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USER MANUAL

Streamer

System

STR-4000

05-12-17

Rev 6.0

Culturing Cells in a Mechanically Active Environment

™

Flexcell International Corporation



2730 Tucker Street, Suite 200



Burlington, NC 27215

800-728-3714



(919) 732-1591



FAX: (919) 732-5196



www.flexcellint.com



INTERNATIONAL CORPORATION

Summary of Contents for Streamer System STR-4000

Page 1: ...0 Culturing Cells in a Mechanically Active Environment Flexcell International Corporation 2730 Tucker Street Suite 200 Burlington NC 27215 800 728 3714 919 732 1591 FAX 919 732 5196 www flexcellint co...

Page 2: ...onfiguration 8 Pull down Menus 9 Operate Menu 10 Manual Mode 10 View Data 11 Configure Users 12 Configure Regimes Setup Parameters 13 Configure Testing Apparatus 15 How to enter the proper values for...

Page 3: ...ed to apply fluid induced shear stress to cells grown in a monolayer The system includes a six chamber laminar flow device designed to hold 75 x 25 x 1 mm Culture Slips Fig 1 Cells are cultured on the...

Page 4: ...ct on the medium bottle that is connected to the short bent tubing in the bottle using the other 2 0 6 m long piece of silicone tubing 6 Before the first use run deionized water through the entire sys...

Page 5: ...green light is beside the number 17 you will want the HP light to be off Once this is set the display on the pump will read the flow rate for that particular tubing as the pump is running Consult the...

Page 6: ...s thereby creating a bubble trap for any air bubbles that may accidentally enter the system Do the same with the second pulse dampener Once this process is complete allow flow to continue and go to th...

Page 7: ...ctional video Streamer Assembly on Flexcell s website http www flexcellint com videos instruct htm for a demonstration of how to assemble the Flexcell Streamer and associated tubing to run with a Mast...

Page 8: ...roceeding with any experiments MAIN PANEL GENERAL INFORMATION TAB Function The default main panel allows the user to verify that the system is running and to stop the tests at any time Buttons and Fie...

Page 9: ...ndow will appear Start Start the experiment Stop Terminate the experiment This button is only active when an experiment is running Pause Suspend the experiment The pump will stop but the test regimen...

Page 10: ...List of regimes created by the previously selected user Select from list by using the mouse to highlight the desired regime Apparatus List of configured flow devices Select the device that will be us...

Page 11: ...l Q Operate Manual Mode Manually control the pump without setting up an experimental regimen View Data View shear stress data from a previous experiment Users Add and remove user names Configure Regim...

Page 12: ...shear stress value is not shown on this panel since it will depend on the tubing size and flow chamber used Instructions 1 Enter the flow set point pump speed either by entering a number in the box or...

Page 13: ...The complete file path to the data file being viewed Table Contents of the experimental data log file Export File Export data to a spreadsheet compatible format Print Print a copy of this panel to the...

Page 14: ...e system Delete User Delete users from the system Help Online help not currently available Return Exit this panel and return to the Main panel Instructions To add a user 1 Type the name into the User...

Page 15: ...ter updates of the pump parameters default is 1 second Time Between Data Log to File Time interval between each computer sampling of the experimental flow data Default value is 10 seconds For an extre...

Page 16: ...which step to go back to The GoTo step must always be a step number before the current step Loop Indicates how many times to loop between the GoTo step and the current step Summary Table This table i...

Page 17: ...endix of the manual for your device Buttons and Fields Testing Apparatus List of all flow devices available Name When a testing apparatus is selected this field and the parameters will be updated Flow...

Page 18: ...the Main panel Any changes that have not been saved will be discarded HOW TO ENTER THE PROPER VALUES FOR YOUR DEVICE Please check the Appendix of the manual for your device for the proper b and h valu...

Page 19: ...a saving and the presence of valves in the flow system Buttons and Fields Pump Serial Port Com 1 is the default port This should be changed only if there is a conflict with this port on your computer...

Page 20: ...FT V4 2 NOTES When running a regime in StreamSoft V4 2 do not run other applications on the same computer The communication timing to the pump and Osci Flow requires full CPU availability If another p...

Page 21: ...ld 5 Click on Save Regime The regime name should appear in the Regimes for Selected Users field at the top 6 Specify the Flow Type FWD REV PULSED OSCILLATION ON HI OFF LO times only when using the pul...

Page 22: ...direction of the flow is going from the vertex of the angle to the open end of the angle Leave the pulse dampener in this position until the fluid comes through the outlet fitting again then lay the...

Page 23: ...m components back to the incubator Put the Phar Med tubing back into the MasterFlex pump head and clamp the head down 17 Unscrew the clamp on the Phar Med tubing to open the flow path to full capacity...

Page 24: ...e growth surface at 10 25 000 cells cm2 in 3 to 5 ml of medium Be sure to plate cells on the side where the Teflon border is printed Once the cells are attached additional medium is added and the cult...

Page 25: ...6 System Flow Rate ml min Shear Stress dyn cm 2 0 0 0 37 1 0 74 2 0 111 3 0 148 4 0 185 5 0 222 6 0 259 7 0 296 8 0 332 9 0 369 10 0 406 11 0 443 12 0 480 13 0 517 14 0 554 15 0 591 16 0 628 17 0 665...

Page 26: ...distributor This warranty is extended only to the original purchaser customer unless a transfer of ownership is approved by Flexcell in writing e LIMITED LIABILITY Flexcell or its distributor s only...

Page 27: ...Ave NW Suite 306 Washington DC 20015 Phone 5506 4646 Fax 5505 7433 Web www sellex com Japan LMS CO LTD 3 6 7 Hongo Bunkyo ku Tokyo 113 0033 Japan Phone 81 3 5842 4171 Fax 81 3 5842 4180 Email intldpt...

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