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Fein

IRB20 Inverted Microscope User’s Manual

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Summary of Contents for IRB20

Page 1: ...Fein IRB20 Inverted Microscope User s Manual info feinoptic com ...

Page 2: ...Microscope Components Eyepieces Condenser Lamp Housing Nosepiece Mechanical Stage Rheostat On Off Power Siedentopf Trinocular Head C Mount Adapter Phase Slider Beam Splitter Coarse Focus Fine Focus Stage X Y Control ...

Page 3: ...ilter Slider 6 Lamp Housing 7 Condenser 8 Round Insert on Stage 9 Fixed Stage 10 Carrying Handle 11 Objective Lens 12 Nosepiece 13 Power Socket 14 Fuse Box 15 Power Switch 16 Surplus Water Plate 17 Mechanical Stage X Y Knob 18 Light Rheostat Control 19 Coarse Fine Focus Control 20 Attachable Mechanical Stage 21 Beam Splitter Knob ...

Page 4: ... stage Move the 10x objective lens into the light path Focus on the sample using the coarse focus and then the fine focus Adjust the brightness using the rheostat control Adjust the interpupillary distance and the eyepiece diopter Adjust the aperture diaphragm Once the sample is in focus filters may be placed in the optical path ...

Page 5: ...as shown in the image at right Set the power switch to off O before replacing a bulb or fuse and wait until the lamp is cool The power switch is located on the side of the microscope Microscope uses 5W LED bulb Part M40 001 Voltage range of 100 240V 47 63Hz is supported Turn the rheostat control knob 1 to the minimum and turn the power switch 2 to the on I position Rotate the rheostat knob to incr...

Page 6: ...e focus tension ring clockwise to create more tension and keep the stage from drifting Do NOT rotate the coarse fine focus knobs in opposite directions at the same time Adjust the eyetubes to set the interpupillary distance for your eyes If multiple people use the microscope daily note the number of your interpupillary setting for ease of future setup Set both eyepieces to 0 scale on the diopter L...

Page 7: ...the set screw and pull the eyepiece out of the eyetube FIXED STAGE Insert the round plate 1 into the fixed stage Install the stage clips 2 into the slot on the fixed stage MECHANICAL STAGE Insert the round plate 1 into the stage Install attached mechanical stage 2 with screws 3 provided Place petri dish or stage inserts 4 into stage as needed ...

Page 8: ...ope prior to changing the bulb 1 Remove the screw with the allen wrench 2 Lift the illumination cover 3 The bulb is located at the front of the housing Once the cover is removed unscrew the two screws in the image shown below that lie on either side of the wires leading to the bulb ...

Page 9: ... wires connected to the bulb Unscrew the 2 screws holding the bulb to the housing Screw the new bulb into the housing Re solder the wires back onto the bulb in the same positions they were in prior to bulb replacement Replacement Bulb M40 001 5W LED FUSE REPLACEMENT Turn off and unplug the microscope prior to fuse replacement The fuse box is installed below the power socket Fuse 5x20mm 2A 250V ...

Page 10: ...nd put the phase slider in the position that matches the phase objective magnification Remove the eyepiece and insert the phase centering telescope Use the phase centering screws to align the bright and dark rings as shown in the illustrations below Image at left shows what the rings might look like prior to alignment Image at right shows once they are aligned ...

Page 11: ...tion Sample is not placed in correct place on stage Adjust sample and holder Phase contrast observation is poor Annular spot did not focus with dish holder Readjust phase alignment Culture dish bottom is not flat Use standard culture dish Coarse focus knob is too tight Tension is too tight Loosen tension adjustment ring Nosepiece slides down automatically Tension is too loose Tighten tension adjus...

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