EIJKELKAMP 18.65.01, User Manual

Page: 32 / 115

Operating the bbe AlgaeTorch
bbe AlgaeTorch Manual Page 31
1. Offset distilled water. This is used as 0 for the turbidity measurement
2. Offset ultrafiltrated water. This is used as 0 for the chlorophyll measurement. The ultrafiltrated water
contains no algae, but so called yellow substances. This are other fluorescing substances, that are no algae

Avoid artificial light during the calibration, especially fluorescent lamps!

Submerse the AlgaeTorch in the ultra-
filtrated sample water and use “Calibration -> Offsets”
Calibration solution for
the „ultra filtrated water offset and the yellow substances
In the lab, the calibration of the yellow substances can be easily done with tap water. In cases of very small
chlorophyll-a concentration, it is recommended to use ultrafiltered sample of the water (from the water to be
examined). For this the following equipment is needed:
Type of filter: Nitrocellulose filter, pore size 0.45 µm, diameter 47mm (for example: Sartorius Stedim)
Vessels: Depending on the sample volume flask with 5 L content
Negative pressure of 800mbar. The volume of the sample is determined by the instrument to be calibrated, BBE.
For an ALA be about 100ml, requires approximately 5L filtrate for an AOA / AlgaeGuard / A-Tox sensor /
AlgaeTorch about 1L and for an immersion probe.
C
ALIBRATION OF THE ALGAE FINGERPRINTS
:
The calibration of the fingerprints has to be performed in 2 steps: one low calibration point and one high
calibration point. We recommend using 30-50µg/l for the lower point and 100-150µg/l for the higher point. After
calibrating one point, the value is stored for 1 hour. Within this time the second calibration point has to be
measured. Otherwise, the first calibration point will be deleted when re-entering the calibration menu.

Do an offset calibration before calibrating the algae classes.

For the calibration of the algae fingerprints, please use pure solutions of the algae class with a known
chlorophyll concentration. The concentrations should be in the ranges of 30-50µg/l and 100-150µg/l.

Avoid artificial light during the calibration, especially fluorescent lamps!

Submerse the AlgaeTorch in the solution.

Choose “Calibration -> Fingerprints” in the software, choose the algae class

Choose the corresponding low or high concentration
Calibration of:
<Cyano low conc>
OK <- -> ESC

and enter the concentration.
Enter known
Conc.: 036.0 µg/l
OK + - ESC

OK starts the calibration.

Apply the values after the calibration.

Re-perform the same procedure with the high concentration between 100 and 150 µg/l within a
maximum time of one hour.

Re-perform the whole procedure with green algae.