14
Electrophoresis Power Supply EPS 3501 XL User Manual 18-1130-22 Edition AD
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DEUT
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ESP
AÑOL
IT
ALIANO
Choosing step or gradient programming
Press SET ENTER to confirm step ( ) or use
/
keys to choose gradient ( ⁄ ). Confirm
with SET ENTER.
Note that your choice of gradient or step programming applies for all phases within the
program and you will only be asked to choose one of them when programming the first
phase.
Choosing step means that voltage, current and power limiting values are programmed.
The electrophoresis will be controlled by one of these limiting values, which means that
it is run at either constant voltage, current or power. The EPS 3501 XL automatically
switches over the controlling parameter according to programmed limits and
conductivity variations in the system. Thus the controlling parameter can switch within a
phase.
Table 1 and Fig 4 illustrate a step program. The programming and running of this
application are shown as Figs 3 and 7.
Fig 4.
Programming the voltage limiting profile in STEP mode. The parameters shown are the same
as those listed in Table 1.
Table 1.
The parameters of a step program.
Phase
Voltage
Current
Power
Volthours
number
(V)
(mA)
(W)
(Vh)
1
1000
10
10
1000
2
500
10
5
120
3
2000
20
15
1000
4
3500
30
25
1500
Choosing gradient ( ), means that a
voltage endpoint
for the actual phase is
programmed together with
current and power limiting values.
A linear voltage gradient
is made with zero (for the first phase) or the programmed endpoint of the phase before
(for the next phases) as starting point and the programmed endpoint as endpoint. The
electrophoresis will be controlled by this voltage gradient provided the limiting current or
power is not attained. The EPS 3501 XL thus automatically switches over the controlling
parameter according to the programmed limits and conductivity variations in the
system.
3500
2000
1000
500
1000 Vh
Phase 1
Phase
2
120 Vh
1000 Vh
1500 Vh
Phase 3
Phase 4
1
Time (h)
Voltage
(V)
2
5 Operation
