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User and Service 
Manual 

Thank you for purchasing this Esco Biological Safety Cabinet. 

Please read this manual thoroughly to familiarize yourself with the 

many unique features and exciting innovations we have built into 

your new equipment. Esco provides many other resources at our 

website, www.escoglobal.com, to complement this manual and 

help you enjoy many years of productive and safe use of your Esco 

products. 

 

For Technical Service, contact 
North America 

Esco Technologies, Inc. 
2940 Turnpike Drive, Units 15-16 •Hatboro, PA 19040, USA 
Toll-Free USA and Canada 1-877-479-3726

 

Tel 215-441-9661  •  Fax 215-441-9660 
us.escoglobal.com  •  [email protected] 

 

Rest of the World 

Esco Micro Pte. Ltd.  
21 Changi South Street 1  •  Singapore 486777 
Tel +65 6542 0833  •  Fax +65 6542 6920 
www.escoglobal.com  •  [email protected] 

Class II  
Biological Safety Cabinet

 

Esco Airstream Class (AC2 - E/S/D/G/N) II BSC User and Service Manual 
Version F - Released July 2013

 

Summary of Contents for Class II

Page 1: ...of productive and safe use of your Esco products For Technical Service contact North America Esco Technologies Inc 2940 Turnpike Drive Units 15 16 Hatboro PA 19040 USA Toll Free USA and Canada 1 877...

Page 2: ...change Sentinel and Airstream are registered trademarks of Esco Material in this manual is provided for informational purposes only The contents and the product described in this manual including any...

Page 3: ...3 1 2 Operating Motorized Sash Window Only for BSC with motorized sash window 12 3 1 3 Using Sash Window 12 3 2 Starting and Shutting Down the BSC 12 3 2 1 Turning on the BSC 12 3 2 2 Turning off the...

Page 4: ...hapter 7 Troubleshooting 344 7 1 Electrical and Mechanical Troubleshooting 51 7 2 Software Troubleshooting 533 Chapter 8 Engineering Details 533 8 1 AC2 _E_ Engineering Drawing 54 8 2 AC2 _S_ Engineer...

Page 5: ...ons Failure to perform preventive maintenance Using accessories parts or components not supplied by Esco Damage by fire floods or acts of God Customer modifications to the product Consumables such as...

Page 6: ...duct Appendix Warranty Listings Biological Safety Cabinets Laminar Flow Cabinets HEPA Filtered Cabinets except Streamline brand 4 years limited Laboratory Fume Hoods 2 years limited Ductless Fume Hood...

Page 7: ...ns given in this manual may result in damage to the unit injury to operating personnel and or poor equipment performance Any internal adjustment modification or maintenance to this equipment must be u...

Page 8: ...EC It is marked with the following symbol Esco sells products through distributors throughout Europe Contact your local Esco distributor for recycling disposal Directive 2002 95 EC on Restriction on...

Page 9: ...following directives 2006 95 EEC The Low Voltage Directive and its amending directives 2004 108 CE The Electromagnetic Compatibility Directive and its amending directives has been designed to comply w...

Page 10: ...ectives 2004 108 CE The Electromagnetic Compatibility Directive and its amending directives has been designed to comply with the requirement of the following Harmonized Standard Low Voltage EN 61010 1...

Page 11: ...k tray Figure 1 1 AC2 Gen 3 general parts 8 Exhaust Collar Optional 9 Exhaust ULPA Filter 10 Electrical Panel 11 Curved Front Panel 12 Display Panel 13 Fluorescent Lamps 14 Side Window 15 IV Bar Retro...

Page 12: ...oves the menu options upwards and downwards o Increases and decreases corresponding value inside one of the menu options o Moves the sash window upward and downward for motorized sash BSC o For access...

Page 13: ...ety Cabinets o To go back to the previous level of the menu options o To access maintenance mode from error condition 2 2 Menu Options Please refer to the following diagram for complete reference to a...

Page 14: ...ng upon activation of the unit This is to ensure that the sensors the blower and the control system are stabilized as well as to ensure the work zone is purged of contaminants The default setting is 3...

Page 15: ...e BSC has three modes and two of which normal mode and quickstart mode can be used in daily activity Both of these modes can be seen and accessed when you enter the FAN PIN The last mode maintenance m...

Page 16: ...00 DISABLED FAN PIN restricts access to fan control and some parts of the menu settings and set mode User must enter the four digit PIN before switching the fan on or off This feature prevents unautho...

Page 17: ...sent This option is used to reset the UV lamp hour meter The UV lamp hour meter indicates how long the UV lamp has been in operation Maximum counter is set at 2 000 hours 100 The counter value can be...

Page 18: ...y position Pressing UP button again while the stopwatch function is activated will stop and resume the timer Use the DOWN button to exit the stopwatch function and reset the timer The timer in the sto...

Page 19: ...nge UV LIFE Shows percentage of UV lamp life based on UV Lamp Hour Meter UV TIMER Shows the UV timer value default is 60 minutes Maximum value is 00 minutes infinite on MUTE TIMER Shows the mute timer...

Page 20: ...s typically used during the night to maintain basic level of containment where the cabinet is not used by the operator The mode can be accessed by pressing the FAN button when the cabinet is in operat...

Page 21: ...e lights will switch off automatically Lower Sash from Safe Height Position When the sash is at safe operating height pressing the down button and holding it will cause the sash to move down to the fu...

Page 22: ...turn on when this height is reached Note When Quickstart mode is selected fan will turn on as well without pressing the fan button 2 Turn on the fan by pressing the FAN button Input the Fan PIN if as...

Page 23: ...urface in the BSC Place aerosol generating instruments as far back in the BSC as possible and at least 150 mm 6 inches from clean items materials Place air turbulence generating equipment such as cent...

Page 24: ...ed before and after working with susceptible organisms However it should not be the sole decontamination agent Chemical decontamination agent should still be used There should be minimal amount of mat...

Page 25: ...tly be carried out by means of formaldehyde fumigation or using other decontamination agents such as chlorine dioxide or hydrogen peroxide Decontamination process should only be carried out by qualifi...

Page 26: ...16...

Page 27: ...window with appropriate disinfectant and glass cleaner afterward Use a damp cloth to clean the exterior surface of the BSC particularly on the front and top in order to remove dust that has accumulate...

Page 28: ...ons to airflow Care must be taken as the area is contaminated Figure 4 1 Paper catch access Pre filter Installation 1 Insert the pre filter to the opening of the paper catch as shown in image 1 above...

Page 29: ...2 Unscrew the two aluminum covers using Philip head screw driver 3 Unscrew the two aluminum switch brackets using the Philip head screw driver 4 Tilt up the sash glass freely for cleaning 4 2 Mainten...

Page 30: ...annot be determined o Re certification indicates the displayed airflow deviates by 0 02 m s 4 fpm from actual measured velocities only for products with airflow velocity displays Decontamination befor...

Page 31: ...nsure the fan and lamps are turned on The cabinet should then be put to maintenance mode In Maintenance Mode all alarms are defeated and the window can be moved without the light being switched off 1...

Page 32: ...cate speed controller positioned toward right side of electrical panel 4 Plug the multimeter probes to the Motor Voltage Sampling Port 5 Adjust the airflow by adjusting the speed controller 5 3 2 1 In...

Page 33: ...unless and until the inflow and or downflow velocity can no longer be maintained within the specifications 1 Before the filters are removed from the BSC their contaminated faces should be taped off us...

Page 34: ...d AC2 _S_ 1 Move the Sash Window down until it reaches the armrest Note For motorized sash BSC remove the window straps after moving the sash window to the lowest position 2 Remove red plenum cover us...

Page 35: ...te For motorized sash BSC remove the window straps after moving the sash window to the lowest position 3 Remove red plenum cover using Phillips screwdriver 4 Remove the secondary exhaust filter mounti...

Page 36: ...rom downflow filter Caution Be careful when adjusting the bolt adjusters This might damage the filter gasket 4 Take out the downflow filter carefully 5 To install new filter just do a reverse procedur...

Page 37: ...fter the front panel is secured 3 The fluorescent lamp s is located behind the blue panel Disconnect the old one s and replace with the new one s 5 4 4 UV Lamp Replacement Procedure 1 Carry out necess...

Page 38: ...located on top of the cabinet The sensor can easily be accessed in these cabinets 1 Start by removing the damper unscrew on top of the cabinet for AC2 _E_ AC2 _S_ damper not applicable for AC2 _D_ and...

Page 39: ...hnut sensor housing from the bracket Figure 5 10 Remove doughnut sensor housing 6 Install the new sensor it comes with the doughnut sensor housing into the bracket and connect the sensor cable connect...

Page 40: ...decontamination formalin gas presents the following health risks External contact can cause irritation to skin eyes and mucous membranes Inhalation in small concentrations can cause coughing nausea an...

Page 41: ...n Being a true gas it spreads quickly without the need of pulsing the BSC s blower It can rapidly kill the micro organisms with high efficacy with just 1 hour contact time User must note that the conc...

Page 42: ...1 One port located in front opening or side wall penetrating the work zone area 2 One port located on top of the exhaust filter The generator used defines the port function as described below Steris B...

Page 43: ...To seal the cable first make an shape aluminum tape around the cable and make a 5 cm flat portion going to both directions Then paste the aluminum tape to the floor 6 Put the bottom part of the decont...

Page 44: ...n be used for troubleshooting but cannot be used for motor voltage measurement Phillips screwdriver Insulated jumper cables The Electrical Panel and Component Layout Open the front panel the electrica...

Page 45: ...d correctly to the wall outlet NOTE Some cabinets have 2 cordsets Disconnect the power cord at the cabinet and measure the AC voltage between the live and the neutral terminal of the cord If the volta...

Page 46: ...l blocks the problem is between connector A and the terminal strips If voltage is not present at connector A but voltage is measured at the female connector for the line cord at the top of the cabinet...

Page 47: ...ard and the display and key pad The larger flat cable is for the Display The smaller flat cable is for the LEDs keypad membrane If the LCD Display is on but not the LEDs in keypad membrane check conne...

Page 48: ...a new LCD to the LCD port on main board If the new LCD functions properly this indicates the old one is defective If the new LCD does not work replace the large Flat Ribbon Cable If the LCD still doe...

Page 49: ...button on control panel Enter the Fan PIN number if required default is 0001 The LED for FAN should illuminate and the FAN should start If the FAN does not operate Proceed to the next step If the LED...

Page 50: ...k the voltage between the BLUE Neutral terminal block and the end terminal on the LS1 circuit J2 on relay board Detection of voltage at the end terminal with no cable indicates that the F1 fuse is goo...

Page 51: ...try to turn the potentio to maximum and minimum to check for response If ECMS responds to the adjustment check PWM output with the multimeter If ECMS produces PWM output ECMS is likely not defective...

Page 52: ...next step Low building supply voltage if new cabinet check this first Refer to the electrical layout and find motor voltage Test Point shown in Figures 4 2 and 4 3 NOTE The voltage adjustments should...

Page 53: ...w is not good please adjust motor speed controller accordingly until alarm is turned off and the LCD shows nominal airflow reading After this if LCD still shows AIRFAIL Proceed to the next step The se...

Page 54: ...ered please refer to next possible cause part A 2 Just after calibration ADC IFA value should be close to ADC IFN value If not please adjust the speed controller to make both values closer by at least...

Page 55: ...the next step Problem 6 Excessive Blower Noise Cause Corrective Action Resonance Note Requires a Certifier or qualified personnel Locate the motor speed controller Measure the motor voltage and recor...

Page 56: ...elay board Locate LS7 circuit and measure AC voltage between BLUE NEUTRAL terminal block and the end terminal with Red cable on the three terminal strip J13 on relay board If there is voltage to the e...

Page 57: ...Sash not in UV state Lower the sash to UV mode position If SASH FULLY CLOSED is not displayed on the LCD please refer to the Magnetic Switch troubleshooting section Switch on the UV lamp by pressing t...

Page 58: ...he K2 coil to Connector D and the neutral wiring from K2 to the DC Power supply If wiring connections are OK visually inspect K2 relay for any burnt or flash marks If burnt or flash marks are present...

Page 59: ...ED is on If there is power to the relay and it is energized Check voltage across Connector B pin 1 to 2 and pin 4 to 5 If there is power through Connector B pins remove socket outlets from wall Check...

Page 60: ...that the correct DC line voltage is connected properly and turned on Replace the ECMS with a new one Figure 11 2 ECMS showing the power indicator The output voltage cannot be adjusted by turning the...

Page 61: ...c Switch 3 Magnetic Switch 4 Description Close Open Open Open Sash is fully opened ALARM is given ALARM can be muted Open Close Open Open Cabinet sash is at nominal height ready to use Open Open Close...

Page 62: ...if air fails No No SASH ALARM Sash Position Detection Yes Yes Yes Current Time Display Yes Yes Yes Sash Alarm Yes not mutable Yes not mutable Not Applicable Fan Control Yes without WARM UP PURGING ti...

Page 63: ...53 Class II Biological Safety Cabinets Chapter 8 Engineering Details 8 1 AC2 _E_ Engineering Drawing...

Page 64: ...54 8 2 AC2 _S_ Engineering Drawing...

Page 65: ...Required Exhaust With Optional Thimble Exhaust Collar 260 cmh 153 cfm 320 cmh 189 cfm 538 cmh 317 cfm 615 cmh 362 cfm 823 cmh 485 cfm Static Pressure For Optional Thimble Exhaust Collar 28 Pa 0 11 in...

Page 66: ...56 8 4 AC2 _D_ Engineering Drawing...

Page 67: ...57 Class II Biological Safety Cabinets...

Page 68: ...58 8 5 AC2 _G_ Engineering Drawing...

Page 69: ...ficiency 99 999 at 0 1 to 0 3 micron as per IEST RP CC001 USA 99 999 at MPPS as per EN 1822 H14 EU Sound Emission NSF ANSI 49 62 3 dBA 65 5 dBA EN 12469 61 3 dBA 62 5 dBA Fluorescent Lamp Intensity lu...

Page 70: ...60 8 7 AC2 2N7 Engineering Drawing A C 2 2 N 7 E n g i n e e r i n g D r a w i...

Page 71: ...61 Class II Biological Safety Cabinets...

Page 72: ...l Thimble Exhaust Collar 260 m3 h 153 cfm Static Pressure For Optional Thimble Exhaust Collar 28 Pa 0 11 in H2O ULPA Filter Efficiency Downflow 99 999 at 0 1 to 0 3 micron as per IEST RP CC001 USA 99...

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