13
6)
Open
the
gas
flow
out
of
the
regulator
by
turning
the
“T”
bar
counterclockwise.
This
begins
the
flow
of
gas
to
the
controller.
Set
the
regulator
flow
rate
at
50
CFH
for
the
TT
‐
8100
or
33
CFH
for
the
TT
‐
4000.
7)
The
system
has
two
preset
timers
for
species
selection.
Press
up
for
“ADULTS”
or
down
for
“NEONATES”
on
the
front
of
side
control
box.
The
red
“RUN”
light
indicates
the
euthanasia
cycle
is
operating.
8)
During
the
first
initial
run,
adjust
the
regulator
flow
rate
to
50
CFPH.
This
adjustment
will
assure
a
humane
process.
Animals
will
first
be
anesthetized
with
minimal
stress
and
then
euthanized
while
asleep.
Exceeding
this
flow
rate
may
introduce
stress
to
animals
and
result
in
operation
outside
of
AVMA
guidelines.
(This
regulator
adjustment
is
typically
only
required
the
first
time
using
the
system
and
when
a
full
CO
2
tank
is
first
put
on
line.)
9)
The
system
will
cycle
through
two
stages:
Stage
1
Charging:
Gas
flows
through
the
chamber
for
8:30
minutes
,
fully
charging
the
chamber
with
CO
2
.
Stage
2
Dwell:
Gas
flow
stops
and
the
chamber
remains
fully
charged
with
CO
2
for
5
minutes
.
Stage
3
Exhaust:
Blower
will
activate
for
3.5
minutes,
chamber
will
unlock
and
return
to
ready
state
(green).
Start
Toggle
Adult
Neonates
