Funke Gerber CryoStar I Manual Download Page 12

Common

: Mode can be changed to Celsius, Osmol, Hortvet or alcohol mode. The

base value for added water in Celsius mode, the user and the id can be changed.

Temperature

: The temperature of the cooling bath and the beat temperature can be

changed.

Calibration

: The used solutions for A- and B-calibration can be changed. And with

Pre-Calibration

 a Pre-calibration can be started.

Plateau

: Plateau Search, Fixed time and Maximum are available. The parameters

can be changed depending on the selected plateau mode, see 12.1

Stirrer

: The stirring and beat amplitude and the stirring frequency can be changed.

To test the different settings, two different fields are available. By  

Stirrer On

  the

agitator starts moving. The agitator should be set to approx. 2 mm movement width
by   changing  

stir   amplitude  

.   If   the   agitator   is   considered   from   the   side,   the

oscillation distance can be seen well. The agitator movement should be checked
regularly   and   readjusted   if   needed.  

The   agitator   frequency   should   not   be

changed.   It   has   already   been   adjusted   at   the   factory.

  In   the   case   that   the

frequency has to be adjusted, it should be set to 4 Hz higher than the resonance
frequency. The resonance frequency is determined, e.g. by starting at 150 Hz and
constantly increasing the frequency afterwards. The resonance frequency is at the
point where the agitator movement achieves a maximum. By 

beat- amplitude 

the

amplitude   when   beating   can   be   changed   and   with  

beat

  the   amplitude   can   be

checked. For this purpose, hold a sample vial filled with 2.5 ml of water on the
measuring head so that agitator and thermistor dip in the water and activate the
agitator by touching 

beat

. On the one hand, the agitator should strongly strike the

glass   wall   of   the   sample   vial   so   that   the   freezing   process   is   well   and   quickly
triggered:   on   the   other   hand   it   should   not   be   too   strong   in   order   to   prevent
damaging the sample vial.

12

Summary of Contents for CryoStar I

Page 1: ...Manual CryoStar I Article Nr 7150 January 2016...

Page 2: ...n 15 6 1 Preparing for calibration 15 6 2 Perform calibration 16 a Adjust the machine to the calibration standard 16 b A calibration 16 c B calibration 16 6 3 Pre calibration 16 7 Maintenance 17 7 1 R...

Page 3: ...rantee conditions 23 10 1 Guarantee period 23 10 2 Guarantee terms 23 11 Specification 23 12 Appendix 24 12 1 Principle of the freezing point determination 24 a Fixed time 25 b Plateau search 25 c Max...

Page 4: ...gases Always adhere to hazard warnings and safety tips when using reagents chemicals and cleaning agents b Measures to the working safety The device should not be opened as live parts might be expose...

Page 5: ...ls 1 stand for 27 sample vials 1 Phillips screwdriver 1 Allen key 1 copy of these Operating Instructions Please check the delivery is complete 3 3 Installation location Select an even level place in y...

Page 6: ...ll up the cooling system with coolingbathliquid Please make sure that there is enough cooling bath liquid in system by pressing the pump button in Settings System pump The liquid should be flowing vis...

Page 7: ...is setup as an serial device 2 USB A port for USB mass storage USB Sticks formatted preferably with FAT file system can be connected Usage of USB hard disks is not recommended 3 Ethernet port 4 serial...

Page 8: ...bration solutions is shown 4 Stop stops a running measurement or calibration 5 show the actual setup user and probe number By touching both fields can be changed 6 temperature changes from red to blue...

Page 9: ...messages and possible solutions are available in 9 The status area on the right shows the state of several components They all should be green A red state means a serious technical problem has occurr...

Page 10: ...iptions of error codes see 9 For the last 100 measurements the charts are also available These can be shown by selecting a measurement in the history A pop up window will show the chart and the possib...

Page 11: ...e can be changed by the user On the left is the navigation area on the right the parameters Recommended settings Designation Settings A calibration value 0 000 C or 0 408 C B calibration value 0 557 C...

Page 12: ...llation distance can be seen well The agitator movement should be checked regularly and readjusted if needed The agitator frequency should not be changed It has already been adjusted at the factory In...

Page 13: ...be installed technical all technical parameters that user normally do not need to change If parameters need to be changed please contact the service At least the error log and information to used sof...

Page 14: ...The measurement can be stopped by touching Stop After reaching the triggering temperature beat temperature e g 2 00 C the machine changes over to plateau display This means the temperature range is di...

Page 15: ...using two point calibration an A calibration and a B calibration Funke Gerber provides two different A calibration standards 0 000 C and 0 408 C and two different B standards 0 557 C and 0 600 C As th...

Page 16: ...Measurement with calibration liquid B Place sample vial with calibration liquid B in the cooling bath opening Start measurement see 5 This is only a preliminary measurement for flushing purposes c B c...

Page 17: ...wards Rotating the thermistor slightly makes its removal easier Now you can start installing the new thermistor The new thermistor is inserted into the bore provided with a slight rotary movement Impo...

Page 18: ...indicates that this product must not be placed in your household waste It is your responsibility to take any harmful waste from electrical and electronic equipment to designated collection points By...

Page 19: ...o the switches or motor b uncalibrated or defective thermistor The instrument tests the current thermistor value when starting a measurement or calibration Its electrical resistance is known to be a f...

Page 20: ...ion for this is a rise in temperature of at least 0 1 C This is always the case with water or calibration solutions if the stirring rod is set in such a way that it beats hard against the glass wall T...

Page 21: ...because the values of the new thermistor after calculation with the calibration constants of the old thermistor result in wrong temperatures Therefore a so called pre calibration is necessary where th...

Page 22: ...s the thermistor must be replaced d Agitator error a The agitator is not oscillating freely The agitator must be able to move freely in the slot provided for it It must never touch the thermistor at a...

Page 23: ...on Measurement range 0 0000 C bis 1 5000 C Resolution 0 0001 C 0 1 m C Repeatability 0 0020 C 2 0 m C Calibration 2 point Sample volume 2 0 ml bis 2 5 ml Sample rate up to 40 h Connectors 1 x USB A 1x...

Page 24: ...mple is completely frozen solid and the temperature then drops The graph on the left shows the principal temperature curve However if the curve in the area of the plateau is considered with higher res...

Page 25: ...template with a window being placed over the cooling down curve The window width 20 to 23 seconds is usual and the window height 0 2 m C to 1 m C is usual are specified and the plateau is considered...

Page 26: ...achines Directive in its design and construction and in the configuration put on the market by us This declaration is invalid if the machine is modified without our agreement Machine description CryoS...

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