15
Column Operation
This section provides general guidance on operating the Evolve® range of columns. It includes a
hydrostatic leak test to establish that the column is installed correctly and advice for the
preparation of the chromatography media.
Further advice specific to each media can be obtained from the media manufacturers; this section
only provides a general guidance.
Hydrostatic Leak Test
The purpose of this test is to check that there are no leaks from the column and the column is
ready to use. Due to the materials of fabrication, when the column is initially exposed to pressure
the column will flex. It is necessary to perform the hydrostatic leak test using a two step
procedure.
1.
Ensure fixed end is correctly located and fixings tightened to the correct torque
setting. See table below.
Column Diameter
mm
Torque Setting
Nm
Torque Setting
Ft lbf
70mm
20
14.75
100mm
20
14.75
140mm
20
14.75
2.
Fill the column with WFI or high quality water and position the adjuster at the
operating bed height.
3.
Ensure the adjuster seal is wetted with buffer or other suitable liquid prior to
assembly. This helps ‘lubricate’ the seal to ensure correct seating.
4.
Activate the adjuster seal
5.
Ensure all air is removed from the column and any connections.
6.
Attach a calibrated pressure gauge to the top inlet of the column
7.
Raise the column pressure until 3bar (45 psi) and close bottom inlet valve. Wait
15mins for the pressure to stabilize.
8.
Open the valve and increase the pressure to maximum working pressure 4bar (58psi).
9.
Wait 15mins for the pressure to stabilize. Check the pressure and ensure pressure is 4
bar (58psi).
10.
Wait for 15mins.
Due the nature of materials it is likely there will be a small pressure drop however; this
should not exceed 0.1 bar.
Chapter
4
Summary of Contents for 100
Page 1: ...70mm 100mm and 140mm Chromatography Columns ...
Page 44: ......
