Du Pont SORVALL RT6000B, Operating Instructions Manual

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SORVALL
®
Centrifuges
Operation
3-3
Air flow through the T6000B centrifuge is designed to minimize increase in rotor
temperature. Air enters through an opening in the center of the lid and exits through the
exhaust duct at the top of the rotor chamber.
WARNING
Blocking the air flow entering and/or exiting the T6000B rotor chamber
will cause an increase in the temperature within the rotor chamber. The
temperature increase can temporarily distort the non-metal surfaces
allowing particles to exit the rotor chamber resulting in personal injury and/
or centrifuge damage should tube breakage or rotor failure occur.
When running temperature-sensitive material we recommend that you do a trial run.
b. Rotor Installation, Loading and Balancing
Refer to the rotor instruction manual for loading and balancing procedures as well as
information regarding the selection and use of tubes, bottles, and adapters.
WARNING
When loading the rotor, be sure not to exceed the maximum compartment
mass of the rotor (see Table 1-1 or the individual rotor instruction manual).
If maximum compartment mass is exceeded, maximum rotor speed must be
lowered as described on page 3-6, Reducing Speed for Rotor Compartment
Loads in Excess of Design Mass. Failure to do so can cause rotor failure
which could result in personal injury and/or centrifuge damage.
CAUTION
Do not operate the centrifuge without a rotor installed and properly
balanced. Also, when installing a rotor carefully place it on the centrifuge
drive spindle. The drive can be damaged if rotors are dropped onto it.
Precooling a Rotor in the RT6000B Centrifuge
For operation at temperatures other than ambient, the rotor and rotor chamber should
be precooled before the run using either of the precooling methods described.
a. Precooling a Rotor in the Centrifuge
1. Follow steps 1 through 6 of the operating instructions (pages 3-4 and 3-5).
2. Set the
REV/MIN x 1000
dial to 1500 rpm.
The length of time it will take to precool a rotor will vary depending on the temperature
selected, rotor weight, and rotor material.