F
LEXCELL
®
I
NTERNATIONAL
C
ORPORATION
1
INTRODUCTION
BioFlex
®
Cell Seeders (Fig. 1) confine cells during plating to the central area of a BioFlex
®
membrane. The central region of the membrane is positioned directly over the 25 mm diameter
cylindrical loading posts in the Loading Station
used during application of equibiaxial substrate
strain. Cells plated in this manner are subjected to defined strains when using the equibiaxial posts
in the Loading Stations (Fig. 2). The BioFlex
®
Cell Seeder
™
is inserted into the BioFlex
®
Baseplate
(Fig. 3), and the 6-well BioFlex
®
plate plus the gasket are placed on top of the Cell Seeder. Vacuum
draws the membrane into the defined central region of the membrane during cell plating and
adhesion. Once cells have adhered to the membrane, vacuum is released, and cells can be cultured
in the usual manner.
Figure 1.
6-well BioFlex
®
Cell Seeder Station in
a BioFlex
®
baseplate well.
Figure 2.
The results of using a BioFlex
®
Cell
Seeder when plating cells to confine them to the area
directly above the 25 mm cylindrical loading posts
(purple = cells stained with Crystal Violet stain).
Figure 3.
BioFlex
®
Cell Seeders in a BioFlex
®
Baseplate.
