DMT 750 TOBS Page 6 User Manual Download

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Figure A: 20ml Tissue chamber

1.3 TISSUE ORGAN BATH SYSTEM - 750 TOBS: CHAMBER

NOTE: The Chamber Detector registers when the tissue chamber is placed. If the chamber
is placed correctly the blue indicator lamp will be lit and the tissue organ bath will work.

Tissue chamber

Chamber detector

Air heating

Sample holder

(lower part shown)

Summary of Contents for 750 TOBS

Page 1: ...USER GUIDE VOL 2 0 TISSUE ORGAN BATH SYSTEM 750TOBS ...

Page 2: ......

Page 3: ...ystem 750 TOBS Chamber 6 CHAPTER 2 SETTING UP THE TISSUE ORGAN BATH 8 2 1 Tissue chamber handling procedure 8 2 2 Adjusting the transducer 9 2 3 Different types of tissue sample holders 10 2 4 Isometric Transducer 11 CHAPTER 3 CLEANING AND MAINTENANCE 12 3 1 Cleaning the Tissue Organ Bath System 12 APPENDIX 1 BUFFER RECIPES 14 Physiological Saline Solution PSS 14 ...

Page 4: ...BATH SYSTEM 750 TOBS FRONT PANEL Perfusion buffer inlet Mounted to the lid inside the bottles A constant flow shunt or a fill empty shunt Tissue chamber Sample holder Chamber door Glass presence indicator Oxygenation CO2 regulator Power indicator Front door Transducer ...

Page 5: ...TOBS REAR PANEL Communication indicator USB connection Analogue recorder output Suction connection for vacuum pump Oxygen Carbogen Pressure inlet max 10bar 145psi 115 230V 50 60Hz automatic voltage selector ON OFF switch Power connector ...

Page 6: ... TOBS CHAMBER NOTE The Chamber Detector registers when the tissue chamber is placed If the chamber is placed correctly the blue indicator lamp will be lit and the tissue organ bath will work Tissue chamber Chamber detector Air heating Sample holder lower part shown ...

Page 7: ...s are turned at regular intervals to prevent them from getting stuck Use ONLY the grease that came with the system Perfusion Buffer inlet Temperature Probe Glass indicator On Glass mounted Off Glass removed Knob for dismounting chamber disc and temperature probe Oxygenation CO2 regulator Oxygenation Carbogen airpipe tubing Drainage tube ...

Page 8: ... chamber disc whilst aligning the pipe stub to the stub opening Gently place the pipe stub situated on the inside of the heating compartment and secure in 3 Removing the glass Carefully pull the top of the chamber outwards pressing down on the bottom of the chamber a few millimeters until the chamber is free to move upwards Then lift out the chamber 1 Move the transducer and mounting supports hold...

Page 9: ... by rotating the locking bolt Locking bolt 1 2 3 4 3 Horizontal adjustment of the transducer Turn the screw clockwise to free the transducer The transducer can now be moved horizontal Turn the screw counterclockwise to lock the transducer 4 Course adjustment of the lower tissue holder Turn the screw counter clockwise to unlock the tissue holder When unlocked it is possible to move the holder in 4 ...

Page 10: ...ulation electrodes optional Figure B Closing clips optional Figure C Pins optional Figure A Triangle hooks standard NOTE The above mounting supports are only examples of supports delivered by DMT If other mounting supports are needed ask your DMT sales representative 2 3 DIFFERENT TYPES OF TISSUE SAMPLE HOLDERS ...

Page 11: ...ucer calibration is performed before the first use of the 750 TOBS system As part of the general maintenance of the 750 TOBS system DMT also recommends that the bath is weight calibrated at least once every month every time the system has been moved or has not been used over a long period The weight calibration procedure is described in detail in chapter 4 in the User Manual 2 4 ISOMETRIC TRANSDUC...

Page 12: ...owing procedure to clean the Tissue Organ Bath chamber tubing and tissue holders 1 Turn pressure OFF 2 Use clean Schott Duran bottles 3 Fill with distilled water 4 Turn Pressure ON 5 Rinse the silicone tubing with double distilled water by activating the Fill Empty Control several times 6 Change the tubing after use of unknown or toxic chemicals or if any kind of hydrophobic reagent has been used ...

Page 13: ...isconnect the temperature probe see figure 3 1 below and take out the chamber disc Clean both with double distilled water and dry with a paper towel IMPORTANT In exceptional cases it may be necessary to demount the sample holders for cleaning to make sure that all surfaces are cleaned Temperature probe Figure 3 1 Chamber disc and temperature probe ...

Page 14: ...double distilled H2 O as three individual solutions as described in the table above Gently heat solution 3 to dissolve the EDTA 2 Solution 1 is added to a graduated bottle and the bottle is filled with double distilled H2 O to a final volume of 500 ml 3 Solution 3 is added to the graduated bottle which afterwards is filled with additional double distilled H2 O to a final volume of 850 ml 4 Aerate ...

Page 15: ...distilled H2 O in a 1000 ml graduated bottle Dissolve the chemicals for solutions 2 and 3 in 75 ml double distilled H2 O in individually cylinders Gently heat solution 3 to dissolve the EDTA 2 Solution 2 and 3 is added to solution 1 and the graduated bottle is filled with additional double distilled H2 O to reach a final volume of 1000 0 ml Before use 3 Dilute the 25 x PSS stock solution 1 25 usin...

Page 16: ... three individual solutions as described in the table above Gently heat solution 3 to dissolve the EDTA 2 Solution 1 is added to a graduated bottle and the bottle is filled with double distilled H2 O to a final volume of 500 ml 3 Solution 3 is added to the graduated bottle that is filled with additional double distilled H2 O to a final volume of about 850 ml 4 Aerate the solution with carbogen 95 ...

Page 17: ...H2 O in a 1000 ml graduated Dissolve the chemicals for solutions 2 and 2 3 in 75 ml double distilled H2 O in individually cylinders Gently heat solution 3 to dissolve the EDTA 3 Solutions 2 and 3 are added to solution 1 and the graduated bottle is filled with additional double distilled H2 O to reach a final volume of 1000 ml Before use 4 Dilute the 25 x PSS stock solution 1 25 using double distil...

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