CultureCoin
®
User Manual Rev. 7.0
12
The correct way to assemble the CultureCoin
®
dish using both lids:
Figure 8.3
Assembled CultureCoin
®
dish
The smaller lid is put on the CultureCoin
®
dish facing the 4 handles up. If the
lid is put upside down, it will be loose and not fit correctly.
The lid (2) is used to protect liquid content until the CultureCoin
®
dish is placed in MIRI
®
TL multiroom IVF incubator. Before putting CultureCoin
®
into MIRI
®
TL multiroom IVF
incubator, this lid should be removed.
The small lid (3) can optionally be used to maintain stable conditions if the CultureCoin
®
dish is removed from the MIRI
®
TL
family’s
multiroom IVF incubator several times during
the incubation cycle.
The small lid (3) is not used if the CultureCoin
®
dish is not removed from the MIRI
®
TL
famil
y’s
multiroom IVF incubator during the entire incubation cycle.
9 Use
1.
Unpack both the lid and dish in an aseptic environment.
2.
Leave the dishes with the lids on for off-gassing in the aseptic environment for 12
hours.
3.
Warm the dishes on a heated stage or an incubator before filling them with media.
4.
Fill all the wells with the app. 25 µl of precultured equilibrated culture media in
an aseptic environment. Filling of the washing wells is optional. They can contain
approximately 23 µl each.
5.
Check under a microscope for any visible air bubbles and remove them with a
stripper tip.
6.
Fill the oil area with a confluent layer of suitable mineral or paraffin oil to cover
the culture media. Do not overfill.
7.
Put the lid on the dish and leave it for 4 hours of equilibration in the CO
2
incubator.