Eickemeyer 173610, User Manual

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USER MANUAL
RETEVET™ PORTABLE ERG
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Protocol
ECVO with ERG-measured DA single
is a shorted version of
ECVO long protocol for PRA diagnosis
protocol where the
dark-adapted tests use only 1 fl ash instead of 4. Replicates can be made in both protocols, and artefactual results deleted.
This protocol is recommended for NON-anesthetized or NON-sedated animals.
ECVO long protocol for PRA diagnosis single fl ash
Description Eye Flash Luminance Energy
(0.33, 0.33 White) Background Luminance
(0.33, 0.33 White) # Flashes
Dark-adapted 0.01 Operator’s choice 0.01 cd·s/m² @ 0.2 Hz Off 1
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 1
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 1
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 1
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 1
Wait remainder of 4 minutes, observe count-down timer, press “Start Test” to continue
Dark-adapted 0.01 0.01 cd·s/m² @ 0.2 Hz Off 1
Dark-adapted 3.0 3 cd·s/m² @ 1 / 15 Hz Off 1
Dark-adapted 10.0 10 cd·s/m² @ 0.05 Hz Off 1
Light adaptation timer Off 30 cd/m²
Light adapted 3.0 3 cd·s/m² @ 2 Hz 30 cd/m² 20
Light adapted 3.0 fl icker 3 cd·s/m² @ 28.3 Hz 30 cd/m² 141 –424
PupilLightRefl ex protocol generate 1000 cd/m² red (621 nm) or blue (470 nm) light, as selected by the operator so the pupil
responses can be observed on the display and noted down. No report is generated. This protocol should not be used if the
animal has been artifi cially dilated, as the pupils will be unresponsive.
Iscev-Based ERG Protocols for Animal Use
These protocols are created for research and pharmaceutical testing. They use standards published for humans by International
Society for Clinical Electrophysiology of Vision (ISCEV) and are most suitable for basic discovery, pre-clinical, and toxicology
studies.
The following tables describe other ERG protocols for animal use.
In the protocol
Dog, Cat, Nonhuman Primate 6 step, light adapted fi rst
, light adapted tests are performed fi rst. The 10-min
light adaptation in the waiting or examination room ambient light is often considered suffi cient. A minimum dark-adaptation
period of 20 minutes is suggested to measure rod function.
Oscillatory potentials, the 6
th
step in the protocol, are automatically extracted and measured from the dark-adapted 3.0 test.