart after emergency shutdown or power failure To restart the run after an emergency shutdown or power failure follow these steps Action Step Make sure all connections are in place 1 ...

Page 23: ...of service the different materials must be separated and recycled according to national and local environmental regulations Disposal of electrical components Waste electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioningof th...

Page 24: ...on of Pollution Control Label 该标志表明本产品不含有超过中国标准GB T26572 电子信息产品中有毒有害物 质的限量要求 中限量的有毒有害物质 报废后可以进行回收处理 不能随意丢弃 This symbol indicates that this electrical and electronic product does not contain any hazardous substances above the maximum concentration value established by the Chinese standard GB T 26572 and can be recycled after being discarded and should not be casually discarded 24 SE 250 260 Mighty ...

Page 25: ...部件的某一均质材料中的含量超出 GB T 26572规定的限量要 求 此表所列数据为发布时所能获得的最佳信息 0 Indicates that this hazardous substance contained in all of the homogeneous materials for this part is below the limit requirement in GB T 26572 X Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB T 26572 Data listed in the table represents b...

Page 26: ...of the SE 250 260 Mighty Small II Illustration of the instrument The illustration below shows the SE 250 260 Mighty Small II instrument 1 2 3 4 5 6 8 7 Function Part Color coded leads 2 1 26 SE 250 260 Mighty Small II Operating Instructions 29281629 AA 3 System description ...

Page 27: ...hamber 8 The following parts are included but not shown in the illustration Glass plates Notched alumina plates Gel seal 1 4 oz Spacer Mate Well locating decal Power supply with a minimum rating of 250 V 50 mA constant current or constant voltage is required but not included Note SE 250 260 Mighty Small II Operating Instructions 29281629 AA 27 3 System description ...

Page 28: ...ake sure that the system is placed on a stable level bench with adequate space for ventilation Unpacking procedure Unwrap all packages carefully Inspect all visible parts for damage or missing pieces If any damage is observed record this on the receiving documents and inform your GE representative Make sure to keep all packing material for damage claims or to use should it become necessary to retu...

Page 29: ...e electrophoresis unit WARNING Acrylamide is a neurotoxin Always wear gloves and observe all laboratory safety procedures WARNING Never exceed the operating limits stated in this document and on the system label Operation of the product outside these limits can damage equipment and cause personal injury or death CAUTION Do not operate with buffer temperatures above the maximum specifiedtechnicalsp...

Page 30: ...t to specified ratings NOTICE After initial monitoring do not leave the unit unattended for more than 45 min before checking the progress of the bands and the buffer level NOTICE If running only one gel block off the unused part of the core with a glass plate Do not fill this side with buffer In this chapter See page Section 31 5 1 Preparations 34 5 2 Assembly 40 5 3 Electrophoresis run 30 SE 250 ...

Page 31: ...horesis unit Load liquid samples after the gel sandwich is installed Maintenance before use Rinse the instrument before each use Before using the first time disassemble the unit completely and wash with a dilute solution of a laboratory detergent and thoroughly rinse with water and distilled water Check the gasket Periodically remove the gray silicone rubber gasket from the core In spect for nicks...

Page 32: ...other to the outlet 3 Secure the connections with the hose clamps 4 Install the upper buffer chamber core CAUTION Circulate only water or 50 50 water ethylene glycol through the heat exchanger Never use anti freeze or any organic solvent in the heat exchanger Action Step Steady the lower chamber with one hand and then hold the core with the other hand 1 Position the lower chamber on the positionin...

Page 33: ...o remove the core depress both release tabs and lift 4 1 2 3 Description Part Handle 1 Release tabs 2 2 Coolant port 2 3 SE 250 260 Mighty Small II Operating Instructions 29281629 AA 33 5 Operation 5 1 Preparations ...

Page 34: ...0 8 cm gel sandwich orient the sandwich so that the notched plate faces the gasket notches at the top Set the bottom of the sandwich on the supporting ledges in the bottom of the lower chamber and center the plate so that the gasket seals both sides 2 Place the gel sandwich in the SE 250 260 Mighty Small II WARNING Acrylamide is a neurotoxin Always wear gloves and observe all laboratory safety pro...

Page 35: ...ith the bottom of the upper buffer chamber core 2 If installing a self cast or precast 10 10 5 cm gel sandwich orient the sandwich so that the notched plate faces the gasket notches at the top Set the bottom of the sandwich on the supporting ledges in the bottom of the lower chamber and center the plate so that the gasket seals both sides see figure below The 10 10 5 cm gel sandwich fits against t...

Page 36: ...dwich or if running only one gel clamp a plain glass plate on the unused side of the core to prevent a possible short circuit with the unused electrode Do not fill this chamber with buffer if no gel sandwich is in place 4 Sample preparation and loading If applicable cast the stacking gel in the unit Calculate the stacking gel monomer solution volume measure the distance in cm from the top of the r...

Page 37: ...e solution near the spacer at the side of the sandwich and allow it to flow across the surface unaided 5 Allow a minimum of one hour for the gel to polymerize 6 To prepare the sample follow these steps Action Step Increase liquid sample density with 10 glycerol or sucrose 1 Add a tracking dye 2 For SDS protein gels use 2 treatment buffer to denature both liquid and dry samples in a test tube 3 To ...

Page 38: ...holds approximately 75 mL 2 Load the sample into the wells using a fine tipped microsyringe The width of the wells depends on the number of wells per comb For the volume re quired to fill to 1mm well depth see the table below 3 Volume of sample µL per 1 mm depth Comb thickness mm No of wells 1 5 1 0 0 75 19 1 12 7 9 5 5 5 8 9 7 2 4 8 3 6 10 4 4 2 9 2 2 15 2 9 18 Final assembly CAUTION Devices must...

Page 39: ... lower gel buffer contact surface is exposed the colored plastic tape must be removed Place the safety lid on the unit 2 Plug the color coded leads into the jacks of an approved power supply The red lead plugs into the red output jack and the black lead plugs into the black output jack 3 Optional cooling Begin circulating cold water or a chilled 50 50 water ethy lene glycol solution 4 SE 250 260 M...

Page 40: ...ng dye reaches the bottom of the gel Watch the buffer level in the upper buffer chamber and if necessary replenish it before it falls below the level of the notched plate A small volume of buffer may leak past a chipped plate or nicked gasket or it may wick out through the gel NOTICE After initial monitoring do not leave the unit unattended for more than 45 min before checking the progress of the ...

Page 41: ...r buffer chamber core 4 Gently loosen and then slide away both spacers 5 Slip an extra spacer or a Wonder Wedge into the bottom edge to prevent breaking the ears of the notched plates and separate the plates The gel usually adheres to the alumina plate 6 Carefully lift the gel from the plate and lay it into a tray of stain or fixative 7 SE 250 260 Mighty Small II Operating Instructions 29281629 AA...

Page 42: ...Always disconnect power from the instrument before performing any maintenance task General procedures Immediately after each use rinse the unit with water and then rinse thoroughly with distilled water Do not use organic solvents abrasives strong cleaning solutions or strong acids or bases to clean the chambers Handle the upper buffer chamber core with care to prevent damage to the banana plugs Al...

Page 43: ...k Please complete the checklist in the On Site Service Health and Safety Declaration Form or the Health and Safety Declaration Form for Product Return or Servicing depending on whether the instrument is going to be serviced on site or returned for service respectively Copy the form you need from Section 8 3 Health and Safety Declaration Forms on page50 SE 250 260 Mighty Small II Operating Instruct...

Page 44: ...t exchanger Never use anti freeze or any organic solvent in the heat exchanger CAUTION Never introduce anti freeze or any organic solvent into any part of the instrument Organic solvents will cause irreparable damage to the instrument Potential problems Corrective action Error description To reduce the running temperature Smile effect on the buffer front Circulate coolant through the upper buffer ...

Page 45: ...tion and polymerization Bands are skewed or distorted De gas the stacking gel solution and avoid trapping air bub bles under the comb teeth Overlaythe runninggel withwater saturatedn butanolbefore polymerization begins to avoid forming an uneven gel surface Check sample preparation Dialyze or desalt the sample Centrifuge or filter sample before loading to remove particu lates Near the buffer front...

Page 46: ...tion Heat samples for no more than 1 2 minutes at 100 C Store on ice after heating Store sample on ice before it is denatured Add protease inhibitors if necessary to prevent proteolytic degradation of sample Store samples to be frozen in aliquots to prevent repeated freezing and thawing Store at 40 to 80 Pour a taller stacking gel For best results allow a stacking gel height of 2 5 times the heigh...

Page 47: ...l II The chapter also includes ordering information and the Health and Safety Declaration form for service In this chapter See page Section 48 8 1 Specifications 49 8 2 Ordering information 50 8 3 Health and Safety Declaration Forms SE 250 260 Mighty Small II Operating Instructions 29281629 AA 47 8 Reference information ...

Page 48: ...mum current 45 C Maximumgeltemperature Indoor use 4 C to 40 C Environmental operating conditions Humidity up to 80 Altitude up to 2000 m II Installation category 2 Pollution degree 16 5 16 16 cm Dimensions W H D 6 5 6 3 6 3 in 2 7 kg Weight 48 SE 250 260 Mighty Small II Operating Instructions 29281629 AA 8 Reference information 8 1 Specifications ...

Page 49: ... information For product codes and information about how to order please see www gelifesciences com SE 250 260 Mighty Small II Operating Instructions 29281629 AA 49 8 Reference information 8 2 Ordering information ...

Page 50: ...othersuitablesurvey Adequate space andclearance is providedto allowsafe access forinstrumentservice repairor installation Insome cases this mayrequirecustomerto move equipmentfrom normaloperatinglocation prior to GE arrival Consumables suchas columns orgels have beenremovedor isolatedfromthe instrument andfrom any areathat may impede access tothe instrument All buffer waste vessels are labeled Exc...

Page 51: ...ardous biological substances pleasespecify OtherHazardous Chemicals please specify Equipment must be decontaminatedpriortoservice return Pleaseprovide atelephone number whereGE cancontact youfor additionalinformationconcerningthe system equipment Telephone No Liquid and orgas in equipment is Water Ethanol None empty Argon Helium Nitrogen Liquid Nitrogen Other please specify Equipment type ProductN...

Page 52: ... P Prerequisites 5 Product return or servicing 51 Purpose of this manual 4 R Recycling information decontamination 23 disposalofelectricalcompo nents 23 Regulatory information 7 10 S Safety information 12 Safety label Description 21 Safety notices 6 Safety precautions Installation 16 introduction 13 Maintenance 18 Power supply 16 System operation 17 Samples loading 36 preparation 36 SE 250 260 Mig...

Page 53: ...E Healthcare Bio Sciences AB Björkgatan 30 751 84 Uppsala Sweden GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences Corp 100 Results Way Marlborough MA 01752 USA HyClone Laboratories Inc 925 W 1800 S Logan UT 84321 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan For local office contact information v...

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