Operating Manual
qTOWER³ / qTOWER³ GReal-Time PCR Thermal Cycler
Page 1: ...Operating Manual qTOWER qTOWER G Real Time PCR Thermal Cycler...
Page 2: ...30 37079 G ttingen Germany Phone 49 551 50 68 60 Fax 49 551 50 68 666 Email service analytik jena com General Information http www analytik jena com Documentation Number 10 3107 002 23 Edition B 05 2...
Page 3: ...pair 11 3 6 Behavior during emergencies 11 4 Design and function 12 4 1 Design connections and control elements 12 4 2 Function 14 4 2 1 Fluorescence spectrometer 14 4 2 2 PCR thermocycler 16 4 2 3 He...
Page 4: ...f contents qTOWER qTOWER G 4 9 1 3 Moving the device in the laboratory 33 9 2 Storage 34 10 Disposal 35 11 Specifications 36 11 1 Technical data 36 11 2 Ambient conditions 38 11 3 Standards and direct...
Page 5: ...ntions Instructions for actions occurring in chronological order are numbered and combined into action units Warnings are indicated by a warning triangle and a signal word The type source and conseque...
Page 6: ...CR products Analytik Jena offers a series of color or FRET modules with up to six being able to be si multaneously installed in the device The replacement and retrofit of color or FRET modules is easy...
Page 7: ...qTOWER qTOWER G Intended use 7 LOGAN Julie EDWARDS Kristin SAUNDERS Nick ed Real Time PCR Current Tech nology and Application Norfolk UK Caister Academic Press 2009...
Page 8: ...ains controlled substances Ana lytik Jena GmbH warrants that these substances will not be released from the device within the next 25 years provided the device is employed as intended General warning...
Page 9: ...ervice or trained specialist per sonnel authorized by Analytik Jena Observe the guide values and adhere to the legally mandated limits for lifting and carry ing without auxiliary means For safety reas...
Page 10: ...ns of the electrical components switch off the device at the main switch immediately and disconnect the mains plug from the grid Before opening the device must be isolated from all circuits Work on th...
Page 11: ...maintenance and repair Maintenance of the device is always carried out by the Analytik Jena customer service department or specialist personnel trained and authorized by Analytik Jena Unautho rized m...
Page 12: ...1 Front view 1 Upper part with fluorescence spectrom eter 2 Lock with handle 3 LED for status display 4 Lower part with thermocycler The device is opened by folding back the upper part and the cover...
Page 13: ...switch 7 Type plate 8 Interface for the connection cable to the connected PC The voltage selector switch is located on the bottom of the device behind a cover It can be used to adjust the operating v...
Page 14: ...ted lid Color modules Stepper motor Spindle motor Spindle Lenses in the measuring head Figure 6 Schematic representation of the fluorescence spectrometer Light source A long life sturdy four color LED...
Page 15: ...green arrows 1 2 3 4 5 6 7 8 9 10 Figure 7 Schematic representation of the light beam path through a filter color module 1 Aspherical lens 2 Excitation filter 3 Collimator lens 4 Light source 5 Aspher...
Page 16: ...of the reaction tubes above the block surface level Furthermore the heated lid guarantees regardless of the con sumables used a reliable contact between the reaction tubes and the thermal block durin...
Page 17: ...ss 2 Protection class of the housing 3 Safety symbol Attention Observe the accompanying documentation 4 Device number 5 Year of manufacture 6 Disposal instructions Do not dispose of as domestic waste...
Page 18: ...dust and caustic fumes Keep the ventilation slits free and do not obstruct them with other devices 5 1 1 Spatial requirements NOTICE Type and source of the hazard When opening the device the upper de...
Page 19: ...rating voltage and correcting it if it does not match the voltage supplied at the site Connecting the device to the mains and the PC Installing software on the PC Remove the device the connection cabl...
Page 20: ...e sup ply voltage Switch on the PC Install the software on the PC Observe the information in the soft ware manual for this Switch on the device via the power switch When first switched on the device i...
Page 21: ...is switched on Carry out manual device identification if this has not already occurred To do this se lect menu item Extras Device identification in the software Status LED The status LED is located on...
Page 22: ...g contact Sample blocks in 96 well SBS format are suitable for use with 0 2 mL individual tubes 8 well strips and 96 well micro titer plates These and other consumables can be ordered from Analytik Je...
Page 23: ...uitable corre sponding optical lids Prepare a real time PCR project with complete information on the PCR run floures cence measurement and sample layout of the PCR plate Figure 12Position A1 on the sa...
Page 24: ...ing errors check these options for elimination Error code Cause Remedy Device cannot be switched on Check the power supply Replace the fuses Replace the power cable Sample cups are damaged during meas...
Page 25: ...ase contact Analytik Jena customer service immediately 8 1 Cleaning the housing WARNING Risk of short circuit Switch off the device before all maintenance and cleaning work and remove the power plug f...
Page 26: ...les carefully Wipe spilled samples or reagents immediately with an absorbent cloth or piece of paper If the device is used for the analysis of infectious material great care must be taken as the devic...
Page 27: ...om the compartment Remove the old fuses and replace them with identical types Reinsert the fuse holder into the compartment and close the lid NOTICE The fuse compartment cannot be opened if a power ca...
Page 28: ...the inside of the lid Close the lid Remove the four outer screws on the rear of the device on the upper part the hood is fastened with Pull the hood forward slightly Pull the hood forward for enough...
Page 29: ...l it rests flat on the filter wheel Screw on the color module with the M2 screws supplied Repeat this procedure for all color modules Close the remaining openings of the filter wheel with the color mo...
Page 30: ...n the module has been in stalled in the device at Add further dye names if these are not included in the list Click on Accept Follow the above instructions for every installed color module Close the w...
Page 31: ...lect the position of the color module on the carrier in the flourescence measuring head In the Code of module entry field enter the code of the color module In the Dyes input field enter the dye detec...
Page 32: ...nsport Impact and vibration Risk of damage due to shock impact or vibration Large temperature fluctuations Risk of condensation 9 1 1 Inserting the transport lock Before packing the device the transpo...
Page 33: ...ng sign to the packaging CAUTION SENSITIVE ELECTRONIC DEVICE Include a sheet with the following information Name and address of the sender Name and telephone number of a contact for inquiries A detail...
Page 34: ...must be low in dust and free from aggressive vapors If the device is not installed immediately after delivery or not required for prolonged pe riods it should be stored in its original packaging A sui...
Page 35: ...he waste materials that occur during mea surements sample materials in accordance with the statutory and local regulations At the end of its service life the device and all its electronic components m...
Page 36: ...gold plated Block capacity 96 well micro titer plate Sample volume 5 to 100 l Heating Max 8 C s Refrigeration Max 6 C s Temperature setting range 3 to 99 C Heating rate adjustment Min 0 1 C s Tempera...
Page 37: ...T module 1 844 00526 0 FAM donor TAMRA ac ceptor FRET module 2 844 00527 0 FAM donor Cy5 accep tor FRET module 3 844 00528 0 FAM donor Cy5 5 ac ceptor FRET module 4 844 00529 0 JOE donor Cy5 acceptor...
Page 38: ...ing such interference as is liable to cause malfunctions The device meets the re quirements of Part 18 of the FCC regulations Canada regulations The device meets the requirements of Canadian industry...
Page 39: ...beam path through a filter color module 15 Figure 8 Type plate 17 Figure 9 Voltage selection switch on the bottom of the device 20 Figure 10 Positions for additional individual tubes when measuring w...
