9
Fein
Microscope Operation
Using the 100x Immersion Oil Objective:
Start with the 4x objective and focus on
the sample. Place a drop of immersion oil
(1) on the specimen. Rotate the nosepiece
counterclockwise so the 100x objective is in the
light path and the oil seals the space between
the objective and the cover slip. Use the fine
focus knob to obtain a clear image. Make sure
there are no air bubbles in the oil. If there are,
rotate the nosepiece slightly to remove them.
After use, wipe the lens with a tissue moistened
with microscope cleaning solution.
Using Filters:
Filters can increase the contrast in microscopy
images. The filter sits on top of the light as
shown at the right. The RB20 accepts a 45mm
diameter filter.
C-Mount assembly:
Loosen the trinocular head set screw (1)
and remove the trinocular port dust cap
(2). Remove any dust cover caps from the
c-mount (3). Insert the c-mount into the
trinocular port as image shows at right and
tighten the set screw (1). Connect the camera
to the c-mount threads (4) and re-tighten the
set screw. When the eyepieces are in focus,
adjust the focus on the c-mount to parfocal
the camera with the eyepieces.
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