Mi50 Inverted Microscope Operation Instructions
13
Fein
Polarization uses a polarizer and
analzyer. Remove the dust cover (1)
and insert the polarizer (2) as shown.
The polarizer will click into place.
Remove the color filter when using
the polarizer. Remove the dust cover
(3) from the analyzer socket and insert
the 360
°
rotating analyzer (4) face up
as shown. The polarizer and analyzer
are orthogonal when the 360
°
rotating
analyzer is zero adjusted. Rotating the
dial (6) on the analyzer can change the
orthogonal state of the polarized light.
Loosen the set screw (1) on the
trinocular port and remove the dust
cap (2). Remove the dust covers from
the c-mount and screw the camera
onto the c-mount threads (3). Insert the
c-mount on the trinocular port and re-
tighten the set screw. Look through the
eyepieces to ensure a clear image, then
engage the beam slitter (5) to direct
light to the camera. If the image is not
clear, adjust the c-mount focusing (4)
until the image is clear.
In brightfield, adjust the focusing using
a 10x or 20x objective to make sure the
image is clear. Push the polarizer and
analyzer into the light path to ensure
both are orthogonal - or that the field
of view is darkest. Loosen the locking
screw (1) on the nosepiece, remove the
dust cap and insert the DIC slider (2)
fully and re-tighten the locking screw.
Rotate the DIC fine adjustment knob (3)
to unify interference colors in the field
of view. Adjust the aperture diaphragm
at the same time for best contrast.
