H
OSPI
-G
ARD
®
I
SO
C
LEAN
®
and
I
SO
C
LEAN
®
UV
Operation Manual
Page 15
4.15. Unit Decontamination
NOTE:
Use proper protocol, gowning and protective measures during unit
decontamination.
Should decontamination of the unit be required, the following is an adaptation by
Annex G. of the Recommended Microbiological Decontamination Procedure from NSF
49, May 1992. Confirm with appropriate Biosafety and Industrial Safety Professionals
that the procedures meet your facility’s guidelines.
4.15.1.
Paraformaldehyde Decontamination
4.15.1.1. Relocate the unit as described in Section 4.14 to a controlled
access, non-public area with a non-porous floor, good ventilation
and a dedicated exhaust directly outside the building. A new
prefilter is not required. Attach and seal a flexible hose to the
dedicated exhaust. Place the other end of the exhaust hose near
the unit.
4.15.1.2. Calculate the total volume of the unit by multiplying the height, width
and depth. The total IsoClean
volume is 13 cubic feet (61 in. x 24
in. x 15.5 in.).
4.15.1.3. Multiply the total volume of the unit by 0.3g/ft
3
to determine the gram
weight of Paraformaldehyde required. Decontamination of the
IsoClean
requires 3.9 grams (12 ft
3
x 0.3g/ ft
3
) of
Paraformaldehyde.
4.15.1.4. Remove the intake grille and wipe it down with an appropriate
surface decontaminant.
4.15.1.5. Place an unplugged heating device, such as a commercially
available electric frying pan with the thermostat set at 232.2º to
246.1º C (450º to 475º F) inside the unit through the removed
prefilter access panel. Spread the Paraformaldehyde evenly over
the heating surface of the electric frying pan.
CAUTION: The auto-ignition temperature of Paraformaldehyde is 300
o
C
(572
o
F). Place a hot plate, beaker of water, and temperature
and humidity indicators in the unit next to the pan that will
contain the Paraformaldehyde.
4.15.1.6. Enclose all sides of the unit with heavy gauge plastic film and tape
in place while leaving the film that will close the space formerly
occupied by the intake grille free. The power cord should be coiled
and taped to the unit and sealed under the plastic film. Seal the film
to the floor on which the unit stands.
4.15.1.7. Determine the temperature and humidity inside the unit.