CultureCoin
®
User Manual Rev. 7.0
13
8.
Check for air bubbles in the culture wells after the equilibration. Remove any
bubbles with a stripper tip.
9.
Load the embryos in the wells. Make sure the embryos are located very precisely
in the center of the culturing well and not on the side slope.
10.
Optionally fill the pH reservoir and insert the silicone plug.
11.
Incubate the CultureCoin
®
in the MIRI
®
TL.
10 CultureCoin
®
placement in the compartment
Before starting the time-lapse, it is essential to place the CultureCoin
®
in the
compartment properly. To ensure the correct position of the CultureCoin
®
(so that the
camera can identify all the wells), place the CultureCoin
®
in its place and secure its
position by pushing it
downwards and towards you
, then to the
left edge
.
Figure 10.1
Positioning CultureCoin
®
in the compartment
11 Removing the CultureCoin
®
lid
CultureCoin
®
lid does not have a lot of free movement on the dish while it is closed, but
the lid may move slightly during transportation. The lid can rotate a bit on the
CultureCoin
®
and get stuck on the dish edges. As a result, it may be harder to open it.
In such a case, there is a specific technique for the CultureCoin
®
lid opening.
Hold the dish
and
lift the lid from the cut edge
.