Appendix 4. Non-radioactive
labeling and detection systems
Labelling and detection
Sensitivity Time from
Duration of
Strip before Quantification Recommended application
system
hybridization light output re-probing
to detection
AlkPhos Direct (RPN3690)
0.06 pg
1 hour
5 days
yes
no
Single copy Southern and
Northerns
ECL Direct (RPN3000)
0.5 pg
1 hour
1–2 hours
no
no
High target applications
eg. colony/plaques
RPN2510EUM Appendix 4 Rev C 06/2007
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Summary of Contents for RPN2512E
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