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Dynamica HALO XB-10/BIOmaster/VIS-20/ENVmaster Instruction Manual Download Page 49

XB-10/BIOmaster/VIS-20/ENVmaster 

instruction manual 

 

 

   

 

 

 

 

 

 

 

 

 

48   

BIOmaster protein analysis interface 

CALIBRATION

（

concentraton

）

standard sample setting interface in BRADFORD 

parameter setting interface 

 

 

STD1~3 

standard sample concentration setting

（

According to the number of concentration in the upper 

layer interface, it will display corresponding setting

）

 

OK 

Finish parameter setting, back to measure interface automatically   

Save 

save parameter (SD card) 

 

Summary of Contents for HALO XB-10/BIOmaster/VIS-20/ENVmaster

Page 1: ...HALO XB 10 VIS 20 BIOmaster Instruction manual Accessories for Model HALO XB 10 VIS 20 BIOmaster UV VIS Spectrophotometer Revision D01 201407 ...

Page 2: ...hase of Dynamica XB 10 BIOmaster VIS 20 ENVmaster spectrophotometer In order to ensure proper use and your safety please read this manual carefully and keep it well before using the instrument Information contained in this manual is subject to change without notice for product appearance and technical data Enquiries are welcome ...

Page 3: ... that to which the radio TV receiver is connected 2 Possible Electromagnetic Wave Interference Affecting This Instrument If this instrument is used near an intense electromagnetic source interference noise may be given to the instrument to incur an adverse effect on its performance or functionality To prevent this use the specified system connection cables in strict accordance with the instruction...

Page 4: ...at the time of replacement 2 Warranty Period One year from the date of initial installation 3 Availability of Technical Support Service Technical support service for this instrument is available within regular working hours on workdays predetermined by us 4 Limitations and Exclusions on Warranty Note that this warranty is void in the following cases even if they occur within the warranty period a ...

Page 5: ...or damage to the system software application software data or hard disk due to disconnection of main power to the personal computer without taking the specified normal shutdown procedure 5 Disclaimer of Warranty a Any express warranties other than the explicit conditions indicated in 1 are excluded from this warranty b Any other implied warranties of merchantability and fitness for a particular pu...

Page 6: ...icals and samples used in analytical operations of this instrument b Reagents standard solutions and accuracy control samples shall be handled stored and discarded as instructed by the respective suppliers 2 Notice on Instruction Manuals a Information contained in the instruction manuals furnished with the instrument is subjected to change without notice for product improvement b This manual is co...

Page 7: ...pear on the warning labels on the product or in the manual have one of the following alert headings consisting of an alert symbol and a signal word DANGER WARNING or CAUTION DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury It does not apply to this equipment WARNING Indicates a potentially hazardous situation that if not avoided can resu...

Page 8: ...d to present warnings which are not directly related to personal injury hazards It is used to indicate prevention against damage to the equipment Note This is used to indicate instructions that enable you to operate the equipment accurately and perform accurate measurements ...

Page 9: ...afety in this manual If you do not follow the instructions in this manual an inaccurate analysis may result or bodily injury may occur Because of danger please make sure not to modify or alter the product make sure not to use unspecified parts and make sure not to operate the equipment by removing defeating the safety device s When using chemicals please make sure to ventilate the room well If the...

Page 10: ... the instruction of the installer Installation of this instrument shall be carried out by or under supervisions of qualified service personnel of Dynamica or its authorized service agent When relocation of this instrument becomes necessary after initial installation delivery please notify your local Dynamica sales representative or nearest Dynamica service office Technical support service for this...

Page 11: ... solvent vapor may be harmful to your health WARNING Eye Injury from Organic Solvents Handling Organic Solvents Please wear protective glasses when using organic solvents If the organic solvent should get into your eye flush your eye immediately under running water for at least 5 minutes while keeping your eyelids open See a physician for appropriate treatment ...

Page 12: ...tch off the power cord is pulled off and the D2 lamp and the tungsten lamp is cooled off when replacing the lamp CAUTION Fatigue due to Prolonged Work Viewing the display in your work can cause eye and physical fatigue if you continue to work in the same posture for extended periods When working with the display for a prolonged period for your health make sure to take breaks for 10 to 15 minutes e...

Page 13: ...istance of less than 4 Defective grounding may not only cause lower resistance against noise from the outside but it can also cause the Mass Spectrometer and personal computer to generate static electricity which may involve the danger of electrical shocks c A high voltage circuit is used inside the Spectrophotometer Do not open the covers when this circuit is operational because of the danger fro...

Page 14: ...rval 为 is 0 2nm its scanning speed is 2400nm min Unique application method manager changes the original function oriented operation method and user sample measurement method operation mode Energy saving and environmental protection High conversion efficiency switching power supply 100 240VAC voltage input and complete riddance of low grid voltage VIS 20 EVNmaster spectrophotometer uses 2000 hours ...

Page 15: ...14 Appearance Keypad Though the instrument uses touch display screen all operation will be acheived by touch screen The purpose of setting the two shortcut key is to make easy operation BACK Return to the previous menu MEASURE Begin to a new measurement ...

Page 16: ...hat the instrument is used under 30 70 humidity Storage humidity less than 85 Workbench Width more than 550mm Depth more than 400mm Load bearing more than 15kg horizontal and reliable workbench If there are other auxiliary devices it is necessary to enlarge workbench area More than 200mm space at the both sides of the instrument avoid being close to the wall Height of the instrument about 160mm In...

Page 17: ... stability 0 0008Abs h 500nm 2 hours after power on Spectral bandwidth 6nm 1 2nm Stray light 0 3 T Wavelength scan speed 2400nm min 0 2nm sampling interval without filter switchover Wavelength move speed To any specified position within 1 second Light source WI lamp Detector silicon photodiode Display screen 4 3 inches 480 272 colorful touch LCD screen Printer specified 80 column thermal printer s...

Page 18: ...ility 0 005Abs h 250nm 2 hours after light on Spectral bandwidth 5nm 1nm Stray light 0 5 T Wavelength scan speed 300nm min 0 2nm sampling interval without filter switchover Wavelength move speed To any specified position within 1 second Light source pulsed xenon lamp Detector silicon photodiode Display screen 4 3 inches 480 272 colorful touch LCD screen Printer specified 80 column thermal printer ...

Page 19: ...ays Spectrogram button These buttons appear on scan interface For the results of spectrogram processing Status bar These buttons appear on every interface Show current time wavelength and vaule transmission or absorbance Click wavelength in the status bar it will pop a digital input interface then set up wavelength to achevie GOTO λ function Click value in the status bar it will pop a big font mea...

Page 20: ...can input word for example sample name user and document name BACK close word input keypad CLEAR clear incorrect word Word switchover majuscule lowercase digit and sign Enter ensure input word Digit input keypad This keypad appears at the area where can input digit for example wavelength value and scanning time ...

Page 21: ...nterface Click icon into the corresponding measure interface ABS T wavelength photometric WL SCAN wavelength scanning TIME SCAN time scanning CONC concentration regression MANAGER measure method manger including data saving except VIS 20 SETTING parameter setting ...

Page 22: ...lick value in the status bar into the large character measure T button absorbance or transmission mode ZERO button zero absorbance blank sample PRINT button print data READ button only for XB 10 BIOmaster spectrophotometer to start a new measure In any interface non execution of measure in the interface click wavelength in the status bar input wavelength the instrument will move to the target wave...

Page 23: ... measure specified wavelength s absorbance or transmission The wavelength value is less than 6 Each measure document includes 200 maximum measure results Measure buttons READ begin to measure SET parameter setting ZERO zero absorbance blank sample LOAD load original measure results or measure method document PRN print data ...

Page 24: ... including T or ABS PATHLENGTH cuvette optical length it can print but cannot regulate absorbance value SAMPLE NAME sample name less than 8 characters WL NUMBER wavelength quantity less than 6 WL1 WL6 wavelength wavelength value allow not sort OK finish parameter setting back to measure interface automatically Save save parameter ...

Page 25: ...ngth the purpose of energy scan is to judge the instrument current status Functions the peak value judge smooth and constant computing peak area calculation spectrogram data point cursor tracking spectrogram automatic and manual zoom of the scan results Measure buttons READ begin to measure SET parameter setting ZERO zero baseline blank sample LOAD load original measure results or measure method d...

Page 26: ... end position INTERVAL nm scan interval AUTO 0 2nm 0 5nm 1nm 2nm 5nm AUTO interval when scan range is less than 500nm and time interval is 0 5nm When scan range is more than 500nm and time interval is 1nm Peak value judge large wavelength interval adapts to judging flat peak small one adapts to judge keen edged peak Y MAX ordinate ceiling of spectrogram Y MIN ordinate low of spectrogram the instru...

Page 27: ...on Smooth the spectrogram less than 6 smoothness Calcu Calculation function Addition subtraction multiplication and division Area Peak area calculation function According to the setting wavelength range calculate peak area Digit tracking button Show wavelength and value Automatic scaling button According to measured value adjust longitudinal coordinates to the proper range Spectrogram enlargement ...

Page 28: ...ission absorbance and the time Functions dynamics calculation smooth and constant computing peak area calculation spectrogram data point cursor tracking spectrogram automatic and manual zoom of the scan results Measure buttons READ begin to measure SET parameter setting ZERO zero baseline blank sample LOAD load original measure results or measure method document PRN print data ...

Page 29: ... is less than 1000s scan interval is 1s When scan time is 1000 2000s scan interval is 2s When scan time is 5000 20000s scan interval is 10s When scan time is more than 20000s scan interval is 100s Y MAX ordinate ceiling of spectrogram Y MIN ordinate low of spectrogram the instrument will automatically adjust the small value to the ordinate low position PATHLENGTH cuvette optical length it can prin...

Page 30: ...ke of spectrogram smooth processing Calcu Calculation function Addition subtraction multiplication and division Area Peak area calculation function According to the setting wavelength range calculate peak area Digit tracking button Show wavelength and value Automatic scaling button According to measured value adjust longitudinal coordinates to the proper range Spectrogram enlargement button Zoom 1...

Page 31: ...uantity is less than 9 The times of standard sample average is less than 5 Regression spectrogram and mathematical equation is displayed The concentration regression parameter can be input directly Regression standard sample and measure document can be saved in the SD card Each measure document includes 200 maximum measure results Measure buttons READ begin to measure SET parameter setting ZERO ze...

Page 32: ...on calculation method One is concentration regression according to standard sample the other is concentration calculation according to regression equation parameters According to different concentration calculation methods parameter setting will make automatic adjustments STD NUMBER Standard sample number Less than 9 REPLICATES Standard sample average measure times Less than 5 OK Finish parameter ...

Page 33: ...ace STD1 STD9 9 standard sample concentration concentration must be sorted in general sort ascending If using regression equation parameters to enter calculation method CALIBRATION select K Factory it will show this parameter setting interface K1 Slope value of regression equation K0 Intercept value of regression equation OK Finish parameter setting back to measure interface automatically BACK Bac...

Page 34: ...nd open previous measure methods Use this function user must load SD card Measure methods and measure results document are saved in SD card METHOD measure methods manger ABS T Given wavelength direct reading method WL SCAN Wavelength scan method TIME SCAN Time scan method CONC Given wavelength concentration regression method Open Open selected measure method New Renew selected measure method ...

Page 35: ...thod documents are saved in SD card Method document display saved method document Preview display selected method document Open open selected method document Delete delete selected method document Next display method document at next page Back display method document at prior page Method document type PRP Given wavelength direct reading method document PRW Wavelength scan method document PRT Time ...

Page 36: ...e user must load SD card Measure data is saved in SD card DATA measure data document manager interface Open open selected data document Delete delete selected data document Next display data at next page Back display data at prior page Data document type PGT Given wavelength direct reading data document WSC Wavelength scan data document TSC Time scan data document CON Given wavelength concentratio...

Page 37: ...FF display main interface do not enter into any measure interface RTD enter into big font T A direct reading interface ABS T enter into given wavelength T A direct reading interface WL SCAN enter into wavelength scan interface TIME SCAN enter into time scan interface INITIALZE Initialize button Click this button to initialize Before clicking the sample need be taken out of the chamber and remain e...

Page 38: ...wer down protection capacity in general about 96 hours Switching off the instrument for 96 hours the user need to reset time but the setting data will be not lost YEAR the last two digits MONTH month DAY date HOUR hour 24 hour format MINUTE minute FORMAT date format YY MM DD year month day MM DD YY month day year DD MM YY day month year ...

Page 39: ... used to check instrument s performance Finishing test it will display measure result automatically and judge results It meets to routine application standards NOISE noise level test STABILITY stability test preheating for 2 hours BASELINE FLATNESS baseline flatness test HARDWARE hardware self check Print print test data ...

Page 40: ...rface it also own measure funcitions of traditonal spectrophotometer BIOmaster mian interface Click the icon enter into corresponding measure interface DNA RNA nucleic acids analysis PROTEIN protein determination OD600 Bacterial cell culture measurement SPECTRUM Traditional spectrophotometer function MANAGER measure method manager including data saved SETTING parameter setting ...

Page 41: ...hods in the DNA RNA nucleic acids analysis interface dsDNA ssDNA RNA OLIGO Click button to select measure method Measure buttons READ being to measure SET parameter setting ZERO zero absorbance blank sample at all setting wavelength point LOAD load original measure results or measure method document PRN print data ...

Page 42: ...ace FACTOR regulate parameters BACKGROUND background calibration PATHLENGTH optical length of cuvette optical length is less than 10mm DI FACTOR dilution factor UNIT concentration units SAMPLE NAME sample name OK Finish parameter setting back to measure interface automatically Save save parameter SD card ...

Page 43: ...otein determination interface PROTEINUV BRADFORD BSA LOWRY BIURET Click button to select measure methods PROTEINUV functions Measure buttons READ begin to measure SET parameter setting ZERO zero absorbance blank sample at all setting wavelength point LOAD load original measure results or measure method document PRN print data ...

Page 44: ...0nm position A260 FACTOR correction factor at 260nm position PATHLENGTH optical length of cuvette optical length is less than 10mm BACKGROUND background calibration PROTEIN select measure methods DI FACTOR dilution factor UNIT concentration units SAMPLE NAME sample name OK Finish parameter setting back to measure interface automatically Save save parameter SD card ...

Page 45: ...V parameter setting interface WL nm Wavelength value MOLAR EXT C Molar extinction conefficient correction factor MOLECULAR W Molecular weight molecular weight According to the two correction factors calculate correction factor at A280 FACTOR automatically OK Finish parameter setting back to measure interface automatically Back Back to the prior parameter setting interface ...

Page 46: ...n measure method in PROTEINUV parameter setting interface WL nm Wavelength value EXT COEFF Extinction conefficient correction factor According to the correction factors calculate correction factor at A280 FACTOR automatically OK Finish parameter setting back to measure interface automatically Back Back to the prior parameter setting interface ...

Page 47: ...ster protein analysis interface BRADFORD measure interface Measure buttons READ begin to measure SET parameter setting ZERO zero absorbance blank sample at all setting wavelength point LOAD load original measure results or measure method document PRN print data ...

Page 48: ...PATHLENGTH optical length of cuvette optical length is less than 10mm CALIBRATION concentration regression way STD NUMBER concentration standard sample number REPLICATES measure times DI FACTOR dilution factor UNIT concentration units SAMPLE NAME sample name OK Finish parameter setting back to measure interface automatically Save save parameter SD card ...

Page 49: ...andard sample setting interface in BRADFORD parameter setting interface STD1 3 standard sample concentration setting According to the number of concentration in the upper layer interface it will display corresponding setting OK Finish parameter setting back to measure interface automatically Save save parameter SD card ...

Page 50: ...cterial cell culture measurement interface OD600 measure interface Measure buttons READ begin to measure SET parameter setting ZERO zero absorbance blank sample at all setting wavelength point LOAD load original measure results or measure method document PRN print data ...

Page 51: ...asurement interface OD600 parameter setting interface I WL nm Test wavelength PATHLENGTH optical length of cuvette optical length is less than 10mm UNIT concentration units SAMPLE NAME sample name OK Finish parameter setting back to measure interface automatically Save save parameter SD card ...

Page 52: ...select UNIT cells ml it will display above parameter setting interface WL nm Test wavelength PATHLENGTH Optical length of cuvette optical length is less than 10mm UNIT Concentration units SAMPLE NAME Sample name FACTOR Correction factor MULTIPLIER Multiple OK Finish parameter setting back to measure interface automatically Save Save parameter SD card ...

Page 53: ...XB 10 BIOmaster VIS 20 ENVmaster instruction manual 52 BIOmaster spectrum interface ABS T given wavelength photometry direct reading measure interface Wavelegnth scan interface ...

Page 54: ...XB 10 BIOmaster VIS 20 ENVmaster instruction manual 53 BIOmaster spectrum interface KINETICS dynamics measure interface CONC concentration regression and mesure interface ...

Page 55: ... functions Auto sample sipper auto 5 cell holder electronic thermostat TC holder only for XB 10 BIOmaster Remark When installing accessories the power of the instrument must keep off Install and recognize auto 5 cuvette holder interface Click display 5 cell holder setting interface CELL NUM SETUP cuvette number Less than 5 1 5 GOTO CELL specified cuvette position ...

Page 56: ...face Click display auto sample sipper setting interface SIP TIME s sample sipper time UNIT second DELAY TIME s delay time between sample sipper and measure PURGE TIME s suck air after finishing measure This feature is primarily used to separate the two samples in the pipeline In order to recycle sample ...

Page 57: ...ion manual 56 Install and recognize electronic thermostat TC holder interface only for XB 10 BIOmaster Click display temperature setting interface TEMPERATURE setting control temperature TEMP CONTROL turn on or off temperature control function ...

Page 58: ...ica eu com Web www dynamica eu com Australasia Dynamica Pty Ltd ABN 16 126 043 052 PO Box 3278 Prahran East Victoria 3181 Australia P 61 3 8540 5988 F 61 3 8540 5981 Email info dynamica com au Web www dynamica com au Asia Dynamica Asia Limited Room 607 Yen Sheng Centre 64 Hoi Yuen Road Kwun Tong Kowloon Hong Kong P 852 3583 1581 F 852 3583 1580 Email info dynamica asia com Web www dynamica asia co...

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